Bioimaging Wiki

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<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_map_new.png">[Lisbon] Room P3-A-16b</a><br> 
<b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br>
<b>Model</b>: DM2500<br>
<b>Nickname</b>: "DM2500"<br>
<b>Software</b>: Leica Application Suite (LAS)<br>
<b>Year</b>: 2006 <br>
<b>SN</b>: 285533-102006<br>
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Data will be deleted after: <b>3 months</b>
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&rarr; <a href="/facility/bioimaging/doku.php?id=leica_dm2500_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Leica DM2500 Usage Statistics</a>
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===== Microscope overview =====
The Leica DM2500 is an upright //brightfield microscope// equipped with a color camera ([[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450/|Leica DFC450]]) for brightfield and differential interference contrast (DIC) imaging.

{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
===== System components =====

==== Objectives ====
^  Magnification  ^  Model  ^  Type  ^  NA  ^  WD (mm)  ^  pixel size (μm)  ^  10 μm =  ^  Reference  ^
|  1.25x  |  HCX PL FLUOTAR  |  Dry  |  0.04  |  3.70  |  4.90  |  2.0 pixels  |  506215  |
|  5x  |  N PLAN  |  Dry  |  0.12  |  14.00  |  1.23  |  8.1 pixels  |  506302  |
|  10x  |  HC PL FLUOTAR  |  Dry  |  0.30  |  11.00  |  0.61  |  16.2 pixels  |  506507  |
|  20x  |  HC PL FLUOTAR  |  Dry  |  0.50  |   1.15  |  0.30  |  32.5 pixels  |  506506  |
|  40x  |  HCX PL FLUOTAR  |  Dry  |  0.75  |  0.40  |  0.15  |  65 pixels  |  506144  |
|  100x  |  HCX PL FLUOTAR  |  Oil  |  1.30  |  0.22  |  0.06  |  162.5 pixels  |  506195  |

*** pixel size for 2560 x 1920 images (Full Frame)**
==== Camera ====
^  Model  ^  Type  ^  Frame Size  ^  Pixel Size (µm)  ^
|  [[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]]  |  color CCD  |  2560 × 1920  |  3.4 x 3.4  |

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