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3i_marianas_sdc [2020/02/28 00:40]
bioimaging [System overview]
3i_marianas_sdc [2022/02/14 22:14] (current)
bioimaging [System overview]
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 <​b>​Model</​b>:​ <a href="​https://​www.intelligent-imaging.com/​marianas"​ target="​_blank">​Marianas</​a><​br>​ <​b>​Model</​b>:​ <a href="​https://​www.intelligent-imaging.com/​marianas"​ target="​_blank">​Marianas</​a><​br>​
 <​b>​Nickname</​b>:​ "​Marianas"<​br>​ <​b>​Nickname</​b>:​ "​Marianas"<​br>​
-<​b>​Software</​b>:​ SlideBook 6.0.4<br>+<​b>​Software</​b>:​ SlideBook 6.0.22<br>
 <​b>​Year</​b>:​ 2012 <br> <​b>​Year</​b>:​ 2012 <br>
 <​b>​SN</​b>:​ 1648 <br> <​b>​SN</​b>:​ 1648 <br>
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 &nbsp <br> &nbsp <br>
 &nbsp <br> &nbsp <br>
-&rarr; <a href="​https://​imm.medicina.ulisboa.pt/​intranet/booking/111"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ 3i Marianas SDC Booking</​a>​+&rarr; <a href="​https://​my.agendo.science/calendar/?​id=MjdjYWxlbmRhcnM="><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=date.png"> ​ 3i Marianas SDC Booking</​a>​
  <​br> ​  <​br> ​
 &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=3i_marianas_sdc_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ 3i Marianas SDC Quality Control</​a>​ &rarr; <a href="/​facility/​bioimaging/​doku.php?​id=3i_marianas_sdc_quality"><​img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=list-icon.png"​ width=18>​ 3i Marianas SDC Quality Control</​a>​
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-{{  :spinning_disk.png|}} The 3i Marianas SDC //spinning disk confocal microscope//​ is a fast imaging system which provides a trade-off between confocality,​ resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator ​for temperature control ​and a small stage incubator for CO2 supply. With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]]. Even though its resolution is not as high as a //​point-scanning confocal system// such as the [[zeiss_lsm_710|Zeiss LSM 710]], it is much faster. You can also check the [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]] if you need a spinning disk with Definite Focus system ​for long time-lapse acquisitions. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. ​+{{  :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope//​ is a fast imaging system which provides a trade-off between confocality,​ resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the [[zeiss_cell_observer|Zeiss Cell Observer]]. Even though its resolution is not as high as a //​point-scanning confocal system// such as the [[zeiss_lsm_710|Zeiss LSM 710]], it is much faster. You can also check the [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]] if you need a spinning disk with Definite Focus for large tiling or long time-lapse acquisitions. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. ​
  
   * **Microscope**:​ [[http://​www.zeiss.com/​microscopy/​en_de/​products/​light-microscopes/​axio-observer-for-biology.html|Zeiss Axio Observer]]   * **Microscope**:​ [[http://​www.zeiss.com/​microscopy/​en_de/​products/​light-microscopes/​axio-observer-for-biology.html|Zeiss Axio Observer]]
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 ^  Model  ^  Frame Size  ^  Pixel Size (µm)  ^  Quantum Efficiency ​ ^ ^  Model  ^  Frame Size  ^  Pixel Size (µm)  ^  Quantum Efficiency ​ ^
 |  [[https://​www.photometrics.com/​wp-content/​uploads/​2019/​10/​Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] ​ |  512 x 512  |  16 x 16  |  > 90%  | |  [[https://​www.photometrics.com/​wp-content/​uploads/​2019/​10/​Evolve512-Datasheet.pdf|Photometrics Evolve 512 EMCCD]] ​ |  512 x 512  |  16 x 16  |  > 90%  |
 +
 ===== Microscope Turn On Procedure ===== ===== Microscope Turn On Procedure =====
   * Turn on the fluorescence lamp (if needed)   * Turn on the fluorescence lamp (if needed)
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   * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** from left to right   * Turn on the power switch distributor (green) and the component power switches (red) **one at a time** from left to right
 {{marianas_power3.jpg?​|}} {{marianas_power3.jpg?​|}}
-  * Turn on the secondary power supply switch (white socket outlet underneath the touchscreen) +  * Turn on the secondary power supply switch (socket outlet underneath the touchscreen) 
-  * Turn on incubation and CO2 on the touchscreen (if needed)+{{::​marianas_power2.png|}} 
 +  * Turn on incubation and CO2 in the touchscreen (if needed)
 {{marianas_power6.jpg?​|}} {{marianas_power6.jpg?​|}}
   * Turn on the lasers you need to use (using the key and the power switches)   * Turn on the lasers you need to use (using the key and the power switches)
-{{marianas_power7.jpg?|}}+{{::marianas_power7.png?160|}}
   * Turn on the computer   * Turn on the computer
 +  * Login in to Windows (Bioimaging User)
   * Wait 2 min to allow for system components detection   * Wait 2 min to allow for system components detection
   * Start the **SlideBook** software   * Start the **SlideBook** software
- 
-**Warning**:​ If you need to change the stage adaptor, please contact the <​html><​a href="/​facility/​bioimaging/​doku.php?​id=about">​Bioimaging Unit</​a>​ (<​b>​imm-bioimaging@medicina.ulisboa.pt</​b>​ | <img src="/​facility/​bioimaging/​lib/​exe/​fetch.php?​media=phone_neg.png"​ width=15>​ 47222</​html>​) 
  
 ===== Microscope Turn Off Procedure ===== ===== Microscope Turn Off Procedure =====
-  ​Remove your sample from the stage/piezo holder+If there is another user for this microscope in the __next hour__: 
 +  ​Exit the SlideBook software, leave the lasers on 
 +  * Log off the computer 
 +  * Clean up immersion objectives 
 +Else:
   * Exit the **SlideBook** software   * Exit the **SlideBook** software
-  * Switch off the computer+  * Shut down the computer
   * Turn off incubation in the touchscreen (if used)   * Turn off incubation in the touchscreen (if used)
   * Turn off CO2 (if used)   * Turn off CO2 (if used)
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 ---- ----
  
-[[resources|Back to the Resources ​page]]+[[resources|Back to the Equipment ​page]]
3i_marianas_sdc.1582846802.txt.gz · Last modified: 2020/02/28 00:40 by bioimaging