Location: [Oeiras] Room 0B02
Manufacturer: Leica Microsystems
Model: Leica STELLARIS STED
Nickname: "STELLARIS"
Software: LAS X
Year: 2026
SN: 840000544
 
Data will be deleted after: 1 month  
 
→ Leica STELLARIS STED FLIM Quality Control
→ Leica STELLARIS STED FLIM Usage Statistics

===== Microscope overview ===== {{ :sted_principle.png?240}} The Leica STELLARIS STED FLIM is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] designed for advanced fluorescence imaging, [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-fluorescence-lifetime-imaging-microscopy-flim/|fluorescence lifetime imaging (FLIM)]] and super-resolution microscopy. Built on the [[https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-stellaris/|Leica STELLARIS platform]], it features a [[https://www.leica-microsystems.com/science-lab/life-science/stellaris-white-light-lasers/|White Light LASER]] (440-790 nm) and 405 nm laser as excitation sources, AOBS beam splitting, highly sensitive [[https://www.leica-microsystems.com/science-lab/life-science/power-hyd-detectors/|Power HyD detectors]] and spectral detection for flexible multicolor imaging, including near-infrared fluorophores up to 850 nm, such as Cy7. The system is optimized for live-cell imaging, featuring a motorized stage, SuperZ Galvo Stage for rapid Z acquisition, hardware autofocus (AFC) and a temperature- and CO₂-controlled Okolab dark enclosure for live-cell imaging in physiological conditions. FLIM capabilities are provided by the Power HyD X detector and [[https://www.leica-microsystems.com/products/confocal-microscopes/p/stellaris-8-falcon/|STELLARIS FALCON (FAst Lifetime CONtrast)]], enabling fast lifetime imaging, [[https://www.leica-microsystems.com/science-lab/life-science/what-is-fret-with-flim/|FLIM-FRET experiments]], [[https://www.leica-microsystems.com/science-lab/life-science/phasor-analysis-for-flim-fluorescence-lifetime-imaging-microscopy/|phasor analysis]] and [[https://www.leica-microsystems.com/science-lab/life-science/tausense-technology-imaging-tools/|TauSense]] lifetime-based contrast and multiplexing tools. For super-resolution imaging, the system is equipped with [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-super-resolution/|2D and 3D STED technology]] using pulsed 589 nm and 775 nm depletion lasers, providing resolution well below the diffraction limit. [[https://www.leica-microsystems.com/science-lab/life-science/extended-live-cell-imaging-at-nanoscale-resolution/|TauSTED Xtend]] combines STED and lifetime information to improve image quality and resolution while reducing light exposure, making the system particularly suitable for live-cell nanoscale imaging. * **Microscope**: [[https://www.leica-microsystems.com/products/light-microscopes/p/dmi8/|Leica DMi8 CS inverted]] * **LED Illumination**: [[https://www.coolled.com/products/pe-300white/|CoolLED pE-300white]] * **Detectors**: [[https://www.leica-microsystems.com/science-lab/life-science/power-hyd-detectors/|Power hybrid detectors]] * Power HyD S * Power HyD X for FLIM imaging * Power HyD R for near-infrared imaging * **Features** * | [[https://www.leica-microsystems.com/science-lab/life-science/a-guide-to-super-resolution/|2D and 3D Super Resolution STED]] * | [[https://www.leica-microsystems.com/science-lab/life-science/extended-live-cell-imaging-at-nanoscale-resolution/|TauSTED Xtend]] * | [[https://www.leica-microsystems.com/science-lab/life-science/tausense-technology-imaging-tools/|TauSense]] * | [[https://www.leica-microsystems.com/products/confocal-microscopes/p/stellaris-8-falcon/|FALCON FLIM Imaging]] * | [[https://www.leica-microsystems.com/science-lab/life-science/obtain-maximum-information-from-your-specimen-with-lightning/|LIGHTNING adaptive deconvolution]] * | [[https://youtu.be/3uq42XefHhY?si=EWUFLyqk2z3Q1HG3|Adaptive Focus Control (AFC)]]

{{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. ===== Additional information for sample preparation ===== * [[https://downloads.leica-microsystems.com/STELLARIS%208%20STED/Application%20Note/STELLARIS%20STED%20Sample%20Preparation%20Guide.pdf|The Guide to STED Sample Preparation (application report)]] ===== System components ===== ==== LASERs ==== ^ STED LASER ^ Wavelength ^ | STED 589 | 589 nm | | STED 775 | 775 nm | ^ LASER ^ Wavelength ^ | Violet | 405 nm | | WLL | 440 nm - 790 nm | ==== Filterset (for eyepieces) ==== ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | Blue | 375-435 nm | 455 nm | 450-490 nm | [[https://www.fpbase.org/spectra/?s=6094,6092,6093&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|LED 405 (15525338)]] | | Green | 450-490 nm | 495 nm | 500-550 nm | GFP (15525314) | | Orange-Red | 518-562 nm | 570 nm | >590 nm | RHOD LP (15525303) | ==== Objectives ==== ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | 10x | HC PL APO CS2 | Air | 0.40 | 2.56 | [[https://www.leica-microsystems.com/objectivefinder/detail/objective/506424/|11506424]] | | 20x | HC PL APO IMM CORR CS2 | Water | 0.75 | 0.66 | [[https://www.leica-microsystems.com/objectivefinder/detail/objective/506343/|11506343]] | | 40x | HC PL APO Oil CS2 | Oil | 1.30 | 0.17 | [[https://www.leica-microsystems.com/objectivefinder/detail/objective/506428/|11506428]] | | 63x | HC PL APO Oil CS2 | Oil | 1.40 | 0.14 | [[https://www.leica-microsystems.com/objectivefinder/detail/objective/506350/|11506350]] | | 100x | HC PL APO OIL STED WHITE | Oil | 1.40 | 0.13 | [[https://www.leica-microsystems.com/objectivefinder/detail/objective/506378/|11506378]] | ---- [[resources|Back to the Equipment page]]