User Tools

Site Tools


about

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
Next revision
Previous revision
about [2022/02/12 23:56]
bioimaging
about [2024/01/28 13:58] (current)
bioimaging
Line 1: Line 1:
 ====== About the Bioimaging Unit ====== ====== About the Bioimaging Unit ======
  
-{{::​team_photo_2020.jpg?​550 ​ |}} \\ \\ \\ \\ \\ The Bioimaging Unit acts as a support structure to help and nurture research done with Light MicroscopyThe people we work with have amazing questions to answer. But if used incorrectly,​ a microscope can give you wrong answers. At the Bioimaging Unit we focus on people and their questions. We provide iMM scientists and visitors with excellence ​in scientific know-how and expertise in light microscopy methods, in a personalized approach. We sit with researchers to teach them, train them, create software tools for them and help them make the most out of the microscope. We provide support in all steps of research done with Light Microscopy. We help researchers get to that moment when something is seen for the first time. To see Science done right, see it happening. That’s unique.+<​html>​ 
 +<iframe width="​560"​ height="​315"​ src="​https://www.youtube.com/​embed/​9ntTKoIexF4"​ title="​YouTube video player"​ frameborder="​0"​ allow="​accelerometer;​ autoplay; clipboard-write;​ encrypted-media;​ gyroscope; picture-in-picture"​ allowfullscreen></​iframe>​ 
 +</​html>​
 \\ \\
-Photo: [[https://​www.jorgefiganiercastro.com/|Jorge Figanier Castro]]+The Bioimaging Unit acts as a support structure to help and nurture research done with Light MicroscopyThe people we work with have amazing questions to answer. But if used incorrectly,​ a microscope can give you wrong answers. At the Bioimaging Unit we focus on people and their questions. We provide iMM scientists and visitors with excellence in scientific know-how and expertise in light microscopy methods, in a personalized approach. We sit with researchers to teach them, train them, create software tools for them and help them make the most out of the microscope. We provide support in all steps of research done with Light Microscopy. We help researchers get to that moment when something is seen for the first time. To see Science done right, see it happening. That’s unique.
 \\ \\
 \\ \\
 → **Meet the** [[team|{{team_neg.png?​0x35|}}]] → **Meet the** [[team|{{team_neg.png?​0x35|}}]]
  
-We are a node of the [[https://​www.ppbi.pt/​joomla30/​index.php/​ppbi|Portuguese Platform of Bioimaging]],​ which integrates the European research infrastructure as a Biological Node of [[https://​www.eurobioimaging.eu/​|Euro-BioImaging ERIC]]. We are also a [[https://​www.zeiss.com/​microscopy/​int/​about-us/​microscopy-customer-center/​labs-location-partner.html|Zeiss labs@location Partner]] ​and one of the [[https://​colife.eu/​en/​research/​core-facilities/​bioimaging/​|COLife]] Bioimaging Core Facilities.+We are a node of the [[https://​www.ppbi.pt/​joomla30/​index.php/​ppbi|Portuguese Platform of Bioimaging ​(PPBI)]], a nationwide consortium of imaging facilities ​which integrates the European research infrastructure as a Biological Node of [[https://​www.eurobioimaging.eu/​|Euro-BioImaging ERIC]]. We are also a [[https://​www.zeiss.com/​microscopy/​int/​about-us/​microscopy-customer-center/​labs-location-partner.html|Zeiss labs@location Partner]], integrating a community ​of advanced microscopy facilities providing in-depth knowledge, dedicated services across a wide range of applications and instrumentation,​ and scientific support to ZEISS customers.
  
 ===== How to Acknowledge us =====  ===== How to Acknowledge us ===== 
Line 14: Line 16:
 If you used our services, please acknowledge us in publications or other formal presentations. This is one of our key performance indicators and very important for future funding. You are welcome to use the following examples: If you used our services, please acknowledge us in publications or other formal presentations. This is one of our key performance indicators and very important for future funding. You are welcome to use the following examples:
  
-**Materials and Methods**: "​Microscopy experiments were performed at the Bioimaging Unit of Instituto de Medicina Molecular João Lobo Antunes, Lisboa, Portugal."+**Materials and Methods**: "​Microscopy experiments were performed at the Bioimaging Unit of Instituto de Medicina Molecular João Lobo Antunes."​
  
-**Acknowledgements**:​ "We would like to thank the technical support of the Bioimaging Unit of Instituto de Medicina Molecular João Lobo Antunes, which is supported by Portuguese ​funding PPBI-POCI-01-0145-FEDER-022122."​+**Acknowledgements**:​ "We would like to thank the Bioimaging Unit of Instituto de Medicina Molecular João Lobo Antunes ​for their technical support. We also acknowledge the funding PPBI-POCI-01-0145-FEDER-022122."​
  
 Thank you! Thank you!
Line 22: Line 24:
 ===== Services =====  ​ ===== Services =====  ​
  
-The Bioimaging Unit is equipped with 2 inverted laser point-scanning confocal microscopes prepared for live cell imaging ([[zeiss_lsm_880|Zeiss LSM 880]] and [[zeiss_lsm_710|Zeiss LSM 710]]), 1 lightsheet microscope prepared for water and clearing media immersion imaging ([[zeiss_lightsheet_z1|Zeiss Lightsheet ​Z1]]), 2 inverted spinning disk confocal microscopes with motorized stages, piezo and EM-CCDs also prepared for live cell imaging ([[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]), 1 multi-photon microscope ([[leica_sp8_mp|Leica SP8 MP]]), 3 fully motorized inverted widefield microscopes with cooled CCD cameras ([[zeiss_cell_observer|Zeiss Cell Observer]] with a large cage incubator, [[nikon_eclipse_ti|Nikon Eclipse Ti]] with stage top incubation, and [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]), 1 upright widefield fluorescence microscope with a CCD camera ([[leica_dm5000b|Leica DM5000B]]), 1 fluorescence stereo microscope with a CCD camera ([[zeiss_axiozoom_v16|Zeiss ​AxioZoom ​V16]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescent/​fluorescence imaging system ([[ivis_lumina|IVIS Lumina]]). There is also a high-spec workstation for image analysis and processing available for researchers to work on their imaging data ([[colossus|Colossus]]). The Bioimaging systems are available on the [[https://​imm.agendo.science|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience.+{{graphical_abstract_2022_dark.png?​0x400 ​ |}} The Bioimaging Unit is equipped with 2 inverted laser point-scanning confocal microscopes prepared for live cell imaging ([[zeiss_lsm_980|Zeiss LSM 980]]and [[zeiss_lsm_880|Zeiss LSM 880]], 1 lightsheet microscope prepared for water and clearing media immersion imaging ([[zeiss_lightsheet_z1|Zeiss Lightsheet ​Z.1]]), 2 inverted spinning disk confocal microscopes with motorized stages, piezo and EM-CCDs also prepared for live cell imaging ([[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]), 1 multi-photon microscope ([[leica_sp8_mp|Leica SP8 MP]]), 1 automated widefield microscope for live cell imaging ([[zeiss_celldiscoverer_7|Zeiss Celldiscoverer 7]]), 3 fully motorized inverted widefield microscopes with cooled CCD cameras ([[zeiss_cell_observer|Zeiss Cell Observer]] with a large cage incubator ​set up with an AKOYA Phenocycler fluidics system, [[nikon_eclipse_ti|Nikon Eclipse Ti]] with stage top incubation ​and a Ionoptix high-speed calcium and contractility system, and [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]), 1 fluorescence stereo microscope with a CCD camera ([[zeiss_axiozoom_v16|Zeiss ​Axio Zoom.V16]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescence ​/ fluorescence imaging system ([[ivis_lumina|IVIS Lumina]]). There is also a high-spec workstation for image analysis and processing available for researchers to work on their imaging data ([[chronos|Chronos]]). The Bioimaging systems are available on the [[https://​imm.agendo.science|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience.
  
 → **More info on our** [[resources|{{equipment_neg.png?​0x35|}}]] → **More info on our** [[resources|{{equipment_neg.png?​0x35|}}]]
Line 64: Line 66:
 === Hours === === Hours ===
  
-**9:00 - 13:00 and 14:00 - 18:00** for **Training, Assistance and Consultations**+**9:00 - 13:00 and 14:00 - 17:30** for **Training, Assistance and Consultations**
  
 24 hours, 7 days a week for Equipment Usage by **authorized,​ trained users** (see [[usage_policy|Usage Policies]]) 24 hours, 7 days a week for Equipment Usage by **authorized,​ trained users** (see [[usage_policy|Usage Policies]])
about.1644706608.txt.gz · Last modified: 2022/02/12 23:56 by bioimaging