Table of Contents

Location: [Oeiras] Room 0B05
Manufacturer: Nikon
Model: Nikon Eclipse Ti
Nickname: Nikon HTM-HCS
Software: NIS-Elements
Year: 2015
SN:
 
Data will be deleted after: 1 month  
 
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Microscope overview

The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols.

The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an ORCA-Flash4.0 LT+ sCMOS camera with 4.0 MPixels (2048×2048) with a large field of view, low-light sensitivity, and large dynamic range.

The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only).

This microscope is also equipped with a Ionoptix Calcium and Contractility system for high-speed simultaneous acquisition of Fura-2 fluorescence with video-based cell length measurement to study myocyte function, contracting spontaneously or with field stimulation.

System Components

LEDs

This system is equipped with a CoolLED fluorescence illuminator with 4 excitation lines:

Line Wavelength
UV 380 nm
B 470 nm
G 550 nm
R 635 nm

Filtersets Turret 1 (top)

Position Filterset Excitation Dichroic Emission Reference
1 QUAD 349-407 nm 409 nm 412-452 nm FL-007110
459-489 nm 493 nm 497-533 nm
541-567 nm 573 nm 577-613 nm
624-646 nm 652 nm > 658 nm
2 Green 460-488 nm 495 nm 503-548 nm
3 Orange 532-554 nm 562 nm 573-613 nm
4 Red 624-642 nm 652 nm 659-701 nm

Filtersets Turret 2 (bottom)

Position Filterset Excitation Dichroic Emission
6 Fura-2 334-346 nm 400 nm 498-518 nm
370-390 nm

Emission Filters Fast Filter Wheel

Filterset Emission Reference
DAPI-FW 435-485 nm AT460/50m
FITC-FW 500-550 nm ET525/50m
TRITC-FW 570-620 nm ET595/50m
Cy5-FW 665-705 nm S685/40m

Objectives

Magnification Model Immersion NA WD (mm) PFS DIC PhC Reference Pixel size (um) (1x zoom) Pixel size (um) (1.5x zoom)
10x Plan Apo Air 0.45 4 No Yes (N1) No MRD70170 0.643 0.429
20x Plan Apo Lambda Air 0.75 1 Yes Yes (N1) No MRD00205* 0.323 0.216
40x Plan Fluor Oil 1.3 0.24 Yes Yes (N2) No MRH01401 0.162 0.108
40x Plan Apo Air 0.95 0.17-0.25 Yes Yes (N2) No MRD00400* 0.161 0.107
40x ELWD* * Air 0.60 2.80-3.60 Yes No Yes MRH48430 0.161 0.108
100x Plan Apo Lambda Oil 1.45 0.13 Yes Yes (N2) No MRD01905* 0.065 0.043

* Not fabricated anymore ; * * LWD - Long Working Distance

The Bioimaging Platform highly advises against using the 40x LWD objective with 1.5x zoom

Camera

Model Frame Size Pixel Size (µm) Quantum Efficiency
ORCA-Flash4.0 LT PLUS 2048 × 2048 6.5 x 6.5 82%

Microscope Turn On Procedure

Microscope Turn Off Procedure

If there is another user for this microscope in the next hour:

Else:

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