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quality [2022/01/18 20:51] bioimaging |
quality [2024/10/12 21:24] (current) bioimaging |
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| - | {{:quality_control_neg.png|}} | + | {{:quality_control_section.png|}} |
| {{ ::psfj_fit.png?150|}} | {{ ::psfj_fit.png?150|}} | ||
| {{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | {{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ | ||
| - | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ | + | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[https://biii.eu/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ |
| \\ | \\ | ||
| **Illumination**\\ | **Illumination**\\ | ||
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| === Point Scanning Confocal Microscopes === | === Point Scanning Confocal Microscopes === | ||
| - | [[zeiss_lsm_710_quality|{{::zeiss_lsm_710_with_name.png?160|}}]] [[zeiss_lsm_880_quality|{{::zeiss_lsm_880_with_name.png?160|}}]] | + | [[zeiss_lsm_980_quality|{{::zeiss_lsm_980_with_name.png?160|}}]] [[zeiss_lsm_880_quality|{{::zeiss_lsm_880_with_name.png?160|}}]] |
| === Multi-photon Microscope === | === Multi-photon Microscope === | ||
| [[leica_sp8_mp_quality|{{::leica_sp8_mp_with_name.png?160|}}]] | [[leica_sp8_mp_quality|{{::leica_sp8_mp_with_name.png?160|}}]] | ||
| - | |||
| - | === Light Sheet Fluorescence Microscope === | ||
| - | |||
| - | [[zeiss_lightsheet_z1_quality|{{::zeiss_lightsheet_z1_with_name.png?160|}}]] | ||
| === Widefield Fluorescence Microscopes === | === Widefield Fluorescence Microscopes === | ||
| - | [[zeiss_cell_observer_quality|{{::zeiss_cell_observer_with_name.png?160|}}]] [[zeiss_axiovert_200m_quality|{{::zeiss_axiovert_200m_with_name.png?160|}}]] [[nikon_eclipse_ti_quality|{{::nikon_eclipse_ti_with_name.png?160|}}]] [[leica_dm5000b_quality|{{::leica_dm5000b_with_name.png?160|}}]] | + | [[zeiss_cell_observer_quality|{{::zeiss_cell_observer_with_name.png?160|}}]] [[zeiss_axiovert200m_quality|{{::zeiss_axiovert_200m_with_name.png?160|}}]] [[nikon_eclipse_ti_quality|{{::nikon_eclipse_ti_with_name.png?160|}}]] [[leica_dm5000b_quality|{{::leica_dm5000b_with_name.png?160|}}]] |
| === Fluorescence Stereo Microscope === | === Fluorescence Stereo Microscope === | ||
| Line 43: | Line 39: | ||
| === No longer available... === | === No longer available... === | ||
| + | * [[zeiss_lsm_710_quality|Zeiss LSM 710]] (retired) | ||
| * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired) | * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired) | ||
| * [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired) | * [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired) | ||