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Location: Room P2-A-22 ( 47217)
Manufacturer: Intelligent Imaging Innovations
Model: Marianas
Nickname: "Marianas"
Software: SlideBook 6.0.22
Year: 2012
SN: 1648
3i Marianas SDC Quality Control
3i Marianas SDC Usage Statistics  

System overview

The 3i Marianas SDC spinning disk confocal microscope is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512×512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large cage incubator and a stage top incubator for temperature control and CO2 supply. With this system you can perform optical sectioning of fluorescent samples which are too thick for a widefield system such as the Zeiss Cell Observer. Even though its resolution is not as high as a point-scanning confocal system such as the Zeiss LSM 710, it is much faster. You can also check the Zeiss Cell Observer SD if you need a spinning disk with Definite Focus 2 for large tiling or long time-lapse acquisitions. If your personal computer cannot handle all the data you collected, check out the Colossus.

Data files older than 15 days will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.

System components


Laser Unit Wavelength Maximum Power
Laserstack 405 405 nm 100 mW
Laserstack 488 488 nm 100 mW
Laserstack 561 561 nm 100 mW
Laserstack 640 640 nm 100 mW


Magnification Model Immersion NA WD (mm) Reference
10x EC Plan-Neofluar Air 0.30 5.50 420340-9900-000
20x Plan-Apochromat Air 0.80 0.55 420650-9901-000
40x LD C-Apochromat Corr Water 1.10 0.62 421867-9970-000
63x Plan-Apochromat Oil 1.40 0.19 420780-9900-000
100x Plan-Apochromat Oil 1.40 0.17 420790-9901-000

Upon request:

Magnification Model Immersion NA WD (mm) Reference
25x LCI Plan-Neofluar Corr DIC Oil/Glyc/W 0.80 0.21 420852-9972-000
40x Plan-Apochromat Corr Air 0.95 0.25 420660-9970-000

Emission Filters

Setting Transmission
1: 445 422-477 nm
2: 525 510-540 nm
3: 617 580-653 nm
4: 692 672-712 nm
5: QUAD 440+521+607+700 nm
6: Block No Transmission
7: Block No Transmission
8: Block No Transmission
9: Empty Empty
10: 445 422-477 nm


Model Frame Size Pixel Size (µm) Quantum Efficiency
Photometrics Evolve 512 EMCCD 512 x 512 16 x 16 > 90%

Microscope Turn On Procedure

  • Turn on the fluorescence lamp (if needed)

  • Turn on the power switch distributor (green) and the component power switches (red) one at a time from left to right

  • Turn on the secondary power supply switch (socket outlet underneath the touchscreen)

  • Turn on incubation and CO2 in the touchscreen (if needed)

  • Turn on the lasers you need to use (using the key and the power switches)

  • Turn on the computer
  • Login in to Windows with your Agendo credentials
  • Wait 2 min to allow for system components detection
  • Start the SlideBook software

Microscope Turn Off Procedure

If there is another user for this microscope in the next hour:

  • Exit the SlideBook software, leave the lasers on
  • Log off the computer
  • Clean up immersion objectives


  • Exit the SlideBook software
  • Shut down the computer
  • Turn off incubation in the touchscreen (if used)
  • Turn off CO2 (if used)
  • Switch all lasers off (first on the switch and finally by turning the key)
  • Turn off the fluorescent lamp
  • Turn off the secondary power supply switch (white socket outlet underneath the touchscreen)
  • Turn off the component power switches (red) one at a time from right to left and the power switch distributor (green)

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3i_marianas_sdc.txt · Last modified: 2022/09/02 02:13 by bioimaging