Bioimaging Wiki

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<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiozoom_v16_map_new.png">[Lisbon] Room P0-A-30</a><br> 
<b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br>
<b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/stereo-and-zoom-microscopes/axio-zoom-v16-for-biology.html" target="_blank"> Axio Zoom.V16</a><br>
<b>Nickname</b>: "Axio Zoom"<br>
<b>Software</b>: ZEN 2012 (blue edition)<br>
<b>Year</b>: 2014 <br>
<b>SN</b>: 3941000138<br>
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Data will be deleted after: <b>3 months</b>
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&rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_axiozoom_v16_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Axio Zoom.V16 Quality Control</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_axiozoom_v16_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Zeiss Axio Zoom.V16 Usage Statistics</a>
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===== Microscope overview =====
The Zeiss Axio Zoom.V16 is a //fluorescence stereo microscope// suitable for imaging large samples such as complete organisms. The system is equipped with fluorescence filters for GFP and RFP and a monochromatic camera (AxioCam MRm). With the Zeiss Axio Zoom.V16 you can perform multi-channel, Z-stack and time-lapse image acquisition of your samples, using a 1x objective with a high numerical aperture of NA 0.25.

{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.

===== System components =====

==== Filtersets ====

^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Dichroic  ^  Emission  ^
|  1  |  GFP  |  [[https://www.micro-shop.zeiss.com/?s=163079566b5a5f8&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]]  |  450-490 nm  |  495 nm  |  500-550 nm  |
|  2  |  RFP  |  [[https://www.micro-shop.zeiss.com/?s=163079566b5a5f8&l=en&p=us&f=f&a=v&b=f&id=489063-0000-000&o=|FS63HE]]  |  559-585 nm  |  590 nm  |  600-690 nm  |
|  3  |  Empty  |||||
|  4  |  Cy5  |  [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/488050-9901-000|FS50]]  |  625-655 nm  |  660 nm  |  665-715 nm  |
==== Objectives ====
^  Magnification   ^  Model  ^  NA  ^  WD (mm)  ^  About  ^
|  1x  |  PlanNeoFluar Z  |  0.25  |  56  |  for visual magnification from 7x to 112x with eyepieces 10x  |

==== Camera ====
^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
|  [[https://qb3.berkeley.edu/wp-content/uploads/2020/07/Zeiss-AxioCam-MRm-Info_48-0065_e.pdf|AxioCam MRm]]  |  CCD  |  1388 X 1040  |  6.45 x 6.45  |

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