zeiss_lsm_980-32
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zeiss_lsm_980-32 [2025/12/08 17:25] bioimaging |
zeiss_lsm_980-32 [2025/12/12 15:09] (current) bioimaging [LASERs] |
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| ^ LASER ^ Wavelength ^ Maximum Power ^ | ^ LASER ^ Wavelength ^ Maximum Power ^ | ||
| - | | <fc #8200c8>405 (violet)</fc> | 405 nm | 15 mW | | + | | Violet | <fc #a600ff>405 nm</fc> | 15 mW | |
| - | | <fc #0028ff>445 (blue violet)</fc> | 445 nm | 10 mW | | + | | Blue-Violet | <fc #0028ff>445 nm</fc> | 10 mW | |
| - | | <fc #00f7ff>488 (blue)</fc> | 488 nm | 13 mW | | + | | Blue | <fc #00f7ff>488 nm</fc> | 13 mW | |
| - | | <fc #1aff00>514 (green)</fc> | 514 nm | 13 mW | | + | | Green | <fc #1aff00>514 nm</fc> | 13 mW | |
| - | | <fc #c6ff00>561 (yellow-green)</fc> | 561 nm | 13 mW | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 13 mW | |
| - | | <fc #ffd200>594 (orange)</fc> | 594 nm | 4 mW | | + | | Orange | <fc #ffd200>594 nm</fc> | 4 mW | |
| - | | <fc #ff2600>639 (red)</fc> | 639 nm | 10 mW | | + | | Red | <fc #ff2600>639 nm</fc> | 10 mW | |
| + | |||
| + | ==== LEDs (for eyepieces) ==== | ||
| + | |||
| + | ^ Line ^ Wavelength ^ | ||
| + | | UV | <fc #be00cc>385 nm</fc> | | ||
| + | | B | <fc #00c0ff>475 nm</fc> | | ||
| + | | G | <fc #b3ff00>555 nm</fc> | | ||
| + | | R | <fc #ff4f00>630 nm</fc> | | ||
| + | |||
| + | ==== Filterset (for eyepieces) ==== | ||
| + | |||
| + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ||
| + | | 1 | QUAD | 370-400 nm | 405 nm | <fc #5400ff>410-440 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000| FS90HE]] | | ||
| + | | ::: | ::: | 450-488 nm | 493 nm | <fc #1fff00>499-529 nm</fc> | ::: | | ||
| + | | ::: | ::: | 540-570 nm | 575 nm | <fc #ffd900>580-605 nm</fc> | ::: | | ||
| + | | ::: | ::: | 615-648 nm | 653 nm | <fc #ef0000>659-759 nm</fc> | ::: | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| + | |||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | <fc #e94543>5x</fc> | EC Plan-NeoFluar | Air | 0.16 | 13.6 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] | | + | | <fc #df2b37>5x</fc> | EC Plan-NeoFluar | Air | 0.16 | 13.6 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] | |
| | <fc #0aa05c>20x</fc> | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | | | <fc #0aa05c>20x</fc> | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | | ||
| | <fc #01b3f5>40x</fc> | LD LCI Plan-Apochromat Corr | Water | 1.20 | 0.41 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420862-9970-799/Objective-LD-LCI-Plan-Apochromat-40x-1.2-Imm-Corr-DIC-M27|420862-9970-799]] | | | <fc #01b3f5>40x</fc> | LD LCI Plan-Apochromat Corr | Water | 1.20 | 0.41 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420862-9970-799/Objective-LD-LCI-Plan-Apochromat-40x-1.2-Imm-Corr-DIC-M27|420862-9970-799]] | | ||
| | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | ||
| - | |||
| - | ==== LEDs (Ocular) ==== | ||
| - | ^ Line ^ Wavelength ^ | ||
| - | | UV | 385 nm | | ||
| - | | B | 475 nm | | ||
| - | | G | 555 nm | | ||
| - | | R | 630 nm | | ||
| - | |||
| - | ==== Filterset (Ocular) ==== | ||
| - | |||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
| - | | 1 | QUAD | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000| FS90HE]] | 370-400 nm | 405 nm | 410-440 nm | | ||
| - | | ::: | ::: | ::: | 450-488 nm | 493 nm | 499-529 nm | | ||
| - | | ::: | ::: | ::: | 540-570 nm | 575 nm | 580-605 nm | | ||
| - | | ::: | ::: | ::: | 615-648 nm | 653 nm | 659-759 nm | | ||
| ===== How to start your session ===== | ===== How to start your session ===== | ||
| - | The system should be always on, you only need to: | + | If you are the **first user** of the day: |
| - | * Turn on the computer (if needed) | + | * Turn off Components |
| + | {{::zeiss_lsm_980_components.png?100|}} | ||
| + | * Turn off Main Switch | ||
| + | {{::zeiss_lsm_980_main_switch.png?100|}} | ||
| + | * Turn on the computer | ||
| + | * Turn on Main Switch | ||
| + | * Turn on Components | ||
| + | * Log into Windows with your Agendo credentials | ||
| + | * Start **ZEN** | ||
| + | Else: | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| * Start **ZEN** | * Start **ZEN** | ||
| Line 96: | Line 107: | ||
| ===== How to end your session ===== | ===== How to end your session ===== | ||
| - | If there is another user for this microscope in the __next hour__: | + | If there is another user: |
| - | * Exit the ZEN software, leave the lasers on | + | |
| - | * Log off the computer | + | |
| - | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | + | |
| - | Else: | + | |
| - | * Turn off the lasers on **ZEN** | + | |
| * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| * Exit the ZEN software | * Exit the ZEN software | ||
| * Log off the computer | * Log off the computer | ||
| - | + | If you are the **last user** of the day: | |
| - | ===== Microscope Turn On Procedure (after complete shutdown) ===== | + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) |
| - | + | ||
| - | If the system is completely off, please follow this procedure: | + | |
| - | + | ||
| - | * Turn on the ''MAIN SWITCH'' | + | |
| - | {{::zeiss_lsm_980_main_switch.png?100|}} | + | |
| - | * Turn on the ''COMPONENTS'' switch | + | |
| - | {{::zeiss_lsm_980_components.png?100|}} | + | |
| - | * Turn on the Laser key | + | |
| - | * Turn on the computer | + | |
| - | * Log into Windows with your Agendo credentials | + | |
| - | * Start the **ZEN** software | + | |
| - | + | ||
| - | ===== Microscope Turn Off Procedure (for complete shutdown) ===== | + | |
| - | + | ||
| - | Please only shutdown the system if instructed to do so by the Bioimaging Staff. | + | |
| - | + | ||
| - | * Turn off the lasers on **ZEN** | + | |
| * Exit the ZEN software | * Exit the ZEN software | ||
| - | * Shutdown the computer | + | * Turn off the computer |
| - | * Turn off the Laser key | + | |
| - | * Turn off the ''COMPONENTS'' switch | + | |
| - | * Turn off the ''MAIN SWITCH'' | + | |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lsm_980-32.1765211101.txt.gz · Last modified: 2025/12/08 17:25 by bioimaging
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