Location: [Lisbon] Rodent Platform
Manufacturer: Revvity
Model: IVIS Lumina LT
Nickname: "Lumina LT"
Software: Living Image 4.8.3
Year: 2025
SN: IS2533N10260

Data will be deleted after: 3 months

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===== System overview ===== The IVIS Lumina LT //fluorescence/bioluminescence imaging system// is equipped with 2D bioluminescence, fluorescence, and radioisoptic (Cerenkov) imaging capabilities, providing high sensitivity, ease of use, and flexibility to image fluorescent and/or bioluminescent reporters both //in vivo// and //in vitro//. The system includes a highly sensitive CCD camera, a light-tight imaging chamber with complete computer automation, and the Living Image software package for image acquisition and analysis. Features: * 2D Bioluminescence * 2D Fluorescence * Radioisotopic Cerenkov Imaging * Imaging of up to 5 mice * Compute Pure Spectrum Spectral Unmixing * Extended NIR Range * Absolute Calibration to NIST® Standards {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. ===== System booking rules ===== {{:warning.png?25|}} You can book the system with **30 days** in advance. ==== System Specifications ==== * **Camera sensor**: Back-thinned, back-illuminated CCD * **CCD cooling temperature**: -90 °C * **Frame size**: 1024 x 1024 * **Pixel size**: 13 μm * **Quantum Efficiency**: >85% Efficiency 500-700 nm, >55% Efficiency 400-500 nm, >35% Efficiency 700-900 nm * **Minimum FOV**: 5 x 5 cm * **Lens**: f/.95 – f/16, 50 mm * **Sensitivity**: 100 photons/s/cm²/sr * **Read noise**: < 3 electrons for bin = 1, 2, 4; < 5 electrons for bin = 8, 16 * **Dark Current**: < 120 electrons/s/cm2; or 2 x 10-4 electrons/s/pixel (typical) * **Excitation fluorescence filters**: 10 * **Emmission fluorescence filters**: 4 ==== Excitation and Emission Filtersets ==== ^ Excitation (nm) ^ Emission (nm) ^ | 430 | 515 - 575 | | 465 | 575 - 650 | | 500 | 695 - 770 | | 535 | 810 - 875 | | 570 | | 605 | | 640 | | 675 | | 710 | | 745 | ---- [[resources|Back to the Equipment page]]