Bioimaging Wiki

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<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axioscan_7_map_2024.png">[Lisbon] Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> 
<b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br>
<b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/imaging-systems/axioscan-for-biology.html">Axioscan 7</a><br>
<b>Nickname</b>: "Axioscan"<br>
<b>Software</b>: ZEN 3.9 (ZEN Slidescan)<br>
<b>Year</b>: 2024 <br>
<b>SN</b>: 4661000477<br>
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Data will be deleted after: <b>3 months</b>
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&rarr; <a href="/facility/bioimaging/doku.php?id=zeiss_axioscan_7_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Zeiss Axioscan 7 Usage Statistics</a>
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===== Microscope overview =====
The Zeiss Axioscan 7 is a high-performance automated slide scanner for brightfield and fluorescence imaging. It can scan up to 100 slides in a single run. It is equipped with a fully motorized condenser, LED illumination and sensitive color and monochromatic cameras for high-speed digitization of high quality images. Using the ZEN software you can assign scan profiles for acquisition runs to be set up quickly whether you have many similar slides or mixed applications to process.  

  * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 7]]
  * **Camera** (for brightfield): [[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-705-color.html|Axiocam 705 color]]
  * **Camera** (for fluorescence): [[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-712-mono.html|Axiocam 712 mono]]

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<p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/u-tnLKIbxnk" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p>
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{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.

===== System components =====

==== LEDs  ====
^  Line  ^  Wavelength  ^
|  UV  |  385 nm  |
|  V  |  430 nm  |
|  B  |  475 nm  |
|  G  |  555 nm  |
|  Y  |  590 nm  |
|  R  |  630 nm  |
|  FR  |  735 nm  |

==== Filtersets ====

^  Filterset  ^  Reference  ^  Dichroic  ^  Emission  ^
|  LED SBP  |  [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489112-9010-000|112 SBP]]  |  405 nm  |  410-440 nm  |
|  :::  |  :::  |  493 nm  |  500-530 nm  |
|  :::  |  :::  |  575 nm  |  580-605 nm  |
|  :::  |  :::  |  654 nm  |  660-705 nm  |
|  :::  |  :::  |  761 nm  |  769-807 nm  |


Check here to check your experimental design in [[https://www.fpbase.org/spectra/?s=7754,222,223,204,205,2055,2057,262,263,2059,2068,306,307,$cf0_BP_425_30_90,$cf1_BP_515_30_90,$cf2_BP_593_25_90,$cf3_BP_683_45_90&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength | FPbase.org for a sample stained with DAPI+A488+A568+A647 (change at will!)]]


==== Objectives ====
^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  Reference  ^
|  5x  |  Fluar  |  Air  |  0.25  |  12.5  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420130-9900-000/Objective-Fluar-5x-0.25-M27|420130-9900-000]]  |
|  10x  |  Plan-Apochromat  |  Air  |  0.45  |  2.1  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]]  |
|  20x  |  Plan-Apochromat  |  Air  |  0.8  |  0.55  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]]  |
|  40x  |  Plan-Apochromat  |  Air  |  0.95  |  0.25  |  [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]]  |

==== Cameras ====
^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
|  [[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-705-color.html|Axiocam 705 color]]  |  CMOS  |  2464 x 2056  |  3.45 x 3.45  |
|  [[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-712-mono.html|Axiocam 712 mono]]  |  CMOS  |  4096 x 3008  |  3.45 x 3.45  |

===== System Turn On Procedures =====

  * Turn on the Axioscan 7
  * Turn on the computer
  * Login in to Windows with your Agendo credentials
  * Start the **ZEN** software

===== System Turn Off procedures =====
If there is another user for this microscope is the __next hour__:
  * Exit the ZEN software
  * Log off Windows
Else
  * Exit the ZEN software
  * Turn off the computer
  * Turn off the Axioscan 7

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