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About the Bioimaging Unit

The Bioimaging Unit acts as a support structure to help and nurture research done with Light Microscopy inside the institute. Besides managing resources, the Bioimaging Unit provides iMM scientists and visitors with excellence in scientific know-how and expertise in using light microscopy methods for their research. We assist in planning microscopy-oriented projects, choosing materials and equipment, analyzing experimental results, processing acquired images and presenting data. Together with continuous training of new users, we organize regular courses to introduce users to the most recent microscopy techniques and foster interactions and collaborations between microscopy users at the iMM.
Photo: Jorge Figanier Castro


Head of Unit

José Rino has a Licenciatura in Technological Physics Engineering (Instituto Superior Técnico) and a PhD in Biophysics (Faculdade de Ciências). José has managed the Bioimaging Unit since January 2008 and was appointed Head in July 2009, after postdoctoral research at the iMM (Carmo-Fonseca Lab) focused on applying quantitative microscopy methods to study mRNA biogenesis. He is also Head of the Flow Cytometry Unit at iMM. Click here for more information.

Senior Technicians

António Temudo has a Licenciatura in Biology and a MSc in Evolutionary and Developmental Biology from Faculdade de Ciências, Universidade de Lisboa. António joined the Bioimaging Unit in June 2009, after studying the mechanics of somitogenesis in vertebrate embryos (mouse and zebrafish) using confocal live imaging for his MSc thesis. António has also studied Medicine at the Faculdade de Ciências Médicas, Universidade Nova de Lisboa.

Ana Nascimento has a Licenciatura in Chemistry and a MSc in Biochemistry from Faculdade de Ciências, Universidade de Lisboa. Ana joined the Bioimaging Unit in December 2012 after working as a flow cytometry technician and a microscopy technician at Instituto Gulbenkian de Ciência.


Aida Lima has an Undergraduate degree in Biology and a MSc in Genetic Molecular Biology and Biomedicine from Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa. Aida joined the Bioimaging Unit in June 2019, after studying the dynamics of the actin cytoskeleton in endothelial tip cells using confocal microscopy, in Cláudio Franco's lab at the iMM.

Clara Barreto has an Undergraduate degree in Biology and a MSc in Molecular Biology and Genetics from Faculdade de Ciências, Universidade de Lisboa. Clara joined the Bioimaging Unit in January 2020 after working at the Electron Microscopy Facility at Instituto Gulbenkian de Ciência and at the Instituto de Biologia Experimental e Tecnológica, developing ImageJ macros for automated image analysis of breast cancer tissue.


The Bioimaging Unit is equipped with 2 inverted laser point-scanning confocal microscopes prepared for live cell imaging (Zeiss LSM 880 and Zeiss LSM 710), 1 lightsheet microscope prepared for water and clearing media immersion imaging (Zeiss Lightsheet Z1), 2 inverted spinning disk confocal microscopes with motorized stage, piezo and EM-CCD also prepared for live cell imaging (3i Marianas SDC and Zeiss Cell Observer SD), 1 multi-photon microscope (Leica SP8 MP), 4 fully motorized inverted widefield microscopes with cooled CCD cameras and incubators (Zeiss Cell Observer, Zeiss Axiovert 200M, Nikon Eclipse Ti and Nikon Eclipse TE-2000-E), 1 upright widefield fluorescence microscope with a CCD camera (Leica DM5000B), 2 fluorescence stereo microscopes with cooled CCD cameras (Zeiss Lumar V12 and Zeiss AxioZoom V16) and other imaging resources such as a brightfield microscope (Leica DM2500) and a bioluminescent imaging system (IVIS Lumina). We also have two high-spec computers for image processing available for researchers to work on their imaging data (Big Guy and Colossus). The Bioimaging systems are available on the online booking system for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience.

More info on our


We charge according to booked time in each microscopy system. Online booking is mandatory for each usage session and cancellations are allowed if they are made prior to reserved time. Users that do not show up at their booked sessions will be charged in full.

Check our


9 a.m. - 6 p.m. for Training, Assistance and Consultations

24 hours, 7 days a week for Equipment Usage by authorized, trained users (see Usage Rules)

How to use the service

All new users must contact the Bioimaging Unit for training in each specific system. This also applies to researchers with previous experience in microscopy. Only trained users are allowed to book usage sessions on a particular microscope.

Are you a


Bioimaging Unit

Instituto de Medicina Molecular, room P2-A-32
Faculdade de Medicina de Lisboa
Av. Prof. Egas Moniz
1649-028 Lisboa

Photo: Bioimaging Unit in 2016 by Ana de Barros

Internal phone extension: 47222

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about.1587509317.txt.gz · Last modified: 2020/04/22 00:48 by bioimaging