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zeiss_axioscan_7 [2025/01/05 20:01] bioimaging |
zeiss_axioscan_7 [2026/02/13 21:50] (current) bioimaging [Filtersets] |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axioscan_7_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axioscan_7_wiki_2024.png" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axioscan_7_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axioscan_7_wiki_2024.png" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axioscan_7_map_2024.png">Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axioscan_7_map_2024.png">[Lisbon] Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| - | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/imaging-systems/axioscan-for-biology.htm">Axioscan 7</a><br> | + | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/imaging-systems/axioscan-for-biology.html">Axioscan 7</a><br> |
| <b>Nickname</b>: "Axioscan"<br> | <b>Nickname</b>: "Axioscan"<br> | ||
| - | <b>Software</b>: ZEN 3.9 (ZEN Slidescan)<br> | + | <b>Software</b>: ZEN 3.12 (ZEN Slidescan)<br> |
| <b>Year</b>: 2024 <br> | <b>Year</b>: 2024 <br> | ||
| <b>SN</b>: 4661000477<br> | <b>SN</b>: 4661000477<br> | ||
| Line 33: | Line 33: | ||
| <html> | <html> | ||
| - | <p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/u-tnLKIbxnk" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> | + | <p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/u-tnLKIbxnk?si=Prp-SfxlzOZyTN89" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" referrerpolicy="strict-origin-when-cross-origin" allowfullscreen></iframe></p> |
| </html> | </html> | ||
| - | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| ===== System components ===== | ===== System components ===== | ||
| ==== LEDs ==== | ==== LEDs ==== | ||
| - | ^ Line ^ Wavelength ^ | ||
| - | | UV | 385 nm | | ||
| - | | V | 430 nm | | ||
| - | | B | 475 nm | | ||
| - | | G | 555 nm | | ||
| - | | Y | 590 nm | | ||
| - | | R | 630 nm | | ||
| - | | FR | 735 nm | | ||
| + | ^ Line ^ Wavelength ^ | ||
| + | | UV | <fc #be00cc>385 nm</fc> | | ||
| + | | V | <fc #6a00ff>430 nm</fc> | | ||
| + | | B | <fc #00c0ff>475 nm</fc> | | ||
| + | | G | <fc #b3ff00>555 nm</fc> | | ||
| + | | Y | <fc #ffdf00>590 nm</fc> | | ||
| + | | R | <fc #ff4f00>630 nm</fc> | | ||
| + | | FR | <fc #be0000>735 nm</fc> | | ||
| ==== Filtersets ==== | ==== Filtersets ==== | ||
| - | ^ Filterset ^ Reference ^ Dichroic ^ Emission ^ | + | ^ Filterset ^ Dichroic ^ Emission ^ Reference ^ |
| - | | LED SBP | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489112-9010-000|112 SBP]] | 405 nm | 410-440 nm | | + | | LED SBP | 405 nm | <fc #5400ff>410-440 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489112-9110-400|FS112SBP]] | |
| - | | ::: | ::: | 493 nm | 500-530 nm | | + | | ::: | 493 nm | <fc #1fff00>500-530 nm</fc> | ::: | |
| - | | ::: | ::: | 575 nm | 580-605 nm | | + | | ::: | 575 nm | <fc #ffd900>580-605 nm</fc> | ::: | |
| - | | ::: | ::: | 654 nm | 660-705 nm | | + | | ::: | 654 nm | <fc #ff0000>660-705 nm</fc> | ::: | |
| - | | ::: | ::: | 761 nm | 769-807 nm | | + | | ::: | 761 nm | <fc #9b0000>769-807 nm</fc> | ::: | |
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 5x | Fluar | Air | 0.25 | 12.5 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420130-9900-000/Objective-Fluar-5x-0.25-M27|420130-9900-000]] | | + | | <fc #df2b37>5x</fc> | Fluar | Air | 0.25 | 12.5 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420130-9900-000/Objective-Fluar-5x-0.25-M27|420130-9900-000]] | |
| - | | 10x | Plan-Apochromat | Air | 0.45 | 2.1 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | | + | | <fc #fef042>10x</fc> | Plan-Apochromat | Air | 0.45 | 2.1 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | |
| - | | 20x | Plan-Apochromat | Air | 0.8 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | | + | | <fc #0aa05c>20x</fc> | Plan-Apochromat | Air | 0.8 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | |
| - | | 40x | Plan-Apochromat | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | + | | <fc #01b3f5>40x</fc> | Plan-Apochromat | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | |
| ==== Cameras ==== | ==== Cameras ==== | ||
| Line 74: | Line 74: | ||
| * Turn on the Axioscan 7 | * Turn on the Axioscan 7 | ||
| - | * Turn on the computer | + | * Turn on the computer (if needed) |
| * Login in to Windows with your Agendo credentials | * Login in to Windows with your Agendo credentials | ||
| * Start the **ZEN** software | * Start the **ZEN** software | ||
| Line 81: | Line 81: | ||
| If there is another user for this microscope is the __next hour__: | If there is another user for this microscope is the __next hour__: | ||
| * Exit the ZEN software | * Exit the ZEN software | ||
| - | * Log off Windows | + | * Log off the computer |
| Else | Else | ||
| * Exit the ZEN software | * Exit the ZEN software | ||
zeiss_axioscan_7.1736103700.txt.gz · Last modified: 2025/01/05 20:01 by bioimaging
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