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--- oeiras_3i_marianas_sdc [2026/09/05 17:24]
bioimaging
+++ oeiras_3i_marianas_sdc [2026/09/08 01:17] (current)
bioimaging
@@ Line 28: / Line 28: @@
 ===== Microscope overview =====
-{{  :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a combination of confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and specimen to generate point-like illumination covering the whole area “simultaneously”. Each small aperture serves as a detecting pinhole to remove out-of-focus light.
+{{  :yokogawa_sd.png?350|}} The 3i Marianas SDC super-resolution //spinning disk confocal microscope// is a fast imaging system which provides a combination of confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and specimen to generate point-like illumination covering the whole area “simultaneously”. Each small aperture serves as a detecting pinhole to remove out-of-focus light.
-This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 50um), DIC, a removable heating stage with CO2 delivery, and photomanipulation modules (Phasor and Ablate!). The scanhead allows 1x or 2x magnification (for extra magnification and to allow Nyquist sampling and deconvolution).
+This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 50um), DIC, a removable heating stage with CO2 delivery, and photomanipulation modules (Phasor and Ablate!).
+The Super-resolution by Optical Re-Assignment (SoRa) scanhead allows 1x, 2.8x and 4x magnification (for extra magnification and to allow Nyquist sampling and deconvolution). SoRa images have a 1.4x resolution improvement and deconvolved SoRa images have a 2x resolution improvement compared to standard spinning disk data.
   * **Microscope**: [[https://www.microscope.healthcare.nikon.com/products/inverted-microscopes/eclipse-ti2-series/|Nikon Ti2 Eclipse]]
   * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-and-services/life-science/spinning-disk-confocal/csu-w1-confocal-scanner-unit/|Yokogawa CSU-W1]] + [[https://www.yokogawa.com/solutions/products-and-services/life-science/spinning-disk-confocal/uniformizer/#Overview|Uniformizer]]
   * **Camera**: [[https://www.teledynevisionsolutions.com/en-150/products/prime-95b/?segment=tvs&vertical=tvs%20-%20photometrics|Photometrics Prime 95B]]
+  * **Features**
+    * | Super-Resolution by Optical Re-Assignment (SoRa)
+    * | Phasor Holographic Photomanipulation
+    * | Ablate! Laser Ablation System
+    * | Uniformizer - Illumination Field Uniformity
 {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.

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