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prairie [2025/01/11 17:03] bioimaging |
prairie [2026/02/17 18:18] (current) bioimaging |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=prairie.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=prairie.jpg" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=prairie.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=prairie.jpg" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: [Oeiras] 0B06 | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_zeiss_prairie.png">[Oeiras] Room 0B06</a> |
| <br><b>Manufacturer</b>: Prairie (now Bruker!)<br> | <br><b>Manufacturer</b>: Prairie (now Bruker!)<br> | ||
| <b>Model</b>: Prairie Ultima <br> | <b>Model</b>: Prairie Ultima <br> | ||
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|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=leica_sp8_mp_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> XXX Quality Control</a> | + | → <a href="/facility/bioimaging/doku.php?id=leica_sp8_mp_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Prairie Multiphoton Quality Control</a> |
| <br> | <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=leica_sp8_mp_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> XXX Usage Statistics</a> | + | → <a href="/facility/bioimaging/doku.php?id=leica_sp8_mp_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Prairie Multiphoton Usage Statistics</a> |
| </p> | </p> | ||
| </td> | </td> | ||
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| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | |
| - | | 20x | Olympus (now Evident!) XLUM FL Plan | Water (dipping) | 1.00 | 2.00 | [[https://www.thorlabs.com/thorproduct.cfm?partnumber=N20X-PFH]] | | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD ^ Reference ^ |
| + | | <fc #0aa05c>20x</fc> | Olympus XLUM FL Plan | Water (dipping) | 1.00 | 2.00 mm | [[https://www.thorlabs.com/thorproduct.cfm?partnumber=N20X-PFH|N20X-PFH]] | | ||
| Line 52: | Line 53: | ||
| ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ Fluorochromes ^ | ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ Fluorochromes ^ | ||
| | 2p (pos1) | - | mirror | - | - | all | | | 2p (pos1) | - | mirror | - | - | all | | ||
| - | | Green (pos3) | - | blue | - | ~525 nm | GFP, FITC, Alexa488 | | + | | Green (pos3) | - | blue | - | ~ <fc #4aff00>525 nm</fc> | GFP, FITC, Alexa488 | |
| - | | Orange (pos4) | - | green | - | ~580 nm | TRITC, Alexa568 | | + | | Orange (pos4) | - | green | - | ~ <fc #ffff00>580 nm</fc> | TRITC, Alexa568 | |
| - | | Blue (pos5) | - | violet | - | ~460 nm | DAPI | | + | | Blue (pos5) | - | violet | - | ~ <fc #007bff>460 nm</fc> | DAPI | |
| ==== Emission Filters (multi-photon) ==== | ==== Emission Filters (multi-photon) ==== | ||
| - | {{::xxfiltersetDiagram.png |}} | ||
| - | \\ | ||
| ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ^ Detector ^ Filter ^ Transmission ^ Fluorochromes ^ | ||
| - | | CH1 (PMT) | Red | 640/680 nm | Alexa647, Cy5 | | + | | CH1 (PMT) | Red | <fc #ff0000>640-680 nm</fc> | Alexa647, Cy5 | |
| - | | Ch2 (GaAsP) | Green | 570-620 nm | mCherry, Alexa568, Cy3 | | + | | Ch2 (GaAsP) | Green | <fc #ffcf00>570-620 nm</fc> | mCherry, Alexa568, Cy3 | |
| - | | Ch3 (GaAsP) | Far Red | 500-550 nm | GFP, Alexa488, FITC | | + | | Ch3 (GaAsP) | Far Red | <fc #4aff00>500-550 nm</fc> | GFP, Alexa488, FITC | |
| - | | Ch4 (PMT)* | Blue | 435-485 nm | DAPI*, CFP or SHG | | + | | Ch4 (PMT)* | Blue | <fc #007bff>435-485 nm</fc> | DAPI*, CFP or SHG | |
| *Ch4 can be used either in fluorescence mode, or alternatively as a BF ("DODT") channel or for SHG - ask staff | *Ch4 can be used either in fluorescence mode, or alternatively as a BF ("DODT") channel or for SHG - ask staff | ||
| - | \\ | ||
| - | **Upon request:** | ||
| - | \\ (requires changing filters and the dichroic beamsplitter) | ||
| - | |||
| - | UNDER CONSTRUCTION!!! | ||
| ===== System Turn On Procedures ===== | ===== System Turn On Procedures ===== | ||
| Line 85: | Line 79: | ||
| ===== System turn Off procedures ===== | ===== System turn Off procedures ===== | ||
| - | Transfer your data to the institutional server (data is deleted every month) | + | * Transfer your data to the institutional server (data is deleted every 3 months) |
| + | * Clean up immersion objective(s) | ||
| + | * Remove all your samples and accessories and leave the working area ready for the next user | ||
| If there is another user for this microscope in the __next hour__: | If there is another user for this microscope in the __next hour__: | ||
| - | * Leave the fluorescent lamp and lasers on | + | * Leave the fluorescent lamp and LASER "ON" |
| - | * Clean up immersion objective(s) and inform staff you are leaving the system "on" | + | * Inform staff you are leaving the system "ON" |
| + | |||
| Else: | Else: | ||
| - | * Set the laser to ''standby'' | + | * Set the LASER to "Standby" (LASER key to the left) |
| * Turn "OFF" all pre-amps (make sure to protect 1st for Ch1&2! | * Turn "OFF" all pre-amps (make sure to protect 1st for Ch1&2! | ||
| - | * Turn off the fluorescence lamp | + | * Turn "OFF" the fluorescence lamp |
| - | * Turn off monitor, lights, and close the microscope curtains | + | * Turn "OFF" monitor, lights, and close the microscope curtains |
| - | * Make sure you turn off any life support unit used (heating, CO2, O2...) | + | * Make sure you turn "OFF" any life support unit used (heating, CO2, O2...) |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
prairie.1736611388.txt.gz · Last modified: 2025/01/11 17:03 by bioimaging
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