Location: [Oeiras] Room 0B02
Manufacturer: Nikon
Model: Nikon Eclipse Ti
Nickname: Nikon HTM-HCS
Software: NIS-Elements
Year: 2015
SN:
 
Data will be deleted after: 1 month  
 
→ Nikon HTM-HCS Quality Control
→ Nikon HTM-HCS Usage Statistics

===== Microscope overview ===== The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols. The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an Andor Zyla 4.2 sCMOS camera with 4.0 MPixels with a large field of view capable of maximum of 100 FPS at full frame (2048x2048). The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only). ===== LED lines ===== This system is equipped with a CoolLED fluorescence illuminator with 4 excitation lines: * 380 nm - E.g. DAPI * 470 nm - E.g. FITC * 550 nm - E.g. TRITC * 635 nm - E.g. Cy5 ===== Filterset cubes ===== ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | 1 | Quad-DAPI | | 378/58 | [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|409]] | 432/40 | | 1 | Quad-FITC | | 474/30 | [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|493]] | 515/36 | | 1 | Quad-TRITC | | 554/26 | [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|573]] | 595/36 | | 1 | Quad-Cy5 | | 635/22 | [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|652]] | LP 658 | | 2 | FITC | | 474/27 | 495 | 525/45 | | 3 | TRITC | | 543/22 | 562 | 593/40 | | 4 | Cy5 | | 635/18 | 652 | 680/42 | ===== Fast Filter Wheel emission filters ===== ^ Filterset ^ Reference ^ Transmission ^ | DAPI-FW | [[https://www.chroma.com/products/parts/at460-50m|at460-50m]] | 460/50 | | FITC-FW | [[https://www.chroma.com/products/parts/et525-50m|et525-50m]] | 525/50 | | TRITC-FW | [[https://www.chroma.com/products/parts/et595-50m|et595-50m]] | 595/50 | | Cy5-FW | [[https://www.chroma.com/products/parts/s685-40m|s685-40m]] | 685/40 | ===== Objectives ===== ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ PFS ^ DIC ^ PhC ^ Reference ^ Pixel size (um) (1x zoom) ^ Pixel size (um) (1.5x zoom) ^ | 10x | Plan Apo | Air | 0.45 | 4 | No | Yes (N1) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-179798|MRD70170]] | 0.643 | 0.429 | | 20x | Plan Apo Lambda | Air | 0.75 | 1 | Yes | Yes (N1) | No | MRD00205* | 0.323 | 0.216 | | 100x | Plan Apo Lambda | Oil | 1.45 | 0.13 | Yes | Yes (N2) | No | MRD01905* | 0.065 | 0.043 | | 40x | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]] | 0.162 | 0.108 | | 40x | Plan Apo | Air | 0.95 | 0.17-0.25 | Yes | Yes (N2) | No | MRD00400* | 0.161 | 0.107 | | 40x | LWD* * | Air | 0.55 | 2.1 | No | No | Yes | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1683|MRP46402]] | 0.161 | 0.108 | * Not fabricated anymore ; * * LWD - Long Working Distance The Bioimaging Platform **highly advises against using the 40x LWD objective with 1.5x zoom** ===== Camera ===== ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | [[https://andor.oxinst.cn/assets/uploads/products/andor/documents/Zyla-4.2-Specifications-Life-Science.pdf|Andor Zyla 4.2 PLUS]] | 2048 × 2048 | 6.5 x 6.5 | 82% | ===== Microscope Turn On Procedure ===== * Turn on the CoolLed controller on **top of the microscope's shelf**. {{nikon-htm_turnon_1.png?200|}} * Turn on the microscope using the **switch** on the **bottom of the microscope's optical table**. {{nikon-htm_turnon_2.png?200|}} ===== Microscope Turn Off Procedure ===== * Turn off the microscope using the **switch** on the **bottom of the microscope's optical table**. {{nikon-htm_turnon_2.png?200|}} * Turn off the CoolLed controller on **top of the microscope's shelf**. {{nikon-htm_turnon_1.png?200|}} ---- [[resources|Back to the Equipment page]]