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prairie [2026/02/17 18:15] bioimaging [Filtersets (Ocular)] |
prairie [2026/02/17 18:18] (current) bioimaging |
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| ==== Objectives ==== | ==== Objectives ==== | ||
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| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD ^ Reference ^ | ||
| | <fc #0aa05c>20x</fc> | Olympus XLUM FL Plan | Water (dipping) | 1.00 | 2.00 mm | [[https://www.thorlabs.com/thorproduct.cfm?partnumber=N20X-PFH|N20X-PFH]] | | | <fc #0aa05c>20x</fc> | Olympus XLUM FL Plan | Water (dipping) | 1.00 | 2.00 mm | [[https://www.thorlabs.com/thorproduct.cfm?partnumber=N20X-PFH|N20X-PFH]] | | ||
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| ==== Emission Filters (multi-photon) ==== | ==== Emission Filters (multi-photon) ==== | ||
| - | {{::xxfiltersetDiagram.png |}} | ||
| - | \\ | ||
| ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ||
| - | | CH1 (PMT) | Red | 640/680 nm | Alexa647, Cy5 | | + | | CH1 (PMT) | Red | <fc #ff0000>640-680 nm</fc> | Alexa647, Cy5 | |
| - | | Ch2 (GaAsP) | Green | 570-620 nm | mCherry, Alexa568, Cy3 | | + | | Ch2 (GaAsP) | Green | <fc #ffcf00>570-620 nm</fc> | mCherry, Alexa568, Cy3 | |
| - | | Ch3 (GaAsP) | Far Red | 500-550 nm | GFP, Alexa488, FITC | | + | | Ch3 (GaAsP) | Far Red | <fc #4aff00>500-550 nm</fc> | GFP, Alexa488, FITC | |
| - | | Ch4 (PMT)* | Blue | 435-485 nm | DAPI*, CFP or SHG | | + | | Ch4 (PMT)* | Blue | <fc #007bff>435-485 nm</fc> | DAPI*, CFP or SHG | |
| *Ch4 can be used either in fluorescence mode, or alternatively as a BF ("DODT") channel or for SHG - ask staff | *Ch4 can be used either in fluorescence mode, or alternatively as a BF ("DODT") channel or for SHG - ask staff | ||
| - | \\ | ||
| - | **Upon request:** | ||
| - | \\ (requires changing filters and the dichroic beamsplitter) | ||
| - | |||
| - | UNDER CONSTRUCTION!!! | ||
| ===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
prairie.1771348544.txt.gz · Last modified: 2026/02/17 18:15 by bioimaging
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