Bioimaging Wiki

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<b>Location</b>: [Oeiras] Room 0B02<br> 
<b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon</a> <br>
<b>Model</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series" target="_blank">Nikon Eclipse Ti</a> <br>
<b>Nickname</b>: Nikon HTM-HCS<br>
<b>Software</b>: NIS-Elements <br>
<b>Year</b>: 2015 <br>
<b>SN</b>:  <br>
&nbsp <br>
Data will be deleted after: <b>1 month</b>
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&nbsp <br>
&rarr; <a href="/facility/bioimaging/doku.php?id=nikon_htm-hcs_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Nikon HTM-HCS Quality Control</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=nikon_htm-hcs_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Nikon HTM-HCS Usage Statistics</a>
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===== Microscope overview =====

The Nikon-HTM/HCS microscope is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols. 

The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an Andor Zyla 4.2 sCMOS camera with 4.0 MPixels with a large field of view capable of maximum of 100 FPS at full frame (2048x2048). 

The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only).

===== System components =====

^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Dichroic  ^  Emission  ^
|    |    |    |    |    |    |


==== Objectives ====
^  Position  ^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  PFS compatible  ^  DIC compatible  ^  PhC compatible  ^  Reference  ^
|  1  |  10x  |  Plan Apo  |  Air  |  0.45  |  4  |  No  |  Yes (N1)  |  No  |  [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-179798|MRD70170]]  |   
|  2  |  20x  |  Plan Apo Lambda  |  Air  |  0.75  |  1  |  Yes  |  Yes (N1)  |  No  |  MRD00205*  |   
|  3  |  100x  |  Plan Apo Lambda  |  Oil  |  1.45  |  0.13  |  Yes  |  Yes (N2)  |  No  |  MRD01905*  |   
|  4  |  40x  |  Plan Fluor  |  Oil  |  1.3  |  0.24  |  Yes  |  Yes (N2)  |  No  |  [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]]  |   
|  5  |  40x  |  Plan Apo  |  Air  |  0.95  |  0.17-0.25  |  Yes  |  Yes (N2)  |  No  |  MRD00400*  |   
|  6  |  40x  |  LWD* *  |  Air  |  0.55  |  2.1  |  No  |  No  |  Yes  |  [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1683|MRP46402]]  |   

* Not fabricated anymore ; * * LWD - Long Working Distance  
==== Camera ====


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[[resources|Back to the Equipment page]]
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