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about [2022/06/19 16:39] bioimaging |
about [2024/01/28 13:58] (current) bioimaging |
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→ **Meet the** [[team|{{team_neg.png?0x35|}}]] | → **Meet the** [[team|{{team_neg.png?0x35|}}]] | ||
- | We are a node of the [[https://www.ppbi.pt/joomla30/index.php/ppbi|Portuguese Platform of Bioimaging]], which integrates the European research infrastructure as a Biological Node of [[https://www.eurobioimaging.eu/|Euro-BioImaging ERIC]]. We are also a [[https://www.zeiss.com/microscopy/int/about-us/microscopy-customer-center/labs-location-partner.html|Zeiss labs@location Partner]] and one of the [[https://colife.eu/en/research/core-facilities/bioimaging/|COLife]] Bioimaging Core Facilities. | + | We are a node of the [[https://www.ppbi.pt/joomla30/index.php/ppbi|Portuguese Platform of Bioimaging (PPBI)]], a nationwide consortium of imaging facilities which integrates the European research infrastructure as a Biological Node of [[https://www.eurobioimaging.eu/|Euro-BioImaging ERIC]]. We are also a [[https://www.zeiss.com/microscopy/int/about-us/microscopy-customer-center/labs-location-partner.html|Zeiss labs@location Partner]], integrating a community of advanced microscopy facilities providing in-depth knowledge, dedicated services across a wide range of applications and instrumentation, and scientific support to ZEISS customers. |
===== How to Acknowledge us ===== | ===== How to Acknowledge us ===== | ||
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===== Services ===== | ===== Services ===== | ||
- | The Bioimaging Unit is equipped with 2 inverted laser point-scanning confocal microscopes prepared for live cell imaging ([[zeiss_lsm_880|Zeiss LSM 880]] and [[zeiss_lsm_710|Zeiss LSM 710]]), 1 lightsheet microscope prepared for water and clearing media immersion imaging ([[zeiss_lightsheet_z1|Zeiss Lightsheet Z1]]), 2 inverted spinning disk confocal microscopes with motorized stages, piezo and EM-CCDs also prepared for live cell imaging ([[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]), 1 multi-photon microscope ([[leica_sp8_mp|Leica SP8 MP]]), 3 fully motorized inverted widefield microscopes with cooled CCD cameras ([[zeiss_cell_observer|Zeiss Cell Observer]] with a large cage incubator, [[nikon_eclipse_ti|Nikon Eclipse Ti]] with stage top incubation, and [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]), 1 upright widefield fluorescence microscope with a CCD camera ([[leica_dm5000b|Leica DM5000B]]), 1 fluorescence stereo microscope with a CCD camera ([[zeiss_axiozoom_v16|Zeiss AxioZoom V16]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescent/fluorescence imaging system ([[ivis_lumina|IVIS Lumina]]). There is also a high-spec workstation for image analysis and processing available for researchers to work on their imaging data ([[colossus|Colossus]]). The Bioimaging systems are available on the [[https://imm.agendo.science|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience. | + | {{graphical_abstract_2022_dark.png?0x400 |}} The Bioimaging Unit is equipped with 2 inverted laser point-scanning confocal microscopes prepared for live cell imaging ([[zeiss_lsm_980|Zeiss LSM 980]], and [[zeiss_lsm_880|Zeiss LSM 880]], 1 lightsheet microscope prepared for water and clearing media immersion imaging ([[zeiss_lightsheet_z1|Zeiss Lightsheet Z.1]]), 2 inverted spinning disk confocal microscopes with motorized stages, piezo and EM-CCDs also prepared for live cell imaging ([[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]), 1 multi-photon microscope ([[leica_sp8_mp|Leica SP8 MP]]), 1 automated widefield microscope for live cell imaging ([[zeiss_celldiscoverer_7|Zeiss Celldiscoverer 7]]), 3 fully motorized inverted widefield microscopes with cooled CCD cameras ([[zeiss_cell_observer|Zeiss Cell Observer]] with a large cage incubator set up with an AKOYA Phenocycler fluidics system, [[nikon_eclipse_ti|Nikon Eclipse Ti]] with stage top incubation and a Ionoptix high-speed calcium and contractility system, and [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]), 1 fluorescence stereo microscope with a CCD camera ([[zeiss_axiozoom_v16|Zeiss Axio Zoom.V16]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescence / fluorescence imaging system ([[ivis_lumina|IVIS Lumina]]). There is also a high-spec workstation for image analysis and processing available for researchers to work on their imaging data ([[chronos|Chronos]]). The Bioimaging systems are available on the [[https://imm.agendo.science|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience. |
→ **More info on our** [[resources|{{equipment_neg.png?0x35|}}]] | → **More info on our** [[resources|{{equipment_neg.png?0x35|}}]] | ||
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=== Hours === | === Hours === | ||
- | **9:00 - 13:00 and 14:00 - 18:00** for **Training, Assistance and Consultations** | + | **9:00 - 13:00 and 14:00 - 17:30** for **Training, Assistance and Consultations** |
24 hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Policies]]) | 24 hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Policies]]) |