oeiras_3i_marianas_sdc
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oeiras_3i_marianas_sdc [2025/01/08 15:17] bioimaging [Microscope Turn Off Procedure] |
oeiras_3i_marianas_sdc [2025/01/08 17:36] (current) bioimaging |
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and specimen to generate point-like illumination covering the whole area “simultaneously”. Each small aperture serves as a detecting pinhole to remove out-of-focus light. | + | {{ :yokogawa_sd.png?350|}} The 3i Marianas SDC //spinning disk confocal microscope// is a fast imaging system which provides a combination of confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and specimen to generate point-like illumination covering the whole area “simultaneously”. Each small aperture serves as a detecting pinhole to remove out-of-focus light. |
| This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 50um), DIC, a removable heating stage with CO2 delivery, and photomanipulation modules (Phasor and Ablate!). The scanhead allows 1x or 2x magnification (for extra magnification and to allow Nyquist sampling and deconvolution). | This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 50um), DIC, a removable heating stage with CO2 delivery, and photomanipulation modules (Phasor and Ablate!). The scanhead allows 1x or 2x magnification (for extra magnification and to allow Nyquist sampling and deconvolution). | ||
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| | 594 | 594 nm | 100 mW | | | 594 | 594 nm | 100 mW | | ||
| | 637 | 637 nm | 140 mW | | | 637 | 637 nm | 140 mW | | ||
| + | | Ablate! | 355 nm | 60 μJ/200 Hz | | ||
oeiras_3i_marianas_sdc.1736345879.txt.gz · Last modified: 2025/01/08 15:17 by bioimaging
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