Location: [Oeiras] Room 0B05
Manufacturer: Nikon
Model: Nikon Eclipse Ti
Nickname: Nikon HTM-HCS
Software: NIS-Elements
Year: 2015
SN:
 
Data will be deleted after: 1 month  
 
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===== Microscope overview ===== The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols. The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an Andor Zyla 4.2 sCMOS camera with 4.0 MPixels with a large field of view capable of maximum of 100 FPS at full frame (2048x2048). The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only). {{::ionoptix_realtime-acquisition-cell-length-600x500.jpg?200 |}} This microscope is also equipped with a [[https://www.ionoptix.com/products/systems/calcium-and-contractility-system/|Ionoptix Calcium and Contractility system]] for high-speed simultaneous acquisition of Fura-2 fluorescence with video-based cell length measurement to study myocyte function, contracting spontaneously or with field stimulation. * **High-speed contractility camera**: [[https://www.ionoptix.com/products/components/sensors/myocam-s3/|MyoCam-S3]] * **Electrical Field Stimulator**: [[https://www.ionoptix.com/products/components/stimulators/myopacer-cell-stimulator/|MyoPacer]] * **LED Light Source**: [[https://www.ionoptix.com/products/components/light-sources/led-light-source/|UV LEDs for Fura-2 excitation]] * **High-speed PMT**: [[https://www.ionoptix.com/products/components/sensors/pmt-sub-system/|PMT400]] for photon counting at 1kHz * **Software**: [[https://www.ionoptix.com/products/software/ionwizard-core-and-analysis/|IonWizard]] 7.7.3.180 ===== System Components ===== ==== LEDs ==== This system is equipped with a CoolLED fluorescence illuminator with 4 excitation lines: ^ Line ^ Wavelength ^ | UV | 380 nm | | B | 470 nm | | G | 550 nm | | R | 635 nm | ==== Filtersets Turret 1 (top) ==== ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | 1 | QUAD | 349-407 nm | 409 nm | 412-452 nm | [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|FL-007110]] | | ::: | ::: | 459-489 nm | 493 nm | 497-533 nm | ::: | | ::: | ::: | 541-567 nm | 573 nm | 577-613 nm | ::: | | ::: | ::: | 624-646 nm | 652 nm | > 658 nm | ::: | | 2 | Green | 460-488 nm | 495 nm | 503-548 nm | | | 3 | Orange | 532-554 nm | 562 nm | 573-613 nm | | | 4 | Red | 624-642 nm | 652 nm | 659-701 nm | | ==== Filtersets Turret 2 (bottom) ==== ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ | 6 | Fura-2 | 334-346 nm | 400 nm | 498-518 nm | | ::: | ::: | 370-390 nm | ::: | ::: | ==== Emission Filters Fast Filter Wheel ==== ^ Filterset ^ Emission ^ Reference ^ | DAPI-FW | 435-485 nm | [[https://www.chroma.com/products/parts/at460-50m|AT460/50m]] | | FITC-FW | 500-550 nm | [[https://www.chroma.com/products/parts/et525-50m|ET525/50m]] | | TRITC-FW | 570-620 nm | [[https://www.chroma.com/products/parts/et595-50m|ET595/50m]] | | Cy5-FW | 665-705 nm | [[https://www.chroma.com/products/parts/s685-40m|S685/40m]] | ==== Objectives ==== ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ PFS ^ DIC ^ PhC ^ Reference ^ Pixel size (um) (1x zoom) ^ Pixel size (um) (1.5x zoom) ^ | 10x | Plan Apo | Air | 0.45 | 4 | No | Yes (N1) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-179798|MRD70170]] | 0.643 | 0.429 | | 20x | Plan Apo Lambda | Air | 0.75 | 1 | Yes | Yes (N1) | No | MRD00205* | 0.323 | 0.216 | | 40x | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]] | 0.162 | 0.108 | | 40x | Plan Apo | Air | 0.95 | 0.17-0.25 | Yes | Yes (N2) | No | MRD00400* | 0.161 | 0.107 | | 40x | LWD* * | Air | 0.55 | 2.1 | No | No | Yes | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1683|MRP46402]] | 0.161 | 0.108 | | 100x | Plan Apo Lambda | Oil | 1.45 | 0.13 | Yes | Yes (N2) | No | MRD01905* | 0.065 | 0.043 | * Not fabricated anymore ; * * LWD - Long Working Distance The Bioimaging Platform **highly advises against using the 40x LWD objective with 1.5x zoom** ==== Camera ==== ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | [[https://andor.oxinst.cn/assets/uploads/products/andor/documents/Zyla-4.2-Specifications-Life-Science.pdf|Andor Zyla 4.2 PLUS]] | 2048 × 2048 | 6.5 x 6.5 | 82% | ===== Microscope Turn On Procedure ===== * Turn on the microscope using the **switch** on the **bottom of the microscope's optical table**. {{nikon-htm_turnon_3.png?200|}} ===== Microscope Turn Off Procedure ===== * Turn off the microscope using the **switch** on the **bottom of the microscope's optical table**. {{nikon-htm_turnon_3.png?200|}} ---- [[resources|Back to the Equipment page]]