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zeiss_axioscan_7 [2025/07/19 22:21] bioimaging |
zeiss_axioscan_7 [2025/12/12 11:47] (current) bioimaging [Objectives] |
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| ==== LEDs ==== | ==== LEDs ==== | ||
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| ^ Line ^ Wavelength ^ | ^ Line ^ Wavelength ^ | ||
| - | | UV | 385 nm | | + | | UV | <fc #be00cc>385 nm</fc> | |
| - | | V | 430 nm | | + | | V | <fc #6a00ff>430 nm</fc> | |
| - | | B | 475 nm | | + | | B | <fc #00c0ff>475 nm</fc> | |
| - | | G | 555 nm | | + | | G | <fc #b3ff00>555 nm</fc> | |
| - | | Y | 590 nm | | + | | Y | <fc #ffdf00>590 nm</fc> | |
| - | | R | 630 nm | | + | | R | <fc #ff4f00>630 nm</fc> | |
| - | | FR | 735 nm | | + | | FR | <fc #be0000>735 nm</fc> | |
| ==== Filtersets ==== | ==== Filtersets ==== | ||
| - | ^ Filterset ^ Reference ^ Dichroic ^ Emission ^ | + | ^ Filterset ^ Dichroic ^ Emission ^ Reference ^ |
| - | | LED SBP | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489112-9010-000|112 SBP]] | 405 nm | 410-440 nm | | + | | LED SBP | 405 nm | <fc #5400ff>410-440 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489112-9010-000|FS112SBP]] | |
| - | | ::: | ::: | 493 nm | 500-530 nm | | + | | ::: | 493 nm | <fc #1fff00>500-530 nm</fc> | ::: | |
| - | | ::: | ::: | 575 nm | 580-605 nm | | + | | ::: | 575 nm | <fc #ffd900>580-605 nm</fc> | ::: | |
| - | | ::: | ::: | 654 nm | 660-705 nm | | + | | ::: | 654 nm | <fc #ff0000>660-705 nm</fc> | ::: | |
| - | | ::: | ::: | 761 nm | 769-807 nm | | + | | ::: | 761 nm | <fc #9b0000>769-807 nm</fc> | ::: | |
| - | + | ||
| - | + | ||
| - | Check here to check your experimental design in [[https://www.fpbase.org/spectra/?s=7754,222,223,204,205,2055,2057,262,263,2059,2068,306,307,$cf0_BP_425_30_90,$cf1_BP_515_30_90,$cf2_BP_593_25_90,$cf3_BP_683_45_90&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength | FPbase.org for a sample stained with DAPI+A488+A568+A647 (change at will!)]] | + | |
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 5x | Fluar | Air | 0.25 | 12.5 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420130-9900-000/Objective-Fluar-5x-0.25-M27|420130-9900-000]] | | + | | <fc #df2b37>5x</fc> | Fluar | Air | 0.25 | 12.5 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420130-9900-000/Objective-Fluar-5x-0.25-M27|420130-9900-000]] | |
| - | | 10x | Plan-Apochromat | Air | 0.45 | 2.1 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | | + | | <fc #fef042>10x</fc> | Plan-Apochromat | Air | 0.45 | 2.1 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | |
| - | | 20x | Plan-Apochromat | Air | 0.8 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | | + | | <fc #0aa05c>20x</fc> | Plan-Apochromat | Air | 0.8 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | |
| - | | 40x | Plan-Apochromat | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | + | | <fc #01b3f5>40x</fc> | Plan-Apochromat | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | |
| ==== Cameras ==== | ==== Cameras ==== | ||
zeiss_axioscan_7.1752956486.txt.gz · Last modified: 2025/07/19 22:21 by bioimaging
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