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andor_revolution [2016/04/17 01:36]
bioimaging created
andor_revolution [2021/01/17 04:48]
bioimaging [Microscope Turn Off Procedure]
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​http://​www.imm.fm.ul.pt/​booking/​month.php?​user=ANDORREVO&​rid=46"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Andor Revolution Booking</​a>​+&rarr; <a href="​http://​www.imm.fm.ul.pt/​booking/old/​month.php?​user=ANDORREVO&​rid=46"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ Andor Revolution Booking</​a>​
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=andor_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Andor Revolution Usage Statistics</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=andor_usage"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=chart_line.png"> ​ Andor Revolution Usage Statistics</​a>​
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 The Andor Revolution //spinning disk confocal microscope//​ is a fast imaging system which provides a trade-off between confocality,​ resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a [[http://​zeiss-campus.magnet.fsu.edu/​tutorials/​spinningdisk/​yokogawa/​yokogawatutorialfigure1.jpg|spinning disk with many small pinholes]]. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control and a small stage incubator for CO2 supply. A UV laser is attached to the back port of the microscope for spot laser ablation. With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Even though its resolution is not as high as a //​point-scanning confocal system// such as the [[zeiss_lsm_510_meta|Zeiss LSM 510 META]] or [[zeiss_lsm_710|Zeiss LSM 710]], it is much faster than these two systems. If you need to use an upright microscope, check the [[zeiss_lsm_7_live|Zeiss LSM 5 Live]] fast confocal system instead. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]]. ​ The Andor Revolution //spinning disk confocal microscope//​ is a fast imaging system which provides a trade-off between confocality,​ resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a [[http://​zeiss-campus.magnet.fsu.edu/​tutorials/​spinningdisk/​yokogawa/​yokogawatutorialfigure1.jpg|spinning disk with many small pinholes]]. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control and a small stage incubator for CO2 supply. A UV laser is attached to the back port of the microscope for spot laser ablation. With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]. Even though its resolution is not as high as a //​point-scanning confocal system// such as the [[zeiss_lsm_510_meta|Zeiss LSM 510 META]] or [[zeiss_lsm_710|Zeiss LSM 710]], it is much faster than these two systems. If you need to use an upright microscope, check the [[zeiss_lsm_7_live|Zeiss LSM 5 Live]] fast confocal system instead. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]]. ​
  
-  * **Microscope**:​ [[http://www.nikon.com/products/instruments/lineup/biological-microscopes/​inverted/​ti_eus/​index.htm|Nikon Eclipse Ti-E]]+  * **Microscope**:​ [[https://www.nikoninstruments.com/en_EU/Products/Inverted-Microscopes/Eclipse-Ti-E|Nikon Eclipse Ti-E]]
   * **Confocal scanner**: [[http://​www.andor.com/​microscopy_systems/​peripherals/​confocal_scanners/​|Yokogawa CSU-x1]]   * **Confocal scanner**: [[http://​www.andor.com/​microscopy_systems/​peripherals/​confocal_scanners/​|Yokogawa CSU-x1]]
-  * **Camera**: [[http://​www.andor.com/​scientific_cameras/ixon/models/​default.aspx?​iProductCodeID=3|Andor iXon 897 EMCCD]]+  * **Camera**: [[http://​www.andor.com/​scientific-cameras/ixon-emccd-camera-series/ixon-ultra-897|Andor iXon 897 EMCCD]]
  
  
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   * Switch off the computer   * Switch off the computer
   * Turn off all system components (no particular order)   * Turn off all system components (no particular order)
- 
-===== TOP 5 Users ===== 
- 
-^  2012  ^^  2011  ^^ 2010 ^^ 2009 ^^ 
-|  Elsa Abranches (UBD)  |  170.5h ​ |  Lara Carvalho (UMO)  |  376h  |  Lara Carvalho (UMO)  |  399.5h ​ |  Dulce Azevedo (UMO)  |  255h  | 
-|  Lara Carvalho (UMO)  |  133h  |  Soren Prag (UMO)  |  372h  |  Dulce Azevedo (UMO)  |  387.5h ​ |  João Cordeiro (UMO)  |  199.5h ​ | 
-|  Marco Antunes (UMO)  |  98.5h  |  Marco Antunes (UMO)  |  170.5h ​ |  João Cordeiro (UMO)  |  358h  |  Ghislain Cabal (UMA)  |  111h  | 
-|  Carolina Moreira (UMO)  |  76h  |  Célia Carvalho (UBCe) ​ |  152h  |  Soren Prag (UMO)  |  272.5h ​ |  Isabel Campos (UMO)  |  101.5h ​ | 
-|  Soren Prag (UMO)  |  60.5h  |  João Cordeiro (UMO)  |  147h  |  Marco Antunes (UMO)  |  256h  |  Marco Antunes (UMO)  |  100h  | 
  
 ---- ----
-[[groups:​bioimage:​resources|Back to the Resources ​page]]+[[resources|Back to the Equipment ​page]]
andor_revolution.txt · Last modified: 2021/01/17 04:48 by bioimaging