Location: Room P2-A-24 ( 47219)
Manufacturer: Nikon Instruments
Model: Eclipse Ti
Nickname: "006"
Software: NIS-Elements AR 5.42.05
Year: 2010
SN: 602805
 
Data will be deleted after: 3 months  
 
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===== Microscope overview ===== {{ :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply, and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with an Andor Neo 5.5 monochrome cooled sCMOS camera. * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] * **Camera**: [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]] This microscope is also equipped with a [[https://www.ionoptix.com/products/systems/calcium-and-contractility-system/|Ionoptix Calcium and Contractility system]] for high-speed simultaneous acquisition of Fura-2 fluorescence with video-based cell length measurement to study myocyte function, contracting spontaneously or with field stimulation. {{::ionoptix_realtime-acquisition-cell-length-600x500.jpg?200 |}} * **High-speed contractility camera**: [[https://www.ionoptix.com/products/components/sensors/myocam-s3/|MyoCam-S3]] * **Electrical Field Stimulator**: [[https://www.ionoptix.com/products/components/stimulators/myopacer-cell-stimulator/|MyoPacer]] * **LED Light Source**: [[https://www.ionoptix.com/products/components/light-sources/led-light-source/|UV LEDs for Fura-2 excitation]] * **High-speed PMT**: [[https://www.ionoptix.com/products/components/sensors/pmt-sub-system/|PMT400]] for photon counting at 1kHz * **Software**: [[https://www.ionoptix.com/products/software/ionwizard-core-and-analysis/|IonWizard]] 7.7.3.180 {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. ===== System components ===== ==== Filtersets Turret 1 (top) ==== ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | 1 | Empty ||||| | 2 | DAPI | MBE41300 | 340-380 nm | 400 nm | 435-485 nm | | 3 | FITC | MBE44720 | 465-495 nm | 505 nm | 515-555 nm | | 4 | TRITC | MBE45600 | 528-553 nm | 565 nm | 590-650 nm | | 5 | Cy5 | 2SK4CY50 | 608-648 nm | 660 nm | 672-712 nm | | 6 | Empty ||||| ==== Filtersets Turret 2 (bottom) ==== ^ Position ^ Filterset ^ | 1 | Empty | | 2 | Empty | | 3 | Empty | | 4 | Empty | | 5 | Empty | | 6 | Fura-2 | ==== Objectives ==== ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | | 20x | CFI S Plan Fluor ELWD ADM Ph1 | Air | 0.45 | 6.90 - 8.20 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1813|MRH48230]] | | 40x | CFI CFI Plan Apochromat Lambda D | Air | 0.95 | 0.21 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179806|MRH70470]] | | 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]] | | 60x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | | | 100x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | | **Upon request:** ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | 100x | CFI Plan | Water | 1.10 | 2.50 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1956|MRL07920]] | ==== Camera ==== ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]] | CCD | 2560 x 2160 | 6.5 x 6.5 | ---- [[resources|Back to the Equipment page]]