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quality [2022/09/01 15:05] bioimaging |
quality [2024/10/12 21:24] (current) bioimaging |
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- | {{:quality_control_neg.png|}} | + | {{:quality_control_section.png|}} |
{{ ::psfj_fit.png?150|}} | {{ ::psfj_fit.png?150|}} | ||
{{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | {{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | ||
- | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ | + | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[https://biii.eu/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ |
\\ | \\ | ||
**Illumination**\\ | **Illumination**\\ | ||
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=== Point Scanning Confocal Microscopes === | === Point Scanning Confocal Microscopes === | ||
- | [[zeiss_lsm_710_quality|{{::zeiss_lsm_710_with_name.png?160|}}]] [[zeiss_lsm_880_quality|{{::zeiss_lsm_880_with_name.png?160|}}]] [[zeiss_lsm_980_quality|{{::zeiss_lsm_980_with_name.png?160|}}]] | + | [[zeiss_lsm_980_quality|{{::zeiss_lsm_980_with_name.png?160|}}]] [[zeiss_lsm_880_quality|{{::zeiss_lsm_880_with_name.png?160|}}]] |
=== Multi-photon Microscope === | === Multi-photon Microscope === | ||
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=== No longer available... === | === No longer available... === | ||
+ | * [[zeiss_lsm_710_quality|Zeiss LSM 710]] (retired) | ||
* [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired) | * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired) | ||
* [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired) | * [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired) |