Table of Contents
|
Location: [Oeiras] Room 0B03 |
ZEISS Stereo Lumra.V12 Usage StatisticsMicroscope overview
The Stereo Lumar V.12 is a motorized stereoscope with fluorescence, capable of imaging in colour and grayscale (camera dependent), with a zoom between 9.6x and 120x.
System components
| Position | Filterset | Reference | Excitation | Dichroic | Emission |
|---|---|---|---|---|---|
| 1 | CFP | Filterset 47 | 436/20 | 455 | 480/40 |
| 2 | GFP | Filterset 13 | 470/20 | 495 | 505-530 |
| 3 | TexasRed | Filterset 45 | 560/40 | 585 | 630/75 |
| 4 | YFP | Filterset 46 | 500/20 | 515 | 535/30 |
Camera
Camera can be changed on request, and training for this can be provided if needed.
| Model | Frame Size | Pixel Size (µm) | Quantum Efficiency |
|---|---|---|---|
| UI-3370CP Rev. 2 Colour | 2048 × 2048 | 5.5 x 5.5 | - |
| ORCA-Flash4.0 LT+ Monochrome | 2048 × 2048 | 6.5 x 6.5 | 82% |
Pixel-size vs magnification
| Magnification | Hamamatsu (µm/pixel) | IDS (µm/pixel) |
|---|---|---|
| 9.6x | 10.90 | 9.40 |
| 10.0x | 10.49 | 9.02 |
| 20.0x | 5.07 | 4.52 |
| 30.0x | 3.41 | 3.02 |
| 40.0x | 2.59 | 2.26 |
| 50.0x | 2.05 | 1.81 |
| 60.0x | 1.73 | 1.51 |
| 70.0x | 1.49 | 1.30 |
| 80.0x | 1.29 | 1.13 |
| 90.0x | 1.16 | 1.01 |
| 100.0x | 1.04 | 0.91 |
| 110.0x | 0.95 | 0.83 |
| 120.0x | 0.87 | 0.76 |
Microscope Turn On Procedure
- Turn on the powerbar below the stone table.
- If fluorescence is necessary, turn on the fluorescent lamp controller on top of the desk.
Microscope Turn Off Procedure
- Turn on the powerbar below the stone table.
- If fluorescence was used, turn off the fluorescent lamp controller on top of the desk.
- This lamp has to be on for 30 minutes. If you use the system less than 30 minutes, wait until that time to turn the lamp off, or warn the Bioimaging Team.