quality
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quality [2020/03/01 23:16] bioimaging |
quality [2024/10/12 21:24] (current) bioimaging |
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| - | ====== Microscope Quality Control ====== | + | {{:quality_control_section.png|}} |
| - | {{::psfj_fit.png?150 |}} **PSFs** \\ | + | {{ ::psfj_fit.png?150|}} |
| - | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[http://www.knoplab.de/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ | + | {{::imm_question.jpg?140 |Image by Maria Henriques, João Ferreira and José Rino}} **PSFs** \\ |
| + | We image sub-resolution fluorescent beads (0.17 μm diameter) and use [[https://biii.eu/psfj|PSFj]] to analyze the acquired Point Spread Functions (PSFs) and determine the resolution in x, y and z for each objective.\\ | ||
| \\ | \\ | ||
| **Illumination**\\ | **Illumination**\\ | ||
| - | We measure the intensity of fluorescent lamps and laser lines in each system. | + | We measure the intensity of laser lines, LEDs and fluorescent lamps for each system. |
| + | \\ | ||
| + | \\ | ||
| \\ | \\ | ||
| - | ==== Check the Systems ==== | ||
| === Spinning Disc Confocal Microscopes === | === Spinning Disc Confocal Microscopes === | ||
| - | * [[3i_Marianas SDC_quality|3i Marianas SDC]] | + | |
| - | * [[zeiss_cell_observer_sd_quality|Zeiss Cell Observer SD]] | + | [[3i_marianas_sdc_quality|{{::3i_marianas_sdc_with_name.png?160|}}]] [[zeiss_cell_observer_sd_quality|{{::zeiss_cell_observer_sd_with_name.png?160|}}]] |
| === Point Scanning Confocal Microscopes === | === Point Scanning Confocal Microscopes === | ||
| - | * [[zeiss_lsm_880_quality|Zeiss LSM 880]] | + | |
| - | * [[zeiss_lsm_710_quality|Zeiss LSM 710]] | + | [[zeiss_lsm_980_quality|{{::zeiss_lsm_980_with_name.png?160|}}]] [[zeiss_lsm_880_quality|{{::zeiss_lsm_880_with_name.png?160|}}]] |
| === Multi-photon Microscope === | === Multi-photon Microscope === | ||
| - | * [[leica_sp8_mp_quality|Leica SP8 MP]] | + | |
| + | [[leica_sp8_mp_quality|{{::leica_sp8_mp_with_name.png?160|}}]] | ||
| === Widefield Fluorescence Microscopes === | === Widefield Fluorescence Microscopes === | ||
| - | * [[zeiss_cell_observer_quality|Zeiss Cell Observer]] | + | |
| - | * [[zeiss_axiovert200m_quality|Zeiss Axiovert 200M]] | + | [[zeiss_cell_observer_quality|{{::zeiss_cell_observer_with_name.png?160|}}]] [[zeiss_axiovert200m_quality|{{::zeiss_axiovert_200m_with_name.png?160|}}]] [[nikon_eclipse_ti_quality|{{::nikon_eclipse_ti_with_name.png?160|}}]] [[leica_dm5000b_quality|{{::leica_dm5000b_with_name.png?160|}}]] |
| + | |||
| + | === Fluorescence Stereo Microscope === | ||
| + | |||
| + | [[zeiss_axiozoom_v16_quality|{{::zeiss_axiozoom_v16_with_name.png?160|}}]] | ||
| + | |||
| + | ==== Other Imaging Systems ==== | ||
| + | |||
| + | [[ivis_lumina_quality|{{::ivis_lumina_with_name.png?160|}}]] | ||
| ---- | ---- | ||
| === No longer available... === | === No longer available... === | ||
| + | * [[zeiss_lsm_710_quality|Zeiss LSM 710]] (retired) | ||
| * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired) | * [[zeiss_lsm_7_live_quality|Zeiss LSM 7 Live]] (retired) | ||
| * [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired) | * [[Zeiss_LSM 510 META_quality|LSM 510 META]] (retired) | ||
| ---- | ---- | ||
| - | [[resources|Back to the Resources page]] | + | [[start|Back to the Bioimaging Wiki]] |
quality.1583100998.txt.gz · Last modified: 2020/03/01 23:16 by bioimaging
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