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zeiss_lsm_980-32 [2025/12/08 21:26] bioimaging [Filtersets (for eyepieces)] |
zeiss_lsm_980-32 [2026/04/16 13:36] (current) bioimaging |
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| <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html">LSM 980 with Airyscan 2</a><br> | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html">LSM 980 with Airyscan 2</a><br> | ||
| <b>Nickname</b>: "980-32"<br> | <b>Nickname</b>: "980-32"<br> | ||
| - | <b>Software</b>: ZEN 3.12<br> | + | <b>Software</b>: ZEN 3.13<br> |
| <b>Year</b>: 2024 <br> | <b>Year</b>: 2024 <br> | ||
| <b>SN</b>: 2842001157 <br> | <b>SN</b>: 2842001157 <br> | ||
| Line 43: | Line 43: | ||
| <html> | <html> | ||
| - | <p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/Ji_PVld77Gs" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> | + | <p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/Ji_PVld77Gs?si=_UOe1Bq3f2K6Hb9-" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" referrerpolicy="strict-origin-when-cross-origin" allowfullscreen></iframe></p> |
| </html> | </html> | ||
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| ^ LASER ^ Wavelength ^ Maximum Power ^ | ^ LASER ^ Wavelength ^ Maximum Power ^ | ||
| - | | violet | <fc #a600ff>405 nm</fc> | 15 mW | | + | | Violet | <fc #a600ff>405 nm</fc> | 15 mW | |
| - | | blue-violet | <fc #0028ff>445 nm</fc> | 10 mW | | + | | Blue-Violet | <fc #0028ff>445 nm</fc> | 10 mW | |
| - | | blue | <fc #00f7ff>488 nm</fc> | 13 mW | | + | | Blue | <fc #00f7ff>488 nm</fc> | 13 mW | |
| - | | green | <fc #1aff00>514 nm</fc> | 13 mW | | + | | Green | <fc #1aff00>514 nm</fc> | 13 mW | |
| - | | yellow-green | <fc #c6ff00>561 nm</fc> | 13 mW | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 13 mW | |
| - | | orange | <fc #ffd200>594 nm</fc> | 4 mW | | + | | Orange | <fc #ffd200>594 nm</fc> | 4 mW | |
| - | | red | <fc #ff2600>639 nm</fc> | 10 mW | | + | | Red | <fc #ff2600>639 nm</fc> | 10 mW | |
| ==== LEDs (for eyepieces) ==== | ==== LEDs (for eyepieces) ==== | ||
| Line 72: | Line 72: | ||
| | G | <fc #b3ff00>555 nm</fc> | | | G | <fc #b3ff00>555 nm</fc> | | ||
| | R | <fc #ff4f00>630 nm</fc> | | | R | <fc #ff4f00>630 nm</fc> | | ||
| + | |||
| + | ==== Filterset (for eyepieces) ==== | ||
| + | |||
| + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ||
| + | | 1 | Blue | G 365 nm | 395 nm | <fc #0028ff>420-470 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488049-9901-400|FS49]] | | ||
| + | | 2 | Green | 450-490 nm | 495 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/sa/fluorescenceAssistant/filtersets/489038-9901-400|FS38HE]] | | ||
| + | | 3 | Orange-Red | 538-563 nm | 570 nm | <fc #ffad00>570-640 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489043-9901-400|FS43]] | | ||
| + | | 5 | QUAD | 370-400 nm | 405 nm | <fc #5400ff>410-440 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489090-9110-400| FS90HE]] | | ||
| + | | ::: | ::: | 450-488 nm | 493 nm | <fc #1fff00>499-529 nm</fc> | ::: | | ||
| + | | ::: | ::: | 540-570 nm | 575 nm | <fc #ffd900>580-605 nm</fc> | ::: | | ||
| + | | ::: | ::: | 615-648 nm | 653 nm | <fc #ef0000>659-759 nm</fc> | ::: | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
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| | <fc #01b3f5>40x</fc> | LD LCI Plan-Apochromat Corr | Water | 1.20 | 0.41 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420862-9970-799/Objective-LD-LCI-Plan-Apochromat-40x-1.2-Imm-Corr-DIC-M27|420862-9970-799]] | | | <fc #01b3f5>40x</fc> | LD LCI Plan-Apochromat Corr | Water | 1.20 | 0.41 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420862-9970-799/Objective-LD-LCI-Plan-Apochromat-40x-1.2-Imm-Corr-DIC-M27|420862-9970-799]] | | ||
| | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | ||
| - | |||
| - | ==== Filtersets (for eyepieces) ==== | ||
| - | |||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
| - | | 1 | QUAD | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000| FS90HE]] | <fc #be00cc>370-400 nm</fc> | <fc #a600ff>405 nm</fc> | <fc #5400ff>410-440 nm</fc> | | ||
| - | | ::: | ::: | ::: | <fc #00a9ff>450-488 nm</fc> | <fc #00ffe0>493 nm</fc> | <fc #1fff00>499-529 nm</fc> | | ||
| - | | ::: | ::: | ::: | <fc #b3ff00>540-570 nm</fc> | <fc #f0ff00>575 nm</fc> | <fc #ffd900>580-605 nm</fc> | | ||
| - | | ::: | ::: | ::: | <fc #ff4b00>615-648 nm</fc> | <fc #ff0000>653 nm</fc> | <fc #ef0000>659-759 nm</fc> | | ||
| ===== How to start your session ===== | ===== How to start your session ===== | ||
| If you are the **first user** of the day: | If you are the **first user** of the day: | ||
| - | * Turn off Main Switch | ||
| - | {{::zeiss_lsm_980_main_switch.png?100|}} | ||
| * Turn off Components | * Turn off Components | ||
| {{::zeiss_lsm_980_components.png?100|}} | {{::zeiss_lsm_980_components.png?100|}} | ||
| + | * Turn off Main Switch | ||
| + | {{::zeiss_lsm_980_main_switch.png?100|}} | ||
| * Turn on the computer | * Turn on the computer | ||
| * Turn on Main Switch | * Turn on Main Switch | ||
zeiss_lsm_980-32.1765225610.txt.gz · Last modified: 2025/12/08 21:26 by bioimaging
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