Location: iMM Fish Facility
Manufacturer: Carl Zeiss MicroImaging
Model: SteREO Lumar V.12Axio Zoom.V16
Nickname: "Lumar"
Software: AxioVision
Year: 2009
SN: 3918000768
 
 
 
 
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===== Microscope overview ===== The Zeiss Lumar V12 is a //fluorescence stereo microscope// suitable for high resolution 3D analysis of large specimens in fluorescent and transmitted light. The system is equipped with fluorescence filters for GFP, Cy3 and Cy5.5 and a sensitive cooled CCD camera (Hamamatsu ORCA-R2) that allows you to capture weak fluorescent signals and minimize photobleaching in light sensitive samples. With the Zeiss Lumar V12 you can perform multi-channel, Z-stack and time-lapse image acquisition of your samples, using two different objectives with different working distances and magnification values. If you need higher magnifications, check out the [[leica_dm5000b|Leica DM5000B]], the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] or the [[zeiss_cell_observer|Zeiss Cell Observer]] widefield microscopes. {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. ===== System components ===== ==== Filtersets ==== ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | 1 | Empty ||||| | 2 | GFP | [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=488013-0000-000&o=|FS13]] | 460-480 nm | 495 nm | 505-530 nm | | 3 | Cy3 | [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | 532-557 nm | 570 nm | 570-640 nm | | 4 | Cy5.5 | [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=000000-1031-354&o=|FS32]] | 652-677nm | 695 nm | 700-750 nm | ==== Objectives ==== ^ Magnification ^ Model ^ WD (mm) ^ About ^ | 0.8x | NeoLumar S | 80 | for visual magnification from 6.4x to 80x with eyepieces 10x | **Upon Request:** ^ Magnification ^ Model ^ WD (mm) ^ About ^ | 1.5x | NeoLumar S | 30 | for visual magnification from 12x to 150x with eyepieces 10x | ==== Camera ==== ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | Hamamatsu ORCA-R2 | CCD | 1344 X 1024 | 6.45 x 6.45 | ===== Software Modules ===== * Multichannel Acquisition * Z-stack Acquisition * Extended Focus ---- [[resources|Back to the Equipment page]]