Bioimaging Wiki

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<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lumar_v12_map.jpg">Room P3-A-44</a><br> 
<b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br>
<b>Model</b>: SteREO Lumar V.12Axio Zoom.V16<br>
<b>Software</b>: AxioVision<br>
<b>Year</b>: 2009 <br>
<b>SN</b>: 3918000768<br>
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&rarr; <a href="https://imm.medicina.ulisboa.pt/intranet/booking/month.php?user=ZEISSLUMA&rid=58"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png">  Zeiss Lumar V12 Booking</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_lumar_v12_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Zeiss Lumar V12 Usage Statistics</a>
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===== Microscope overview =====
The Zeiss Lumar V12 is a //fluorescence stereo microscope// suitable for high resolution 3D analysis of large specimens in fluorescent and transmitted light. The system is equipped with fluorescence filters for GFP, Cy3 and Cy5.5 and a sensitive cooled CCD camera ([[http://www.hamamatsu.com/jp/en/community/life_science_camera/product/search/C10600-10B/index.html|ORCA R2]]) that allows you to capture weak fluorescent signals and minimize photobleaching in light sensitive samples. With the Zeiss Lumar V12 you can perform multi-channel, Z-stack and time-lapse image acquisition of your samples, using two different objectives with different working distances and magnification values. If you need higher magnifications, check out the [[leica_dm5000b|Leica DM5000B]] or the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]].

===== System components =====

==== Filtersets ====

^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Emission  ^
|  1  |  Empty  |  None  |  None  |  None  |
|  2  |  GFP  |  [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=488013-0000-000&o=|FS13]]  |  460-480 nm  |  505-530 nm  |
|  3  |  Cy3  |  [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]]  |  532-557 nm  |  570-640 nm  |
|  4  |  Cy5.5  |  [[https://www.micro-shop.zeiss.com/?s=1249886915f0649&l=en&p=us&f=f&a=v&b=f&id=000000-1031-354&o=|FS32]]  |  652-677nm  |  700-750 nm  |

==== Objectives ====
^  Magnification   ^  Model  ^  WD (mm)  ^  About  ^
|  0.8x  |  NeoLumar S  |  80  |  for visual magnification from 6.4x to 80x with eyepieces 10x  |

**Upon Request:**
^  Magnification   ^  Model  ^  WD (mm)  ^  About  ^
|  1.5x  |  [[https://www.micro-shop.zeiss.com/?s=124984472807e9c&l=en&p=us&f=a&i=10157_400000002300420000&o=&h=25&n=0&sd=435225-9901-000#435225-9901-000|NeoLumar S]]  |  30  |  for visual magnification from 12x to 150x with eyepieces 10x  |


===== Software Modules =====

  * Multichannel Acquisition
  * Z-stack Acquisition
  * Extended Focus

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