about
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| ====== About the Bioimaging Unit ====== | ====== About the Bioimaging Unit ====== | ||
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| - | {{::team_photo_2020.jpg?600 |}}The Bioimaging Unit acts as a support structure to help and nurture research done with Light Microscopy inside the institute. Besides managing resources, the Bioimaging Unit provides iMM scientists and visitors with excellence in scientific know-how and expertise in using light microscopy methods for their research. We assist in planning microscopy-oriented projects, choosing materials and equipment, analyzing experimental results, processing acquired images and presenting data. Together with continuous training of new users, we organize regular courses to introduce users to the most recent microscopy techniques and foster interactions and collaborations between microscopy users at the iMM. | ||
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| - | ===== Team ===== | ||
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| - | <table style="border:0px solid white;"> | + | <iframe width="560" height="315" src="https://www.youtube.com/embed/9ntTKoIexF4" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe> |
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| - | <div style="text-align: center;"><b>Head of Unit</b></div> | + | |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/bioimaging/lib/exe/fetch.php?media=rino.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>José Rino</b> has a <i>Licenciatura</i> in Technological Physics Engineering (Instituto Superior Técnico) and a PhD in Biophysics (Faculdade de Ciências). José has managed the Bioimaging Unit since January 2008 and was appointed Head in July 2009, after postdoctoral research at the iMM (Carmo-Fonseca Lab) focused on applying quantitative microscopy methods to study mRNA biogenesis. He is also Head of the Flow Cytometry Unit at iMM. <a href="https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=facility_bioimaging_doku.php&id=rino_cv">Click here for more information</a>.<br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>joserino@medicina.ulisboa.pt</i> | + | |
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| + | [[team|{{ ::ubi_team_2022.jpeg?0x300|}}]]The Bioimaging Unit acts as a support structure to help and nurture research done with Light Microscopy. The people we work with have amazing questions to answer. But if used incorrectly, a microscope can give you wrong answers. At the Bioimaging Unit we focus on people and their questions. We provide iMM scientists and visitors with excellence in scientific know-how and expertise in light microscopy methods, in a personalized approach. We sit with researchers to teach them, train them, create software tools for them and help them make the most out of the microscope. We provide support in all steps of research done with Light Microscopy. We help researchers get to that moment when something is seen for the first time. To see Science done right, see it happening. That’s unique. | ||
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| + | → **Meet the** [[team|{{team_neg.png?0x35|}}]] | ||
| - | <html> | + | We are a node of the [[https://www.ppbi.pt/joomla30/index.php/ppbi|Portuguese Platform of Bioimaging (PPBI)]], a nationwide consortium of imaging facilities which integrates the European research infrastructure as a Biological Node of [[https://www.eurobioimaging.eu/|Euro-BioImaging ERIC]]. We are also a [[https://www.zeiss.com/microscopy/int/about-us/microscopy-customer-center/labs-location-partner.html|Zeiss labs@location Partner]], integrating a community of advanced microscopy facilities providing in-depth knowledge, dedicated services across a wide range of applications and instrumentation, and scientific support to ZEISS customers. |
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| - | <div align="center"><b>Senior Technicians</b></div> | + | |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/bioimaging/lib/exe/fetch.php?media=temudo.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>António Temudo</b> has a <i>Licenciatura</i> in Biology and a MSc in Evolutionary and Developmental Biology from Faculdade de Ciências, Universidade de Lisboa. António joined the Bioimaging Unit in June 2009, after studying the mechanics of somitogenesis in vertebrate embryos (mouse and zebrafish) using confocal live imaging for his MSc thesis. António has also studied Medicine at the Faculdade de Ciências Médicas, Universidade Nova de Lisboa.<br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>atemudo@medicina.ulisboa.pt</i> | + | |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nascimento.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>Ana Nascimento</b> has a <i>Licenciatura</i> in Chemistry and a MSc in Biochemistry from Faculdade de Ciências, Universidade de Lisboa. Ana joined the Bioimaging Unit in December 2012 after working as a flow cytometry technician and a microscopy technician at Instituto Gulbenkian de Ciência. <br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>anascimento@medicina.ulisboa.pt</i> | + | |
| - | </td> | + | |
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| - | <html> | + | ===== How to Acknowledge us ===== |
| - | <table style="border:0px solid white;"> | + | |
| - | <tr style="border:0px solid white;"> | + | If you used our services, please acknowledge us in publications or other formal presentations. This is one of our key performance indicators and very important for future funding. You are welcome to use the following examples: |
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| - | <div align="center"><b>Technicians</b></div> | + | **Materials and Methods**: "Microscopy experiments were performed at the Bioimaging Unit of Instituto de Medicina Molecular João Lobo Antunes." |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/bioimaging/lib/exe/fetch.php?media=lima.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>Aida Lima</b> has an Undergraduate degree in Biology and a MSc in Genetic Molecular Biology and Biomedicine from Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa. Aida joined the Bioimaging Unit in June 2019, after studying the dynamics of the actin cytoskeleton in endothelial tip cells using confocal microscopy, in Cláudio Franco's lab at the iMM. <br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>aidalima@medicina.ulisboa.pt</i> | + | |
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| - | <div style="width: 150px; height: 150px; overflow: hidden; position: relative;"><img src="/facility/bioimaging/lib/exe/fetch.php?media=barreto.png" style="width:150px; height: 150px;" /></div><br><br> | + | |
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| - | <b>Clara Barreto</b> has an Undergraduate degree in Biology and a MSc in Molecular Biology and Genetics from Faculdade de Ciências, Universidade de Lisboa. Clara joined the Bioimaging Unit in January 2020 after working at the Electron Microscopy Facility at Instituto Gulbenkian de Ciência and at the Instituto de Biologia Experimental e Tecnológica, developing ImageJ macros for automated image analysis of breast cancer tissue. <br> | + | |
| - | <img src="https://imm.medicina.ulisboa.pt/facility/flowcytometry/lib/exe/fetch.php?media=mail_icon.png" width=20> | + | |
| - | <i>clara.barreto@medicina.ulisboa.pt</i> | + | |
| - | </td> | + | |
| - | </tr> | + | |
| - | </table> | + | |
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| + | **Acknowledgements**: "We would like to thank the Bioimaging Unit of Instituto de Medicina Molecular João Lobo Antunes for their technical support. We also acknowledge the funding PPBI-POCI-01-0145-FEDER-022122." | ||
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| + | Thank you! | ||
| ===== Services ===== | ===== Services ===== | ||
| - | The Bioimaging Unit is equipped with 2 inverted laser point-scanning confocal microscopes prepared for live cell imaging ([[zeiss_lsm_880|Zeiss LSM 880]] and [[zeiss_lsm_710|Zeiss LSM 710]]), 1 lightsheet microscope prepared for water and clearing media immersion imaging ([[zeiss_lightsheet_z1|Zeiss Lightsheet Z1]]), 2 inverted spinning disk confocal microscopes with motorized stage, piezo and EM-CCD also prepared for live cell imaging ([[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]), 1 multi-photon microscope ([[leica_sp8_mp|Leica SP8 MP]]), 4 fully motorized inverted widefield microscopes with cooled CCD cameras and incubators ([[zeiss_cell_observer|Zeiss Cell Observer]], [[zeiss_axiovert_200m|Zeiss Axiovert 200M]], [[nikon_eclipse_ti|Nikon Eclipse Ti]] and [[nikon_eclipse_te2000e|Nikon Eclipse TE-2000-E]]), 1 upright widefield fluorescence microscope with a CCD camera ([[leica_dm5000b|Leica DM5000B]]), 2 fluorescence stereo microscopes with cooled CCD cameras ([[zeiss_lumar_v12|Zeiss Lumar V12]] and [[zeiss_axiozoom_v16|Zeiss AxioZoom V16]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescent imaging system ([[ivis_lumina|IVIS Lumina]]). We also have two high-spec computers for image processing available for researchers to work on their imaging data ([[big_guy|Big Guy]] and [[colossus|Colossus]]). The Bioimaging systems are available on the [[https://imm.medicina.ulisboa.pt/intranet/booking/select_resource.php?v=1|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience. | + | {{graphical_abstract_2022_dark.png?0x400 |}} The Bioimaging Unit is equipped with 2 inverted laser point-scanning confocal microscopes prepared for live cell imaging ([[zeiss_lsm_980|Zeiss LSM 980]], and [[zeiss_lsm_880|Zeiss LSM 880]], 1 lightsheet microscope prepared for water and clearing media immersion imaging ([[zeiss_lightsheet_z1|Zeiss Lightsheet Z.1]]), 2 inverted spinning disk confocal microscopes with motorized stages, piezo and EM-CCDs also prepared for live cell imaging ([[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]), 1 multi-photon microscope ([[leica_sp8_mp|Leica SP8 MP]]), 1 automated widefield microscope for live cell imaging ([[zeiss_celldiscoverer_7|Zeiss Celldiscoverer 7]]), 3 fully motorized inverted widefield microscopes with cooled CCD cameras ([[zeiss_cell_observer|Zeiss Cell Observer]] with a large cage incubator set up with an AKOYA Phenocycler fluidics system, [[nikon_eclipse_ti|Nikon Eclipse Ti]] with stage top incubation and a Ionoptix high-speed calcium and contractility system, and [[zeiss_axiovert_200m|Zeiss Axiovert 200M]]), 1 fluorescence stereo microscope with a CCD camera ([[zeiss_axiozoom_v16|Zeiss Axio Zoom.V16]]) and other imaging resources such as a brightfield microscope ([[leica_dm2500|Leica DM2500]]) and a bioluminescence / fluorescence imaging system ([[ivis_lumina|IVIS Lumina]]). There is also a high-spec workstation for image analysis and processing available for researchers to work on their imaging data ([[chronos|Chronos]]). The Bioimaging systems are available on the [[https://imm.agendo.science|online booking system]] for trained researchers 7 days a week and 24 hours per day. User training in each particular system is mandatory, even for researchers with previous microscopy experience. |
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| + | → **More info on our** [[resources|{{equipment_neg.png?0x35|}}]] | ||
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| + | The Bioimaging Unit provides support in all steps of research done with Light Microscopy at the iMM, from the first ideas in Project Planning to the final Data Publication, with a strong focus in Microscope Training and Support and Image Analysis and Processing. | ||
| - | → **More info on our** [[resources|{{resources_neg.png?0x35|}}]] | ||
| {{process_en_dark.png|}} | {{process_en_dark.png|}} | ||
| ===== Prices ===== | ===== Prices ===== | ||
| - | We charge according to booked time in each microscopy system. [[https://imm.medicina.ulisboa.pt/intranet/booking/select_resource.php?v=1|Online booking]] is mandatory for each usage session and cancellations are allowed if they are made prior to reserved time. Users that do not show up at their booked sessions will be charged in full. | + | We charge according to booked time in each microscopy system. [[https://imm.agendo.science/|Online booking]] is mandatory for each usage session and cancellations are allowed if they are made prior to reserved time. Users that do not show up at their booked sessions will be charged in full. |
| → **Check our** [[billing|{{charge_rates_neg.png?0x35|}}]] | → **Check our** [[billing|{{charge_rates_neg.png?0x35|}}]] | ||
| - | ===== Hours ===== | ||
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| - | **9 a.m. - 6 p.m.** for **Training, Assistance and Consultations** | ||
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| - | 24 hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Rules]]) | ||
| ===== How to use the service ===== | ===== How to use the service ===== | ||
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| All new users must contact the Bioimaging Unit for training in each specific system. This also applies to researchers with previous experience in microscopy. Only trained users are allowed to book usage sessions on a particular microscope. | All new users must contact the Bioimaging Unit for training in each specific system. This also applies to researchers with previous experience in microscopy. Only trained users are allowed to book usage sessions on a particular microscope. | ||
| - | {{:users_over_years_neg.png?400|}} {{:usage_hours_neg.png?450|}} \\ | + | → **How to** [[newuser|{{new_user_neg.png?0x35|}}]] |
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| - | → **Are you a** [[newuser|{{new_user_neg.png?0x35|}}]] | + | |
| ===== Contacts ===== | ===== Contacts ===== | ||
| - | {{:bioimaging_team.jpg?400|}} | + | {{:bioimaging_team.jpg?400 |}}Bioimaging Unit \\ |
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| - | Bioimaging Unit \\ | + | |
| **imm-bioimaging@medicina.ulisboa.pt** | **imm-bioimaging@medicina.ulisboa.pt** | ||
| - | Instituto de Medicina Molecular, room P2-A-32 \\ | + | Instituto de Medicina Molecular, room P3-A-5 \\ |
| Faculdade de Medicina de Lisboa \\ | Faculdade de Medicina de Lisboa \\ | ||
| Av. Prof. Egas Moniz \\ | Av. Prof. Egas Moniz \\ | ||
| 1649-028 Lisboa \\ | 1649-028 Lisboa \\ | ||
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| + | Photo: Bioimaging Unit in 2016 by [[https://anadebarros.github.io/|Ana de Barros]] | ||
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| + | === Hours === | ||
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| + | **9:00 - 13:00 and 14:00 - 17:30** for **Training, Assistance and Consultations** | ||
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| + | 24 hours, 7 days a week for Equipment Usage by **authorized, trained users** (see [[usage_policy|Usage Policies]]) | ||
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| {{bioimaging_map_new.png|}} | {{bioimaging_map_new.png|}} | ||
| - | {{phone_neg.png?20|}} Internal phone extension: 47222 | + | {{phone_neg.png?20|}} Internal phone extension: 47305 |
| ---- | ---- | ||
| [[start|Back to the Bioimaging Wiki]] | [[start|Back to the Bioimaging Wiki]] | ||
about.txt · Last modified: 2024/01/28 13:58 by bioimaging
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