Location: Lab 19 (floor 0)
Manufacturer: Nikon Instruments
Model: Eclipse TE2000-E
Nickname: "Asterix"
Software: Metamorph / NIS-Elements
Year: 2007
SN: 596701

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→ Nikon Eclipse TE2000-E Usage Statistics

===== Microscope overview ===== {{ :widefield_lightpath.png?0x300}}The Nikon Eclipse TE2000-E is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a CoolSNAP EZ cooled monochrome CCD camera for fast, high-resolution imaging. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[big_guy|Big Guy]] or [[colossus|Colossus]]. * **Microscope**: [[https://www.microscopyu.com/museum/eclipse-te2000-inverted-microscope|Nikon Eclipse TE2000-E]] * **Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/CoolSNAP-EZ-Datasheet.pdf|Photometrics CoolSNAP EZ mono CCD]] {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. ===== System components ===== ==== Filtersets ==== ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | 1 | DAPI | MBE41300 | 340-380 nm | 400 nm | 435-485 nm | | 2 | TRITC | MBE45600 | 528-553 nm | 565 nm | 577-632 nm | | 3 | AF | none |||| | 4 | Empty ||||| | 5 | GFP | MBE44720 | 465-495 nm | 505 nm | 515-555 nm | | 6 | Cy5 | 2SK4CY50 | 608-648 nm | 660 nm | 672-712 nm | ==== Objectives ==== ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | | 40x | CFI Plan Fluor DLL Ph2 | Air | 0.75 | 0.66 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1838|MRH10401]] | | 60x | CFI S Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1876|MRD31605]] | **Upon request:** ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | 40x | CFI S Plan Fluor ELWD ADL Ph 2 | Air | 0.60 | 2.70 - 3.70 | | | 100x | CFI Plan | Water | 1.10 | 2.50 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1956|MRL07920]] | ==== Camera ==== ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | [[https://www.photometrics.com/wp-content/uploads/2019/10/CoolSNAP-EZ-Datasheet.pdf|CoolSNAP EZ mono CCD]] | CCD | 1392 x 1040 | 6.45 x 6.45 | ---- [[resources|Back to the Equipment page]]