Table of Contents
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Location: Room P2-A-24 ( |
47219) 
Zeiss Axiovert 200M Quality Control
Zeiss Axiovert 200M Usage Statistics
Microscope overview
The Zeiss Axiovert 200M is a fully motorized inverted widefield fluorescence microscope ideal for live-cell imaging applications. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Photometrics CoolSNAP HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. It is also equipped with a color camera (Leica DFC450) for acquisition of histological stains with tile and stitching imaging.
- Microscope: Zeiss Axiovert 200
- Monochrome Camera: Photometrics Coolsnap HQ CCD
- Color Camera: Leica DFC450
Data files older than 1 month will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
System components
Filtersets
| Position | Filterset | Reference | Excitation | Dichroic | Emission |
|---|---|---|---|---|---|
| 1 | Blue | FS01 | 359-371 nm | 395 nm | > 397 nm |
| 2 | Green BP | FS10 | 450-490 nm | 510 nm | 515-565 nm |
| 3 | Red | FS20 | 540-552 nm | 560 nm | 575-640 nm |
| 4 | Far Red | Cy5-4040C-000 | 608-648 nm | 660 nm | 672-712 nm |
| 5 | A | none | |||
Upon request:
| Position | Filterset | Reference | Excitation | Dichroic | Emission |
|---|---|---|---|---|---|
| 2 | Green LP | FS09 | 450-490 nm | 510 nm | > 515 nm |
Objectives
| Magnification | Model | Immersion | NA | WD (mm) | pixel size* (μm) | 10 μm = | (color) 10 μm = | Reference |
|---|---|---|---|---|---|---|---|---|
| 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | 1.00 | 10 pixels | 23.3 pixels | 420340-9900-000 |
| 20x | EC Plan-Neofluar | Air | 0.50 | 2.00 | 0.48 | 21 pixels | 46.7 pixels | 440340-9904-000 |
| 40x | EC Plan-NeoFluar | Air | 0.75 | 0.71 | 0.24 | 41 pixels | 93.7 pixels | 440350-9903-000 |
| 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | 0.15 | 65 pixels | - | 420782-9900-000 |
| 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | 0.09 | 105 pixels | - | 420792-9800-000 |
Upon request:
| Magnification | Model | Immersion | NA | WD (mm) | pixel size* (μm) | 10 μm = | Reference |
|---|---|---|---|---|---|---|---|
| 1.25x | EC Epiplan-Neofluar | Air | 0.035 | 4 | 7.58 | 1.3 pixels | 422310-9900-000 |
| 5x | EC Plan-NeoFluar | Air | 0.16 | 18.5 | 2.00 | 5 pixels | 420330-9901-000 |
| 40x | Plan-Neofluar Ph3 | Oil | 1.30 | 0.20 | 0.24 | 41 pixels | 440451-9903-000 |
| 63x | Plan-Apochromat Ph3 | Oil | 1.4 | 0.19 | 0.15 | 65 pixels | 420781-9910-000 |
* pixel sizes for binning = 1
Cameras
| Model | Type | Frame Size | Pixel Size (µm) |
|---|---|---|---|
| Photometrics Coolsnap HQ | CCD | 1392 x 1040 | 6.45 x 6.45 |
| Leica DFC450 | color CCD | 2560 × 1920 | 3.4 x 3.4 |
Stage
For automated acquisition of 96 well plates use: X = -9014 μm Y = -8995 μm
System Turn On Procedures
- Turn on the fluorescent light power source (if used)
- Turn on the Prior stage controller
- Turn on the microscope
- Turn on the CCD camera
- Turn on the computer
- Log in to Windows (Bioimaging User)
- Start the Metamorph software
- If the software doesn't detect the camera, power cycle the camera and try again
Microscope Turn Off procedures
If there is another user for this microscope in the next hour:
- Leave the fluorescent lamp on (if used)
- Clean up immersion objectives
Else:
- Clean up immersion objectives
- Shutdown the computer
- Turn off the camera
- Turn off the Prior stage controller
- Turn off the microscope
- Turn off the fluorescent lamp (if used)