Location: [Oeiras] Room 0B03
Manufacturer: ZEISS
Model: Stereo Lumar V.12
Nickname: Lumar
Software: Micromanager (Hamamatsu camera) / uEye (uEye camera)
Year: 2011
SN:

Data will be deleted after: 3 months
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===== Microscope overview ===== The Stereo Lumar V.12 is a motorized stereoscope with fluorescence, capable of imaging in colour and grayscale (camera dependent), with a zoom between 9.6x and 120x. ===== System components ===== ==== Filtersets ==== ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | 1 | Cyan | 426-446 nm | 455 nm | 460-500 nm | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488047-9100-400|FS47]] | | 2 | Green | 460-490 nm | 495 nm | 505-530 nm | FS13 | | 3 | Red | 540-580 nm | 585 nm | 592-667 nm | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488045-9100-400|FS45]] | | 4 | Yellow | 490-510 nm | 515 nm | 520-550 nm | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/000000-1196-681|FS46]] | ==== Camera ==== Camera can be changed on request, and training for this can be provided if needed. ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ Installed date ^ | [[https://en.ids-imaging.com/store/ui-3370cp-rev-2.html|UI-3370CP Rev. 2]] Color Camera | 2048 × 2048 | 5.5 x 5.5 | - | Since Oct 2024 | | [[https://web.archive.org/web/20241014073627/https://hep.hamamatsu.com/eu/en/products/C11440-42U30.html|ORCA-Flash4.0 LT+]] | 2048 × 2048 | 6.5 x 6.5 | 82% | Before May 5, 2025 | | ORCA-Flash4.0 - C11440-22C | 2048 × 2048 | 6.5 x 6.5 | 82% | After May 5, 2025 | ==== Pixel-size vs magnification ==== ^ Magnification ^ Hamamatsu (µm/pixel) ^ IDS (µm/pixel) ^ | 9.6x | 10.90 | 9.40 | | 10.0x | 10.49 | 9.02 | | 20.0x | 5.07 | 4.52 | | 30.0x | 3.41 | 3.02 | | 40.0x | 2.59 | 2.26 | | 50.0x | 2.05 | 1.81 | | 60.0x | 1.73 | 1.51 | | 70.0x | 1.49 | 1.30 | | 80.0x | 1.29 | 1.13 | | 90.0x | 1.16 | 1.01 | | 100.0x | 1.04 | 0.91 | | 110.0x | 0.95 | 0.83 | | 120.0x | 0.87 | 0.76 | ===== Microscope Turn On Procedure ===== * Turn on the **powerbar below the stone table**. * **If fluorescence is necessary**, turn on the **fluorescent lamp controller** on top of the desk. ===== Microscope Turn Off Procedure ===== * Turn on the **powerbar below the stone table**. * **If fluorescence was used**, turn off the **fluorescent lamp controller** on top of the desk. * This lamp **has to be on for 30 minutes**. If you use the system less than 30 minutes, **wait** until that time to turn the lamp off, or **warn the Bioimaging Team**. ---- [[resources|Back to the Equipment page]]