Bioimaging Wiki

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<b>Location</b>: [Oeiras] Room 0B05<br> 
<b>Manufacturer</b>: Andor<br>
<b>Model</b>: Revolution WD<br>
<b>Scanhead</b>: <a href="https://www.yokogawa.com/eu/solutions/products-and-services/life-science/spinning-disk-confocal/#Details__Technology" target="_blank">Yokogowa W1 <a/><br>
<b>Nickname</b>: "W1"<br>
<b>Software</b>: Metamorph or IQ<br>
<b>Year</b>:  2013<br>
<b>SN</b>:  <br>
&nbsp <br>
Data will be deleted after: <b>1 month</b>
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&rarr; <a href="/facility/bioimaging/doku.php?id=andor_w1_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Andor Spinning Disk W1 Quality Control</a>
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&rarr; <a href="/facility/bioimaging/doku.php?id=andor_w1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png">  Andor Spinning Disk W1 Usage Statistics</a>
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===== Microscope overview =====

The Andor Spinning W1 system is a spinning disk laser confocal microscope with a sensitive EMCCD camera suitable for fast in-vivo imaging. It's one of the fastest and most sensitive system at the facility. The system can also scan multiple positions in the sample, with multiple channels in time-lapse mode. In order to stay in focus it also includes a perfect focus system to correct for vertical drift (Nikon's "PFS") during long movies.
Note that this system does not have widefield fluorescence, so samples can only be previewed in brightfield mode.

===== System components =====

==== Lasers =====

^  Type  ^  Excitation line  ^  Nominal power  ^
|  Diode405  |  405nm  |  100mW  |
|  DPSS488  |  488nm  |  100mW  |
|  DPSS561  |  561nm  |  50mW  |
|  Diode640  |  640nm  |  100mW  |

==== Objectives ====

^  Magnification  ^  Model  ^  Immersion  ^  NA  ^  WD (mm)  ^  PFS compatible  ^  DIC compatible  ^  PhC compatible  ^  Reference  ^
|   4x  |  Nikon Plan DL   |  -  |  0.13  |  16.5  |  N  |  N/A  |  Ph1  |  [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1834|MRH20045]]  |   
|  10x  |  Nikon Plan DL  |  -  |  0.25  |  10.5  |  Yes  |  Yes (N1)  |  Ph1  |  [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1701|MRL20102]]  |   
|  20x  |  Nikon Plan Fluor  |  -  |  0.5  |  2.1  |  Yes  |  No  |  Ph1  |  [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1837|MRH10205]]  |   
|  20x  |  Nikon Plan Fluor  |  MImm  |  0.75  |  0.35  |  Yes  |  Yes (N1)  |  No  |  [[https://|MRDxxxxx]]  |   
|  40x  |  Plan Fluor  |  Oil  |  1.3  |  0.24  |  Yes  |  Yes (N2)  |  No  |  [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]]  |   
|  63x  |  Nikon Plan APO VC  |  W  |  1.20  |  0.175  |  No  |  No  |  Yes  |  [[https://|MRDxxxxx]]  |   

* Not fabricated anymore ; * * LWD - Long Working Distance  

==== CSU Dichroics ====

^  Position  ^  Name/reflection  ^  Transmission  ^  
|  1  |  [[https://www.idex-hs.com/store/product-detail/ff685_di02_25x36/fl-007099|IR mirror]]  |  685LP  |
|  2  |  [[https://www.idex-hs.com/store/product-detail/di01_t405_488_568_647_13x15x0_5/fl-006984|LD 405/488/561/640]]  |  Quad mirror 80-85% transmission|
|  3  |  [[https://www.chroma.com/products/parts/zt405-488-561-640rpc|ZT 405/488/561/640]]  |  Quad mirror >95% transmission |


==== Filters (CSU Filterwheel) ====

^  Position  ^  Name/Description  ^  Transmission  ^  Dichroic  ^
|  1  |  [[http://www.semrock.com/FilterDetails.aspx?id=FF01-440/521/607/700-25|Quad Semrock]]  |  440/521/607/700-25  |  2 or 3[HE]  |
|  2  |  [[http://www.semrock.com/FilterDetails.aspx?id=FF02-447/60-25|DAPI Semrock]]  |  447/60  |  2 or 3[HE]  |
|  3  |  [[http://www.semrock.com/FilterDetails.aspx?id=FF01-525/30-25|GFP Semrock]]  |  525/30  |  2 or 3[HE]  |
|  4  |  [[http://www.semrock.com/FilterDetails.aspx?id=FF01-607/36-25|RFP Semrock]]  |  607/36  |  2 or 3[HE]  |
|  5  |  [[http://www.semrock.com/FilterDetails.aspx?id=FF02-685/40-25|Cy5 Semrock]]  |  685/40  |  2 or 3[HE]  |
|  6  |  Empty  |  -  |  -  |
|  7  |  [[http://www.chroma.com/product/individual-filters-mirrors/widefield-microscopy/ET655lp-EM|Cy5LP Chroma HE]]  |  655 LP  |  2 or 3[HE]  |
|  8  |  [[http://www.chroma.com/product/individual-filters-mirrors/widefield-microscopy/ET595_x_50m-EM|RFP Chroma HE]]   |  595/50  |  2 or 3[HE]  |
|  9  |  [[http://www.chroma.com/product/individual-filters-mirrors/widefield-microscopy/ET525_x_50m-EM|GFP Chroma HE]]  |  525/50  |  2 or 3[HE]  |
|  10  |  [[http://www.chroma.com/product/individual-filters-mirrors/widefield-microscopy/ET450_x_50m-EM|DAPI Chroma HE]]  |  450/50  |  2 or 3[HE]  |


==== Camera(s) ====

^  Model/type  ^  Chip Size  ^  Dexel Size   ^  Quantum Efficiency  ^
|  [[https://andor.oxinst.com/products/ixon-emccd-camera-series/ixon-ultra-888|Andor iXon 888 ]]  |  1024 × 1024  |  13 um  |  >95%  |
|  [[https://andor.oxinst.cn/assets/uploads/products/andor/documents/Zyla-4.2-Specifications-Life-Science.pdf|Andor Zyla 4.2 PLUS /sCMOS]]  |  2048 × 2048  |  6.5 um  |  82%  |
|  [[https://en.ids-imaging.com/store/ui-3370cp-rev-2.html|IDS Monochrome UI-3370CP-M-GL]]  |  2048 × 2048  |  5.5 um  |  60%  |

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