====== Zeiss LSM 510 (no longer available) ======
{{510_whole.jpg?0x220|}} {{510_stand.jpg?0x220|}}
**Location**: no longer available
**Manufacturer**: Zeiss
**Model**: LSM 510
**Year**: 1998
**SN**: 450240
===== Microscope overview =====
The LSM 510 system is a point scanning laser confocal microscope prepared for live imaging and photobleaching experiments. The system can be equipped with a small size incubator for temperature control.
==== LASER units ====
^ Laser Unit ^ Wavelength ^ Maximum Power ^ Current Status ^
| Argon | 458, 488 and 514 nm | 25 mW | **not working** |
| HeNe1 | 543 nm | 1 mW | **not working** |
| HeNe2 | 633 nm | 5 mW | ok |
==== Objectives ====
^ Magnification ^ Class ^ Immersion ^ NA ^ More info ^
| 10x | Plan-Neofluar | No | 0.30 | [[https://www.micro-shop.zeiss.com/us/us_en/objektive.php?cp_sid=&f=od&p[]=440330-0000-000|440330-0000]] |
| 63x | Plan-Apochromat | Oil | 1.40 | [[https://www.micro-shop.zeiss.com/us/us_en/objektive.php?cp_sid=&f=od&p[]=440761-9905-000|440761-9905]] |
| 100x | Plan-Neofluar | Oil | 1.30 | [[https://www.micro-shop.zeiss.com/us/us_en/objektive.php?cp_sid=&f=od&p[]=440480-9903-000|440480-9903]] |
==== Filter and Dichroic sets ====
| {{510_main.jpg?120|}} | {{510_emmission1.jpg?120|}} | {{510_emmission2.jpg?120|}} | {{510_2dichroic.jpg?120|}} | {{510_1dichroic.jpg?120|}} |
| Main configuration window | Emission filters for channel 1 | Emission filters for channel 2 | Channel separation dichroic | Laser separation dichroic |
===== Microscope Turn On Procedures =====
* Turn on the two UPS near the microscope.
{{zeiss_lsm510_ups.jpg?100}}
* Turn the remote control on.
{{zeiss_lsm510_remote_control.jpg?100}}
* Turn the HBO lamp on.
{{zeiss_lsm510_fluorescence.jpg?100}}
* Turn the halogen transmitted light power source on.
{{zeiss_lsm510_transmited.jpg?100}}
* Turn the computer on.
* Login to the computer, start the LSM software.
===== Microscope Turn Off procedures =====
If there's another user for this microscope in the next two hours:
* Close the software, leave the lasers on an log off the computer.
* Clean up any objective were you used immersion oil.
* Make sure that there really is another user going to use the microscope.
Else:
* Clean up any objective were you used immersion oil.
* Turn off the lasers in the software (wait for laser cooling!)
* Clean up immersion objectives
* Turn off the HBO lamp.
* Turn off the transmitted light lamp.
* Shutdown the computer.
* Turn off the remote control.
* Turn off the two ups.
===== External links =====
* This {{lsm510.pdf|PDF}} shows how to work with the system, it's not exactly the same system configuration but teaches a lot about the software.
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[[resources|Back to the Equipment page]]
Zeiss LSM 510 Booking
Zeiss LSM 510 Usage Report