Bioimaging Wiki

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<b>Location</b>: [Oeiras] Room 0B03<br> 
<b>Manufacturer</b>: ZEISS <br>
<b>Model</b>: Stereo Lumar V.12 <br>
<b>Nickname</b>: Lumar <br>
<b>Software</b>: Micromanager (Hamamatsu camera) / uEye (uEye camera) <br>
<b>Year</b>: 2011 <br>
<b>SN</b>:  <br>
&nbsp <br>
Data will be deleted after: <b>3 months</b>
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&rarr; <a href="/facility/bioimaging/doku.php?id=oeiras_lumar_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  ZEISS Stereo Lumra.V12 Usage Statistics</a>
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===== Microscope overview =====

The Stereo Lumar V.12 is a motorized stereoscope with fluorescence, capable of imaging in colour and grayscale (camera dependent), with a zoom between 9.6x and 120x.


===== System components =====

^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Dichroic  ^  Emission  ^
|  1  |  CFP  |  Filterset 47  |  436/20  |  455  |  480/40  |
|  2  |  GFP  |  Filterset 13  |  470/20  |  495  |  505-530  |
|  3  |  TexasRed  |  Filterset 45  |  560/40  |  585  |  630/75  |
|  4  |  YFP  |  Filterset 46  |  500/20  |  515  |  535/30  |


===== Camera =====

Camera can be changed on request, and training for this can be provided if needed.
^  Model  ^  Frame Size  ^  Pixel Size (µm)  ^  Quantum Efficiency  ^
|  [[https://en.ids-imaging.com/store/ui-3370cp-rev-2.html|UI-3370CP Rev. 2 Colour]]  |  2048 × 2048  |  5.5 x 5.5  |  -  |
|  [[https://hep.hamamatsu.com/eu/en/products/C11440-42U30.html|ORCA-Flash4.0 LT+ Monochrome]]  |  2048 × 2048  |  6.5 x 6.5  |  82%  |

===== Pixel-size vs magnification =====

^  Magnification  ^  Hamamatsu (µm/pixel)  ^  IDS (µm/pixel)   ^
|    9.6x  |  10.90  |  9.40  |
|   10.0x  |  10.49  |  9.02  |
|   20.0x  |  5.07  |  4.52  |
|   30.0x  |  3.41  |  3.02  |
|   40.0x  |  2.59  |  2.26  |
|   50.0x  |  2.05  |  1.81  |
|   60.0x  |  1.73  |  1.51  |
|   70.0x  |  1.49  |  1.30  |
|   80.0x  |  1.29  |  1.13  |
|   90.0x  |  1.16  |  1.01  |
|  100.0x  |  1.04  |  0.91  |
|  110.0x  |  0.95  |  0.83  |
|  120.0x  |  0.87  |  0.76  |


===== Microscope Turn On Procedure =====
  * Turn on the **powerbar below the stone table**.
  * **If fluorescence is necessary**, turn on the **fluorescent lamp controller** on top of the desk.
===== Microscope Turn Off Procedure =====
  * Turn on the **powerbar below the stone table**.
  * **If fluorescence was used**, turn off the **fluorescent lamp controller** on top of the desk.
    * This lamp **has to be on for 30 minutes**. If you use the system less than 30 minutes, **wait** until that time to turn the lamp off, or **warn the Bioimaging Team**.



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