Bioimaging Wiki

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<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new_p2a24.png">[Lisbon] Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> 
<b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br>
<b>Model</b>: Eclipse Ti<br>
<b>Nickname</b>: "006"<br>
<b>Software</b>: NIS-Elements AR 5.42.05<br>
<b>Year</b>: 2010 <br>
<b>SN</b>: 602805 <br>
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Data will be deleted after: <b>3 months</b>
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&rarr; <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Nikon Eclipse Ti Quality Control</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=nikon_eclipse_ti_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Nikon Eclipse Ti Usage Statistics</a>
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===== Microscope overview =====
{{  :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply, and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with an Andor Neo 5.5 monochrome cooled sCMOS camera. 

  * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]]
  * **Camera**: [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]]

This microscope is also equipped with a [[https://www.ionoptix.com/products/systems/calcium-and-contractility-system/|Ionoptix Calcium and Contractility system]] for high-speed simultaneous acquisition of Fura-2 fluorescence with video-based cell length measurement to study myocyte function, contracting spontaneously or with field stimulation.

{{::ionoptix_realtime-acquisition-cell-length-600x500.jpg?200  |}}
  * **High-speed contractility camera**: [[https://www.ionoptix.com/products/components/sensors/myocam-s3/|MyoCam-S3]]
  * **Electrical Field Stimulator**: [[https://www.ionoptix.com/products/components/stimulators/myopacer-cell-stimulator/|MyoPacer]]
  * **LED Light Source**: [[https://www.ionoptix.com/products/components/light-sources/led-light-source/|UV LEDs for Fura-2 excitation]]
  * **High-speed PMT**: [[https://www.ionoptix.com/products/components/sensors/pmt-sub-system/|PMT400]] for photon counting at 1kHz
  * **Software**: [[https://www.ionoptix.com/products/software/ionwizard-core-and-analysis/|IonWizard]] 7.7.3.180

{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.

===== System components =====

==== Filtersets Turret 1 (top) ====

^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Dichroic  ^  Emission  ^
|  1  |  Empty  |||||
|  2  |  DAPI  |  MBE41300  |  340-380 nm  |  400 nm  |  435-485 nm  |
|  3  |  FITC  |  MBE44720  |  465-495 nm  |  505 nm  |  515-555 nm  |
|  4  |  TRITC  |  MBE45600  |  528-553 nm  |  565 nm  |  590-650 nm  |
|  5  |  Cy5  |  2SK4CY50  |  608-648 nm  |  660 nm  |  672-712 nm  |
|  6  |  Empty  |||||

==== Filtersets Turret 2 (bottom) ====

^  Position  ^  Filterset  ^
|  1  |  Empty  |
|  2  |  Empty  |
|  3  |  Empty  |
|  4  |  Empty  |
|  5  |  Empty  |
|  6  |  Fura-2  |

==== Objectives ====
^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  Reference  ^
|  10x  |  CFI Plan Fluor DLL Ph1  |  Air  |  0.30  |  16.00  |   [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]]  |
|  20x  |  CFI S Plan Fluor ELWD ADM Ph1  |  Air  |  0.45  |  6.90 - 8.20  |  [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1813|MRH48230]]  |
|  40x  |  CFI CFI Plan Apochromat Lambda D  |  Air  |  0.95  |  0.21  |  [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179806|MRH70470]]  |
|  40x  |  CFI S Plan Fluor ELWD ADM Ph2  |  Air  |  0.60  |  2.80 - 3.60  |  [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]]  |
|  60x  |  Plan Apo DM Ph3  |  Oil  |  1.40  |  0.13  |  |
|  100x  |  Plan Apo DM Ph3  |  Oil  |  1.40  |  0.13  |  |

**Upon request:**
^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  Reference  ^
|  100x  |  CFI Plan  |  Water  |  1.10  |  2.50  |  [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1956|MRL07920]]  |
==== Camera ====
^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
|  [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]]  |  CCD  |  2560 x 2160  |  6.5 x 6.5  |

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