Image by Maria Henriques, João Ferreira and José Rino PSFs
We image sub-resolution fluorescent beads (0.17 μm diameter) and use PSFj to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.

Illumination
We measure the intensity of laser lines, LEDs and fluorescent lamps for each system.


Spinning Disc Confocal Microscopes

Point Scanning Confocal Microscopes

Multi-photon Microscope

Widefield Fluorescence Microscopes

Fluorescence Stereo Microscope

Other Imaging Systems


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