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zeiss_lsm_980 [2025/07/29 16:55] bioimaging [Booking Rules] |
zeiss_lsm_980 [2026/04/16 13:50] (current) bioimaging |
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| <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html">LSM 980 with Airyscan 2</a><br> | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-980-with-airyscan-2.html">LSM 980 with Airyscan 2</a><br> | ||
| <b>Nickname</b>: "980"<br> | <b>Nickname</b>: "980"<br> | ||
| - | <b>Software</b>: ZEN 3.12<br> | + | <b>Software</b>: ZEN 3.13<br> |
| <b>Year</b>: 2021 <br> | <b>Year</b>: 2021 <br> | ||
| <b>SN</b>: 2841000239 <br> | <b>SN</b>: 2841000239 <br> | ||
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| <html> | <html> | ||
| - | <p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/Ji_PVld77Gs" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> | + | <p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/Ji_PVld77Gs?si=7rxrZElaYQGy4v5e" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" referrerpolicy="strict-origin-when-cross-origin" allowfullscreen></iframe></p> |
| </html> | </html> | ||
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| ==== LASERs ==== | ==== LASERs ==== | ||
| - | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| - | | 405 | 405 nm | 15 mW | | ||
| - | | 488 | 488 nm | 13 mW | | ||
| - | | 561 | 561 nm | 13 mW | | ||
| - | | 639 | 639 nm | 10 mW | | ||
| - | ==== Objectives ==== | + | ^ LASER ^ Wavelength ^ Maximum Power ^ |
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | | Violet | <fc #a600ff>405 nm</fc> | 15 mW | |
| - | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | + | | Blue | <fc #00f7ff>488 nm</fc> | 13 mW | |
| - | | 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 13 mW | |
| - | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | + | | Red | <fc #ff2600>639 nm</fc> | 10 mW | |
| - | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | + | |
| + | ==== LEDs (for eyepieces) ==== | ||
| - | ==== LEDs (Ocular) ==== | ||
| ^ Line ^ Wavelength ^ | ^ Line ^ Wavelength ^ | ||
| - | | UV | 385 nm | | + | | UV | <fc #be00cc>385 nm</fc> | |
| - | | B | 475 nm | | + | | B | <fc #00c0ff>475 nm</fc> | |
| - | | G | 555 nm | | + | | G | <fc #b3ff00>555 nm</fc> | |
| - | | R | 630 nm | | + | | R | <fc #ff4f00>630 nm</fc> | |
| - | ==== Filterset (Ocular) ==== | + | ==== Filterset (for eyepieces) ==== |
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ |
| - | | 1 | QUAD | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000| FS90HE]] | 370-400 nm | 405 nm | 410-440 nm | | + | | 1 | QUAD | 370-400 nm | 405 nm | <fc #5400ff>410-440 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489090-9110-400| FS90HE]] | |
| - | | ::: | ::: | ::: | 450-488 nm | 493 nm | 499-529 nm | | + | | ::: | ::: | 450-488 nm | 493 nm | <fc #1fff00>499-529 nm</fc> | ::: | |
| - | | ::: | ::: | ::: | 540-570 nm | 575 nm | 580-605 nm | | + | | ::: | ::: | 540-570 nm | 575 nm | <fc #ffd900>580-605 nm</fc> | ::: | |
| - | | ::: | ::: | ::: | 615-648 nm | 653 nm | 659-759 nm | | + | | ::: | ::: | 615-648 nm | 653 nm | <fc #ef0000>659-759 nm</fc> | ::: | |
| + | |||
| + | ==== Objectives ==== | ||
| + | |||
| + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| + | | <fc #fef042>10x</fc> | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | ||
| + | | <fc #0aa05c>20x</fc> | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | | ||
| + | | <fc #01b3f5>40x</fc> | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | ||
| + | | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | ||
| ===== How to start your session ===== | ===== How to start your session ===== | ||
| - | The system should be always on, you only need to: | + | If you are the **first user** of the day: |
| - | * Turn on the computer (if needed) | + | * Turn off Components |
| + | {{::zeiss_lsm_980_components.png?100|}} | ||
| + | * Turn off Main Switch | ||
| + | {{::zeiss_lsm_980_main_switch.png?100|}} | ||
| + | * Turn on the computer | ||
| + | * Turn on Main Switch | ||
| + | * Turn on Components | ||
| + | * Log into Windows with your Agendo credentials | ||
| + | * Start **ZEN** | ||
| + | Else: | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| * Start **ZEN** | * Start **ZEN** | ||
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| ===== How to end your session ===== | ===== How to end your session ===== | ||
| - | If there is another user for this microscope in the __next hour__: | + | If there is another user: |
| - | * Exit the ZEN software, leave the lasers on | + | |
| - | * Log off the computer | + | |
| - | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | + | |
| - | Else: | + | |
| - | * Turn off the lasers on **ZEN** | + | |
| * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| * Exit the ZEN software | * Exit the ZEN software | ||
| * Log off the computer | * Log off the computer | ||
| - | + | If you are the **last user** of the day: | |
| - | ===== Microscope Turn On Procedure (after complete shutdown) ===== | + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) |
| - | + | ||
| - | If the system is completely off, please follow this procedure: | + | |
| - | + | ||
| - | * Turn on the ''MAIN SWITCH'' | + | |
| - | {{::zeiss_lsm_980_main_switch.png?100|}} | + | |
| - | * Turn on the ''COMPONENTS'' switch | + | |
| - | {{::zeiss_lsm_980_components.png?100|}} | + | |
| - | * Turn on the Laser key | + | |
| - | * Turn on the computer | + | |
| - | * Log into Windows with your Agendo credentials | + | |
| - | * Start the **ZEN** software | + | |
| - | + | ||
| - | ===== Microscope Turn Off Procedure (for complete shutdown) ===== | + | |
| - | + | ||
| - | Please only shutdown the system if instructed to do so by the Bioimaging Staff. | + | |
| - | + | ||
| - | * Turn off the lasers on **ZEN** | + | |
| * Exit the ZEN software | * Exit the ZEN software | ||
| - | * Shutdown the computer | + | * Turn off the computer |
| - | * Turn off the Laser key | + | |
| - | * Turn off the ''COMPONENTS'' switch | + | |
| - | * Turn off the ''MAIN SWITCH'' | + | |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lsm_980.1753800935.txt.gz · Last modified: 2025/07/29 16:55 by bioimaging
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