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====== Head of Facility ======

===== José Rino, PhD ===== 

{{jose_rino_2022.png?200  |}} \\ **Publishing Name**: José Rino

**Ciência ID**: A117-FBDB-A27B

**Nationality**: Portuguese

**E-mail address**: jose.rino@gimm.pt

\\

[[https://www.linkedin.com/in/jos%C3%A9-rino-9b02873b|{{linkedin.png?30}}]]
[[https://scholar.google.com/citations?hl=en&user=lcmhEqIAAAAJ|{{google_scholar_logo.png?30}}]]
[[https://orcid.org/0000-0002-0637-4527|{{orcid_id.png?30}}]]
[[https://www.researchgate.net/profile/Jose_Rino2|{{researchgate_icon.png?30}}]]

-----

==== Research History ====

José Rino graduated in Technological Physics Engineering from Instituto Superior Técnico, Technical University of Lisbon in 1999 and joined the lab of Prof. Maria Carmo-Fonseca at the Faculty of Medicine, University of Lisbon in the same year for his PhD, which was focused on the study of dynamics and interactions of nuclear proteins with quantitative photobleaching microscopy.

During his PhD, José Rino participated in an Integrated Project funded by the European Union 6th Framework Programme with the goal of generating and applying novel advanced technology for non-invasive imaging of biomolecular function in living systems. He also collaborated and trained in advanced microscopy techniques at the labs of Dr. Elisa Izaurralde and Dr. Jan Ellenberg at the European Molecular Biology Laboratory (Germany) and of Prof. Theodorus Gadella at the University of Amsterdam (The Netherlands). José completed his PhD in Biophysics in 2007 from the Faculty of Sciences, University of Lisbon, having co-authored 4 articles in international peer review journals as well as a book chapter.

José's postdoctoral research, carried out at the Instituto de Medicina Molecular (IMM) in the lab of Prof. Carmo-Fonseca, focused on applying quantitative microscopy methods to study mRNA biogenesis and resulted in 5 published articles. In 2009, José was hired under the //Ciência 2008// program by the IMM as Head of the Bioimaging Unit, the core microscope facility of the Institute. Between 2013 and 2016 José was also responsible for the Optical Imaging and Microscopy Platform of the Champalimaud Centre for the Unknown, Lisbon. In 2016 José was also appointed by the IMM as Head of the Flow Cytometry Facility, being responsible for both the Bioimaging and Flow Cytometry Facilities until 2020. 

José is currently Head of the Bioimaging Platform at the Gulbenkian Institute for Molecular Medicine. 

==== Publications ====

  * [[https://pubmed.ncbi.nlm.nih.gov/40644556/|PD-1 and ICOS are coexpressed in T follicular helper cells but define three stages of maturation of T follicular regulatory cells]], Ribeiro F, Antunes D, Pires AR, Rino J, Filipe B, Jesus K, Correia R, Fonseca VR, Kumar S, Graca L, //Sci Adv// **2025**

  * [[https://pubmed.ncbi.nlm.nih.gov/37606764/|Regeneration of starfish radial nerve cord restores animal mobility and unveils a new coelomocyte population]], Magalhães F, Andrade C, Simões B, Brigham F, Valente R, Martinez P, Rino J, Sugni M, Coelho AV, //Cell Tissue Res// **2023**
  
  * [[https://pubmed.ncbi.nlm.nih.gov/36316447/|A new protocol for whole-brain biodistribution analysis of AAVs by tissue clearing, light-sheet microscopy and semi-automated spatial quantification]], Lopes MM, Paysan J, Rino J, Lopes SM, Pereira de Almeida L, Cortes L, Nobre RJ, //Gene Ther// **2022**

  * [[https://pubmed.ncbi.nlm.nih.gov/35182478/|Gene architecture directs splicing outcome in separate nuclear spatial regions]], Tammer L, Hameiri O, Keydar I, Roy VR, Ashkenazy-Titelman A, Custódio N, Sason I, Shayevitch R, Rodríguez-Vaello V, Rino J, Maor GL, Leader Y, Khair D, Aiden EL, Elkon R, Irimia M, Sharan R, Shav-Tal Y, Carmo-Fonseca M, Ast G, //Mol Cell// **2022**

  * [[https://pubmed.ncbi.nlm.nih.gov/33815394/|Characterization of Coelomic Fluid Cell Types in the Starfish Marthasterias glacialis Using a Flow Cytometry/Imaging Combined Approach]], Andrade A, Oliveira B, Guatelli S, Martinez P, Simões B, Bispo C, Ferrario C, Bonasoro F, Rino J, Sugni M, Gardner R, Zilhão R, Coelho AV, //Front Immunol// **2021** 

  * [[https://pubmed.ncbi.nlm.nih.gov/32081879|Low doses of ionizing radiation enhance angiogenesis and consequently accelerate post-embryonic development but not regeneration in zebrafish]], Marques FG, Carvalho L, Sousa JS, Rino J, Diegues I, Poli E, Pina F, Saúde L, Santos SCR. //Sci Rep// **2020**

  * [[https://www.ncbi.nlm.nih.gov/pubmed/31442107|Low Doses of Ionizing Radiation Enhance the Angiogenic Potential of Adipocyte Conditioned Medium]], Marques FG, Poli E, Rino J, Pinto MT, Diegues I, Pina F, Santos SCR. //Radiat Res// **2019**

  * [[https://www.ncbi.nlm.nih.gov/pubmed/30888352|Lanthanide complexes with phenanthroline-based ligands: insights into cell death mechanisms obtained by microscopy techniques]], Cabral Campello MP, Palma E, Correia I, Paulo PMR, Matos A, Rino J, Coimbra J, Pessoa JC, Gambino D, Paulo A, Marques F. //Dalton Trans// **2019**

  * [[https://www.ncbi.nlm.nih.gov/pubmed/28465489|Quantification of cell cycle kinetics by EdU (5-ethynyl-2'-deoxyuridine)-coupled-fluorescence-intensity analysis]], Pereira PD, Serra-Caetano A, Cabrita M, Bekman E, Braga J, Rino J, Santus R, Filipe PL, Sousa AE, Ferreira JA. //Oncotarget// **2017**

  * [[https://www.ncbi.nlm.nih.gov/pubmed/28444128|Low-dose ionizing radiation induces therapeutic neovascularization in a pre-clinical model of hindlimb ischemia]], Ministro A, de Oliveira P, Nunes RJ, Dos Santos Rocha A, Correia A, Carvalho T, Rino J, Faísca P, Becker JD, Goyri-O'Neill J, Pina F, Poli E, Silva-Santos B, Pinto F, Mareel M, Serre K, Constantino Rosa Santos S. //Cardiovasc Res// **2017**

  * [[https://www.ncbi.nlm.nih.gov/pubmed/28324612|Quantitative Image Analysis of Single-Molecule mRNA Dynamics in Living Cells]], Rino J, de Jesus AC, Carmo-Fonseca M. //Methods Mol Biol// **2017**

  * [[https://www.ncbi.nlm.nih.gov/pubmed/26534909|Adenosine A2A Receptors Modulate α-Synuclein Aggregation and Toxicity]], Ferreira DG, Batalha VL, Vicente Miranda H, Coelho JE, Gomes R, Gonçalves FQ, Real JI, Rino J, Albino-Teixeira A, Cunha RA, Outeiro TF, Lopes LV. //Cereb Cortex// **2017**

  * [[https://www.ncbi.nlm.nih.gov/pubmed/28302904|Pharmacological inhibition of the spliceosome subunit SF3b triggers exon junction complex-independent nonsense-mediated decay]], Carvalho T, Martins S, Rino J, Marinho S, Carmo-Fonseca M. //J Cell Sci// **2017**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/26855377|STaQTool: Spot tracking and quantification tool for monitoring splicing of single pre-mRNA molecules in living cells]], Rino J, de Jesus AC, Carmo-Fonseca M. //Methods// **2016**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/26463395|Single-Molecule Live-Cell Visualization of Pre-mRNA Splicing]], Martin RM, Rino J, de Jesus AC, Carmo-Fonseca M. //Methods Mol Biol// **2016**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/26658436|Decrease of CD68 Synovial Macrophages in Celastrol Treated Arthritic Rats]], Cascão R, Vidal B, Lopes IP, Paisana E, Rino J, Moita LF, Fonseca JE. //PLoS One// **2015**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/26389938|Alterations of Nuclear Architecture and Epigenetic Signatures during African Swine Fever Virus Infection]], Simões M, Rino J, Pinheiro I, Martins C, Ferreira F. //Viruses// **2015**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/26068754|Single-Molecule Imaging of RNA Splicing in Live Cells]], Rino J, Martin RM, Carvalho C, de Jesus AC, Carmo-Fonseca M. //Methods Enzymol// **2015**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/24915168|Synthesis, characterization and biological evaluation of carboranylmethylbenzo[b]acridones as novel agents for boron neutron capture therapy]], da Silva AF, Seixas RS, Silva AM, Coimbra J, Fernandes AC, Santos JP, Matos A, Rino J, Santos I, Marques F. //Org Biomol Chem// **2014**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/24810576|Phosphorylation modulates clearance of alpha-synuclein inclusions in a yeast model of Parkinson's disease]], Tenreiro S, Reimão-Pinto MM, Antas P, Rino J, Wawrzycka D, Macedo D, Rosado-Ramos R, Amen T, Waiss M, Magalhães F, Gomes A, Santos CN, Kaganovich D, Outeiro TF. //PLoS Genet// **2014**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/24604817|Enteric mucosa integrity in the presence of a preserved innate interleukin 22 compartment in HIV type 1-treated individuals]], Fernandes SM, Pires AR, Ferreira C, Foxall RB, Rino J, Santos C, Correia L, Poças J, Veiga-Fernandes H, Sousa AE. //J Infect Dis// **2014**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/23969316|Imaging dynamic interactions between spliceosomal proteins and pre-mRNA in living cells]], Rino J, Martin RM, Carvalho T, Carmo-Fonseca M. //Methods// **2014**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/24035393|Live-cell visualization of pre-mRNA splicing with single-molecule sensitivity]], Martin RM, Rino J, Carvalho C, Kirchhausen T, Carmo-Fonseca M. //Cell Rep// **2013**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/23325844|Histone methyltransferase SETD2 coordinates FACT recruitment with nucleosome dynamics during transcription]], Carvalho S, Raposo AC, Martins FB, Grosso AR, Sridhara SC, Rino J, Carmo-Fonseca M, de Almeida SF. //Nucleic Acids Res// **2013**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/23038670|Synthesis and biological studies of pyrazolyl-diamine Pt(II) complexes containing polyaromatic DNA-binding groups]], Gama S, Mendes F, Esteves T, Marques F, Matos A, Rino J, Coimbra J, Ravera M, Gabano E, Santos I, Paulo A. //Chembiochem// **2012**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/21980525|VEGFR2 translocates to the nucleus to regulate its own transcription]], Domingues I, Rino J, Demmers JA, de Lanerolle P, Santos SC. //PLoS One// **2011**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/21892168|Spliceosome assembly is coupled to RNA polymerase II dynamics at the 3' end of human genes]], Martins SB, Rino J, Carvalho T, Carvalho C, Yoshida M, Klose JM, de Almeida SF, Carmo-Fonseca M. //Nat Struct Mol Biol// **2011**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/21706254|Nuclear targeting with cell-specific multifunctional tricarbonyl M(I) (M is Re, (99m)Tc) complexes: synthesis, characterization, and cell studies]], Esteves T, Marques F, Paulo A, Rino J, Nanda P, Smith CJ, Santos I. //J Biol Inorg Chem// **2011**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/21283118|RNA seeds nuclear bodies]], Carmo-Fonseca M, Rino J. //Nat Cell Biol// **2011**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/20648265|Tricarbonyl M(I) (M = Re, (99m)Tc) complexes bearing acridine fluorophores: synthesis, characterization, DNA interaction studies and nuclear targeting]], Esteves T, Xavier C, Gama S, Mendes F, Raposinho PD, Marques F, Paulo A, Pessoa JC, Rino J, Viola G, Santos I. //Org Biomol Chem// **2010**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/20574535|Low doses of ionizing radiation promote tumor growth and metastasis by enhancing angiogenesis]], Sofia Vala I, Martins LR, Imaizumi N, Nunes RJ, Rino J, Kuonen F, Carvalho LM, Rüegg C, Grillo IM, Barata JT, Mareel M, Santos SC. //PLoS One// **2010**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/20190194|IL-7 induces rapid clathrin-mediated internalization and JAK3-dependent degradation of IL-7Ralpha in T cells]], Henriques CM, Rino J, Nibbs RJ, Graham GJ, Barata JT. //Blood// **2010**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/19616950|The spliceosome: a self-organized macromolecular machine in the nucleus?]], Rino J, Carmo-Fonseca M. //Trends Cell Biol// **2009**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/19247949|Frontiers in fluorescence microscopy]], Rino J, Braga J, Henriques R, Carmo-Fonseca M. //Int J Dev Biol// **2009**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/18381449|Conjugation of human topoisomerase 2 alpha with small ubiquitin-like modifiers 2/3 in response to topoisomerase inhibitors: cell cycle stage and chromosome domain specificity]], Agostinho M, Santos V, Ferreira F, Costa R, Cardoso J, Pinheiro I, Rino J, Jaffray E, Hay RT, Ferreira J. //Cancer Res// **2008**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/18285458|Splicing factors SF1 and U2AF associate in extraspliceosomal complexes]], Rino J, Desterro JM, Pacheco TR, Gadella TW Jr, Carmo-Fonseca M. //Mol Cell Biol// **2008**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/18234838|Inactivation of cleavage factor I components Rna14p and Rna15p induces sequestration of small nucleolar ribonucleoproteins at discrete sites in the nucleus]], Carneiro T, Carvalho C, Braga J, Rino J, Milligan L, Tollervey D, Carmo-Fonseca M. //Mol Biol Cell// **2008**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/17967051|A stochastic view of spliceosome assembly and recycling in the nucleus]], Rino J, Carvalho T, Braga J, Desterro JM, Lührmann R, Carmo-Fonseca M. //PLoS Comput Biol// **2007**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/17403903|Depletion of the yeast nuclear exosome subunit Rrp6 results in accumulation of polyadenylated RNAs in a discrete domain within the nucleolus]], Carneiro T, Carvalho C, Braga J, Rino J, Milligan L, Tollervey D, Carmo-Fonseca M. //Mol Cell Biol// **2007**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/16750525|Studies on the role of NonA in mRNA biogenesis]], Kozlova N, Braga J, Lundgren J, Rino J, Young P, Carmo-Fonseca M, Visa N. //Exp Cell Res// **2006**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/15113082|Photobleaching microscopy reveals the dynamics of mRNA-binding proteins inside live cell nuclei]], Braga J, Rino J, Carmo-Fonseca M. //Prog Mol Subcell Biol// **2004**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/14978217|Human topoisomerase IIalpha: targeting to subchromosomal sites of activity during interphase and mitosis]], Agostinho M, Rino J, Braga J, Ferreira F, Steffensen S, Ferreira J. //Mol Biol Cell// **2004**

  * [[http://www.ncbi.nlm.nih.gov/pubmed/12473688|The intranuclear mobility of messenger RNA binding proteins is ATP dependent and temperature sensitive]], Calapez A, Pereira HM, Calado A, Braga J, Rino J, Carvalho C, Tavanez JP, Wahle E, Rosa AC, Carmo-Fonseca M. //J Cell Biol// **2002**

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