Table of Contents

Location: [Oeiras] Room 0B04
Manufacturer: Intelligent Imaging Innovations
Model: Marianas SDC
Nickname: "Marianas"
Software: SlideBook 2024
Year: 2021
SN:
 
Data will be deleted after: 1 month  
 
→ 3i Marianas SDC Quality Control
→ 3i Marianas SDC Usage Statistics

Microscope overview

The 3i Marianas SDC spinning disk confocal microscope is a fast imaging system which provides a combination of confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and specimen to generate point-like illumination covering the whole area “simultaneously”. Each small aperture serves as a detecting pinhole to remove out-of-focus light. This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 50um), DIC, a removable heating stage with CO2 delivery, and photomanipulation modules (Phasor and Ablate!). The scanhead allows 1x or 2x magnification (for extra magnification and to allow Nyquist sampling and deconvolution).

Data files older than 1 month will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.

System components

LASERs

LASER Wavelength Maximum Power
Violet 405 nm 100 mW
Blue-Violet 445 nm 150 mW
Blue 488 nm 150 mW
Yellow-Green 561 nm 100 mW
Orange 594 nm 100 mW
Red 637 nm 140 mW
Ablation 355 nm 60 μJ/200 Hz

Filtersets (eyepieces)

Position Filterset Excitation Dichroic Emission
2 (lower deck) Green 457-487 nm 495 nm 503-538 nm
3 (lower deck) Orange 542-582 nm 593 nm 604-644 nm
4 (lower deck) Blue 352-404 nm 409 nm 417-477 nm
3 (upper deck) Phasor / Ablation - < 380 nm -

Objectives

Magnification Model Immersion NA WD (mm) Reference
10x Plan Apochromat Air 0.45 4 MRD70170
20x Plan Fluor Oil, W, G, Sil 0.75 0.51–0.33 MRH07241
40x Plan Apochromat Air 0.95 0.21 MRD70470
40x Plan Apochromat Silicone Oil 1.25 0.3 MRD73400
60x Plan-Apochromat Water 1.20 0.31–0.28 MRD07602
100x Plan Apochromat Oil 1.45 0.13 MRD71970

Upon request:

Magnification Model Immersion NA WD (mm) Reference
40x Apochromat Water 1.25 0.2–0.16 MRD77400

Emission Filters CSU W1

Position Filter name Dichroic Transmission Reference
1 QUAD (c###q) 405/488/561/640 420-460 nm Semrock FF01-440/521/607/700
511-532 nm
590-624 nm
678-723 nm
2 c405s 405/488/561/640 423-468 nm Semrock FF01-445/45
3 c488s 405/488/561/640 510-540 nm Semrock FF01-525/30
4 c561s 405/488/561/640 581-654 nm Semrock FF02-617/73
5 c594s 445/515/594 602-682 nm Chroma ET642/80
6 c637s 405/488/561/640 672-712 nm Semrock FF01-692/40
7 DUAL (c###d) 405/488/561/640 510-540 nm Semrock EM01-R488/568
602-682 nm
8 c445s 445/515/594 470-495 nm Semrock FF01-482/25
9 c488/YFP 405/488/561/640 529-556 nm Semrock FF01-542/27
10 cBF-DIC - any -

Camera

Model Frame Size Pixel Size (µm) Quantum Efficiency
Photometrics Prime 95B 1200 x 1200 11 x 11 > 95%

Microscope Turn On Procedure

Microscope Turn Off Procedure

If there is another user for this microscope during the day:

Else:

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