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3i_marianas_sdc

Location: Room P2-A-22 ( 47217)
Manufacturer: Intelligent Imaging Innovations
Model: Marianas
Nickname: "Marianas"
Software: SlideBook 6.0.4
Year: 2012
SN: 1648
 
 
 
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System overview

The 3i Marianas SDC spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512×512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control and a small stage incubator for CO2 supply. With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the Zeiss Cell Observer. Even though its resolution is not as high as a point-scanning confocal system such as the Zeiss LSM 880 or Zeiss LSM 710, it is much faster than these two systems. You can also check the Zeiss Cell Observer SD if you need a spinning disk with a Definite Focus system for long time-lapse acquisitions. If your personal computer cannot handle all the data you collected, check out the Big Guy or Colossus.

Data files older than 1 month will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

System components

LASERs

Laser Unit Wavelength Maximum Power Current Status
Laserstack 405 405 nm 100 mW ok
Laserstack 488 488 nm 100 mW ok
Laserstack 561 561 nm 100 mW ok
Laserstack 640 640 nm 100 mW ok

Objectives

Magnification Model Immersion NA WD (mm) Reference
20x Plan-Apochromat Air 0.8 0.55 420650-9902-000
63x Plan-Apochromat Oil 1.40 0.19 420780-9900-000
100x Plan-Apochromat Oil 1.40 0.17 420790-9901-000

Upon request:

Magnification Model Immersion NA WD (mm) Reference
25x LCI Plan-Neofluar Corr DIC Oil/Glyc/W 0.80 0.21 420852-9972-000
40x Plan-Apochromat Corr Air 0.95 0.25 420660-9970-000

Emission Filters

Setting Transmission
1: 445 422-477 nm
2: 525 510-540 nm
3: 617 580-653 nm
4: 692 672-712 nm
5: QUAD 440+521+607+700 nm
6: Block No Transmission
7: Block No Transmission
8: Block No Transmission
9: Empty Empty
10: 445 422-477 nm

Camera

Model Frame Size Pixel Size (µm) Quantum Efficiency
Photometrics Evolve 512 EMCCD 512 x 512 16 x 16 > 90%

Microscope Turn On Procedure

  • Turn on the fluorescence lamp (if needed)

  • Turn on the power switch distributor (green) and the component power switches (red) one at a time from left to right

  • Turn on the secondary power supply switch (white socket outlet underneath the touchscreen)
  • Turn on incubation and CO2 on the touchscreen (if needed)

  • Turn on the lasers you need to use (using the key and the power switches)

  • Turn on the computer
  • Wait 2 min to allow for system components detection
  • Start the SlideBook software

Warning: If you need to change the stage adaptor, please contact the Bioimaging Unit (imm-bioimaging@medicina.ulisboa.pt | 47222)

Microscope Turn Off Procedure

  • Remove your sample from the stage/piezo holder
  • Exit the SlideBook software
  • Switch off the computer
  • Turn off incubation in the touchscreen (if used)
  • Turn off CO2 (if used)
  • Switch all lasers off (first on the switch and finally by turning the key)
  • Turn off the fluorescent lamp
  • Turn off the secondary power supply switch (white socket outlet underneath the touchscreen)
  • Turn off the component power switches (red) one at a time from right to left and the power switch distributor (green)

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3i_marianas_sdc.txt · Last modified: 2020/02/17 11:45 by bioimaging