Location: [Oeiras] Room 0B06
Manufacturer: ZEISS Microscopy
Model: LSM 980 with Airyscan 2
Nickname: "980"
Software: ZEN 3.12
Year: 2019
SN: 2871000107
 
Data will be deleted after: 1 month  
 
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→ ZEISS LSM 980 with Airyscan 2 Usage Statistics

===== Microscope overview ===== {{ :confocal_pinhole.png?340}} The Zeiss LSM 980 with Airyscan 2 is an inverted [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] equipped with a laser scanning head with a total of 6 PMT/GaAsp + 1 Airyscan detectors. The Airyscan can achieve non-diffraction limited optical sectioning with resolutions down to 140nm laterally and ~ 400nm axially. It also allows acquisition in “Multiplexing” modes, which accelerate the acquisition speed (4/8x faster) or in sensitivity mode that enhances the signal-to-noise acquisition. The microscope is equipped with a dark large cage incubator and atmospheric control chamber to allow live imaging of mammalian cells (O2 control is also possible, upon request). We have available an objective inverter for some intra-vital or open-dish applications (upon request only, requires preparation). * **Microscope**: [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research.html|Zeiss Axio Observer]] * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 7]] * **Features** * | [[https://www.imdik.pan.pl/images/Us%C5%82ugi_dokumenty/Przydatne_informacje/2d_Definite_Focus_information.pdf|Definite Focus.3]] * | [[https://www.youtube.com/watch?v=tUiEa7vcggQ|Airyscan 2 Multiplex]] * | [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|LSM Plus]] * | [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|Joint Deconvolution]]

* [[https://www.youtube.com/watch?v=zA4xqq-EL9g|ZEISS Webinar: LSM 980 Airyscan 2 Multiplex mode]] {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. ===== System components ===== ==== LASERs ===== ^ LASER ^ Wavelength ^ Maximum Power ^ | Violet | 405 nm | 15 mW | | Blue | 488 nm | 13 mW | | Yellow-green | 561 nm | 13 mW | | Red | 639 nm | 10 mW | If you require more laser lines (e.g., 445 nm, 514 nm, or 594 nm), we suggest as an alternative the [[https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_lsm_980-32|[Lisbon] ZEISS LSM 980-32 with Airyscan 2]]. ==== Filtersets (for eyepieces) ==== ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | Blue | G 365 nm | 395 nm | 420-470 nm | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | | Green | 450-490 nm | 493 nm | 500-550 nm | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | | Red | 533-558 nm | 570 nm | 570-640 nm | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | ==== Objectives ==== ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | 2.5x | | - | 0.085 | 8.8 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420320-9902-000/Objective-EC-Plan-Neofluar-2.5x-0.085-M27|420320-9902-000]] | | 10x | EC Plan-Neofluar | Air | 0.45 | 2.0 | [[https://www.micro-shop.zeiss.com/en/ee/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | | 20x | Plan-Apochromat | Air | 0.75 | 0.55 | [[https://www.micro-shop.zeiss.com/en/ee/shop/objectives/420150-9900-000/Objective-Fluar-20x-0.75-M27|420150-9900-000]] | | 25x | Plan-Apochromat | W/Sil/Gly/Oil * | 0.80 | 0.57 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420852-9872-000/Objective-LD-LCI-Plan-Apochromat-25x-0.8-Imm-Corr-DIC-M27|420852-9872-000]] | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.14 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421782-9900-799/Objective-C-Plan-Apochromat-63x-1.4-Oil-DIC-M27|421782-9900-799]] | * please ask staff for help setting up the proper immersion medium setting for this 25x objetive, which can be used with cleared samples, and image successfully down to 450um (for example, using RAPIclear). ==== Airyscan 2 emission filters ===== ^ Position ^ Emission ^ | 1 | Plate | | 2 | BP 420 - 480 + BP 495 - 550 | | 3 | BP 420 - 470 + BP 570 - 630 | | 4 | BP 420 - 500 + LP 605 | | 5 | BP 465 - 505 + BP 525 - 585 | | 6 | BP 495 - 550 + BP 570 - 630 | | 7 | BP 495 - 555 + LP 660 | | 8 | BP 570 - 620 + LP 660 | ==== Detectors ==== * 1x blue-green multialkali PMT (15-20% QE) * 4x GaAsP PMT spectral detectors (tunable between 450-750 nm) (~45% QE) * 1x green-red multialkali PMT (10-25% QE) * 1x T-PMT detector (brighfield) * 1x Airyscan2 detector (~45% QE after processing Airyscan data) ===== How to start your session ===== If you are the **first user** of the day: * Turn off Main Switch {{::O_LSM980_MS.png?100|}} * Turn on the computer * Turn on HXP, and incubation module (if needed) {{::Oeiras_LSM980_hxp.png?129|}} {{::Oeiras_LSM980_incubation.png?100|}} * Turn on Main Switch * Log in to Windows with your Agendo credentials * Start **ZEN** Else: * Log in to Windows with your Agendo credentials * Start **ZEN** ===== How to end your session ===== If there is another user: * Clean up immersion objectives * Exit the ZEN software * Log off the computer * Turn off HXP, and incubation module (if > 1 hour for the next user) If you are the **last user** of the day: * Clean up immersion objectives * Exit the ZEN software * Turn off the computer * Turn off HXP, and incubation module (if used) ---- [[resources|Back to the Equipment page]]