zeiss_lsm_980-32
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zeiss_lsm_980-32 [2024/07/16 23:45] bioimaging |
zeiss_lsm_980-32 [2025/07/29 16:57] (current) bioimaging [Booking Rules] |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_980-32_name.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_zeiss_lsm_980-32_with_airyscan_2_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_980-32_wiki.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_980-32_wiki.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_980-32_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_980-32_wiki_2024.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_980-32_map_2024.png">Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lsm_980-32_map_2024.png">[Lisbon] Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html">LSM 980 with Airyscan 2</a><br> | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html">LSM 980 with Airyscan 2</a><br> | ||
| <b>Nickname</b>: "980-32"<br> | <b>Nickname</b>: "980-32"<br> | ||
| - | <b>Software</b>: ZEN 3.8 (blue edition)<br> | + | <b>Software</b>: ZEN 3.12<br> |
| <b>Year</b>: 2024 <br> | <b>Year</b>: 2024 <br> | ||
| <b>SN</b>: 2842001157 <br> | <b>SN</b>: 2842001157 <br> | ||
|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
| - |   <br> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :confocal_pinhole.png?340}} The Zeiss LSM 980-32 with Airyscan 2 is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a dark large cage incubator and a stage top incubator for temperature control and CO2 supply, with Definite Focus.3 for hardware focus control during long time-lapse acquisitions, and with an [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research/autoimmersion-module.html|autoimmersion module for water objectives]]. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions, using the Tiles Advanced Setup of ZEN blue. It is equipped with [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/ai-sample-finder.html|AI Sample Finder]] for automated sample placement, focus adjustment and identification of the relevant sample areas. Its [[http://www.zeiss.com/content/dam/Microscopy/Products/laser-scan/LSM%20880/zeiss-lsm880-airyscan-principle-672.jpg|Airyscan 2]] with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (120 nm laterally and 350 nm axially in SR mode, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=tUiEa7vcggQ|Multiplex Mode]], which enables parallel excitation and detection in 4Y or 8Y modes (speed improvement of 4x/8x maintaining Airyscan sensitivity). Its scanning unit further includes a [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=32-channel_quasar.png|32-channel GaAsP detector]] for simultaneous [[https://zeiss-campus.magnet.fsu.edu/articles/spectralimaging/introduction.html|spectral imaging and separation of fluorescent labels]] and two PMT detectors. It is equipped with lasers from violet to far red (405, 445, 488, 514, 561, 594 and 639 nm excitation wavelengths). Its[[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|LSM Plus deconvolution]] mode enhances signal-to-noise ratio by acting as a denoising step post image acquisition, allowing for high acquisition speed and low laser power. Using its [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|Airyscan Joint Deconvolution (jDCV)]] option, the resolution can be improved to 90 nm (XY) and 270 nm (Z). A dedicated workstation is also available for Direct Processing during acquisition. | + | {{ :confocal_pinhole.png?340}} The Zeiss LSM 980-32 with Airyscan 2 is a [[https://www.youtube.com/watch?v=rTkjQ6Q0p5I|point scanning confocal microscope]] able to generate high-resolution three-dimensional images of thick specimens with high sensitivity, high speed and low photodamage. It is an inverted microscope especially suitable for live cell imaging and photobleaching experiments, equipped with a dark large cage incubator and a stage top incubator for temperature control and CO2 supply, with Definite Focus.3 for hardware focus control during long time-lapse acquisitions, and with an [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research/autoimmersion-module.html|autoimmersion module for water objectives]]. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions, using the Tiles Advanced Setup of ZEN blue. It is equipped with [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/ai-sample-finder.html|AI Sample Finder]] for automated sample placement, focus adjustment and identification of the relevant sample areas. Its Airyscan 2 with 32 concentrically arranged GaAsP detection elements is able to collect all light from an Airy pattern simultaneously, allowing for increased resolution (120 nm laterally and 350 nm axially in SR mode, at 488 nm). It can also be used in [[https://www.youtube.com/watch?v=tUiEa7vcggQ|Multiplex Mode]], which enables parallel excitation and detection in 4Y or 8Y modes (speed improvement of 4x/8x maintaining Airyscan sensitivity). Its scanning unit further includes a [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=32-channel_quasar.png|32-channel GaAsP detector]] for simultaneous [[https://zeiss-campus.magnet.fsu.edu/articles/spectralimaging/introduction.html|spectral imaging and separation of fluorescent labels]] and two PMT detectors. It is equipped with lasers from violet to far red (405, 445, 488, 514, 561, 594 and 639 nm excitation wavelengths). Its[[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|LSM Plus deconvolution]] mode enhances signal-to-noise ratio by acting as a denoising step post image acquisition, allowing for high acquisition speed and low laser power. Using its [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|Airyscan Joint Deconvolution (jDCV)]] option, the resolution can be improved to 90 nm (XY) and 270 nm (Z). A dedicated workstation is also available for Direct Processing during acquisition. |
| * **Microscope**: [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research.html|Zeiss Axio Observer]] | * **Microscope**: [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research.html|Zeiss Axio Observer]] | ||
| Line 49: | Line 48: | ||
| * [[https://www.youtube.com/watch?v=zA4xqq-EL9g|ZEISS Webinar: LSM 980 Airyscan 2 Multiplex mode]] | * [[https://www.youtube.com/watch?v=zA4xqq-EL9g|ZEISS Webinar: LSM 980 Airyscan 2 Multiplex mode]] | ||
| - | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| - | ===== Booking Rules ===== | ||
| - | - **Users** can book at maximum **8 hours per week** | ||
| - | - This usage restriction does not apply for **weekends** and for working days **before 9:00** and **after 21:00** | ||
| - | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | ||
| ===== System components ===== | ===== System components ===== | ||
| Line 93: | Line 89: | ||
| The system should be always on, you only need to: | The system should be always on, you only need to: | ||
| - | * Turn on the computer | + | * Turn on the computer (if needed) |
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| * Start **ZEN** | * Start **ZEN** | ||
| Line 102: | Line 98: | ||
| * Exit the ZEN software, leave the lasers on | * Exit the ZEN software, leave the lasers on | ||
| * Log off the computer | * Log off the computer | ||
| - | * Clean up immersion objectives | + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) |
| Else: | Else: | ||
| * Turn off the lasers on **ZEN** | * Turn off the lasers on **ZEN** | ||
| - | * Clean up immersion objectives | + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) |
| * Exit the ZEN software | * Exit the ZEN software | ||
| - | * Shutdown the computer | + | * Log off the computer |
| ===== Microscope Turn On Procedure (after complete shutdown) ===== | ===== Microscope Turn On Procedure (after complete shutdown) ===== | ||
| Line 117: | Line 113: | ||
| * Turn on the ''COMPONENTS'' switch | * Turn on the ''COMPONENTS'' switch | ||
| {{::zeiss_lsm_980_components.png?100|}} | {{::zeiss_lsm_980_components.png?100|}} | ||
| + | * Turn on the Laser key | ||
| * Turn on the computer | * Turn on the computer | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| Line 123: | Line 120: | ||
| ===== Microscope Turn Off Procedure (for complete shutdown) ===== | ===== Microscope Turn Off Procedure (for complete shutdown) ===== | ||
| - | Please only shutdown the system if instructed to do so by the Bioimaging Unit. | + | Please only shutdown the system if instructed to do so by the Bioimaging Staff. |
| * Turn off the lasers on **ZEN** | * Turn off the lasers on **ZEN** | ||
| * Exit the ZEN software | * Exit the ZEN software | ||
| * Shutdown the computer | * Shutdown the computer | ||
| - | * Turn off the ''COMPONENTS'' switch (do not turn off the Laser key!) | + | * Turn off the Laser key |
| + | * Turn off the ''COMPONENTS'' switch | ||
| * Turn off the ''MAIN SWITCH'' | * Turn off the ''MAIN SWITCH'' | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lsm_980-32.1721166311.txt.gz · Last modified: 2024/07/16 23:45 by bioimaging
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