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prairie [2026/02/17 18:13] bioimaging |
prairie [2026/02/17 18:18] (current) bioimaging |
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| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | |
| - | | 20x | Olympus (now Evident!) XLUM FL Plan | Water (dipping) | 1.00 | 2.00 | [[https://www.thorlabs.com/thorproduct.cfm?partnumber=N20X-PFH]] | | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD ^ Reference ^ |
| + | | <fc #0aa05c>20x</fc> | Olympus XLUM FL Plan | Water (dipping) | 1.00 | 2.00 mm | [[https://www.thorlabs.com/thorproduct.cfm?partnumber=N20X-PFH|N20X-PFH]] | | ||
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| ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ Fluorochromes ^ | ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ Fluorochromes ^ | ||
| | 2p (pos1) | - | mirror | - | - | all | | | 2p (pos1) | - | mirror | - | - | all | | ||
| - | | Green (pos3) | - | blue | - | ~525 nm | GFP, FITC, Alexa488 | | + | | Green (pos3) | - | blue | - | ~ <fc #4aff00>525 nm</fc> | GFP, FITC, Alexa488 | |
| - | | Orange (pos4) | - | green | - | ~580 nm | TRITC, Alexa568 | | + | | Orange (pos4) | - | green | - | ~ <fc #ffff00>580 nm</fc> | TRITC, Alexa568 | |
| - | | Blue (pos5) | - | violet | - | ~460 nm | DAPI | | + | | Blue (pos5) | - | violet | - | ~ <fc #007bff>460 nm</fc> | DAPI | |
| ==== Emission Filters (multi-photon) ==== | ==== Emission Filters (multi-photon) ==== | ||
| - | {{::xxfiltersetDiagram.png |}} | ||
| - | \\ | ||
| ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ||
| - | | CH1 (PMT) | Red | 640/680 nm | Alexa647, Cy5 | | + | | CH1 (PMT) | Red | <fc #ff0000>640-680 nm</fc> | Alexa647, Cy5 | |
| - | | Ch2 (GaAsP) | Green | 570-620 nm | mCherry, Alexa568, Cy3 | | + | | Ch2 (GaAsP) | Green | <fc #ffcf00>570-620 nm</fc> | mCherry, Alexa568, Cy3 | |
| - | | Ch3 (GaAsP) | Far Red | 500-550 nm | GFP, Alexa488, FITC | | + | | Ch3 (GaAsP) | Far Red | <fc #4aff00>500-550 nm</fc> | GFP, Alexa488, FITC | |
| - | | Ch4 (PMT)* | Blue | 435-485 nm | DAPI*, CFP or SHG | | + | | Ch4 (PMT)* | Blue | <fc #007bff>435-485 nm</fc> | DAPI*, CFP or SHG | |
| *Ch4 can be used either in fluorescence mode, or alternatively as a BF ("DODT") channel or for SHG - ask staff | *Ch4 can be used either in fluorescence mode, or alternatively as a BF ("DODT") channel or for SHG - ask staff | ||
| - | \\ | ||
| - | **Upon request:** | ||
| - | \\ (requires changing filters and the dichroic beamsplitter) | ||
| - | |||
| - | UNDER CONSTRUCTION!!! | ||
| ===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
prairie.1771348397.txt.gz · Last modified: 2026/02/17 18:13 by bioimaging
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