Table of Contents
Zeiss LSM 510 (no longer available)
Zeiss LSM 510 Booking
Zeiss LSM 510 Usage Report
Location: no longer available
Manufacturer: Zeiss
Model: LSM 510
Year: 1998
SN: 450240
Microscope overview
The LSM 510 system is a point scanning laser confocal microscope prepared for live imaging and photobleaching experiments. The system can be equipped with a small size incubator for temperature control.
LASER units
| Laser Unit | Wavelength | Maximum Power | Current Status |
|---|---|---|---|
| Argon | 458, 488 and 514 nm | 25 mW | not working |
| HeNe1 | 543 nm | 1 mW | not working |
| HeNe2 | 633 nm | 5 mW | ok |
Objectives
| Magnification | Class | Immersion | NA | More info |
|---|---|---|---|---|
| 10x | Plan-Neofluar | No | 0.30 | 440330-0000 |
| 63x | Plan-Apochromat | Oil | 1.40 | 440761-9905 |
| 100x | Plan-Neofluar | Oil | 1.30 | 440480-9903 |
Filter and Dichroic sets
 |  |  |  |  |
| Main configuration window | Emission filters for channel 1 | Emission filters for channel 2 | Channel separation dichroic | Laser separation dichroic |
Microscope Turn On Procedures
- Turn on the two UPS near the microscope.
- Turn the remote control on.
- Turn the HBO lamp on.
- Turn the halogen transmitted light power source on.
- Turn the computer on.
- Login to the computer, start the LSM software.
Microscope Turn Off procedures
If there's another user for this microscope in the next two hours:
- Close the software, leave the lasers on an log off the computer.
- Clean up any objective were you used immersion oil.
- Make sure that there really is another user going to use the microscope.
Else:
- Clean up any objective were you used immersion oil.
- Turn off the lasers in the software (wait for laser cooling!)
- Clean up immersion objectives
- Turn off the HBO lamp.
- Turn off the transmitted light lamp.
- Shutdown the computer.
- Turn off the remote control.
- Turn off the two ups.
External links
- This PDF shows how to work with the system, it's not exactly the same system configuration but teaches a lot about the software.