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nikon_htm-hcs [2026/02/19 18:22] bioimaging [Objectives] |
nikon_htm-hcs [2026/09/03 12:15] (current) bioimaging |
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| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_htm-hcs.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_htm-hcs.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_nikon_htm-hcs.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_nikon_htm-hcs.jpg" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_nikon_htm-hcs.png">[Oeiras] Room 0B05</a><br> | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_nikon_htm-hcs.png">[Oeiras] Room 0B05</a><br> | ||
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| The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols. | The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols. | ||
| - | The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an Andor Zyla 4.2 sCMOS camera with 4.0 MPixels with a large field of view capable of maximum of 100 FPS at full frame (2048x2048). | + | The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an [[https://www.biovis.com/images/cameras/SCAS0122E_C11440-42U30.pdf|ORCA-Flash4.0 LT+ sCMOS camera]] with 4.0 MPixels (2048x2048) with a large field of view, low-light sensitivity, and large dynamic range. |
| The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only). | The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only). | ||
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| ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| - | | [[https://andor.oxinst.cn/assets/uploads/products/andor/documents/Zyla-4.2-Specifications-Life-Science.pdf|Andor Zyla 4.2 PLUS]] | 2048 × 2048 | 6.5 x 6.5 | 82% | | + | | [[https://www.biovis.com/images/cameras/SCAS0122E_C11440-42U30.pdf|ORCA-Flash4.0 LT PLUS]] | 2048 × 2048 | 6.5 x 6.5 | 82% | |
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Turn on the microscope using the **switch** on the **bottom of the microscope's optical table**. | * Turn on the microscope using the **switch** on the **bottom of the microscope's optical table**. | ||
| - | {{nikon-htm_turnon_3.png?200|}} | + | {{nikon-htm_turnon_3.png?150|}} |
| + | * Turn on the computer | ||
| + | * Log In into Windows with your Agendo credentials | ||
| + | * Start the **NIS** software | ||
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| + | If there is another user for this microscope in the next hour: | ||
| + | |||
| + | * Exit the **NIS** software | ||
| + | * Log off the computer | ||
| + | * Clean up immersion objectives | ||
| + | |||
| + | Else: | ||
| + | |||
| + | * Exit the **NIS** software | ||
| + | * Clean up immersion objectives | ||
| + | * Turn off the computer | ||
| * Turn off the microscope using the **switch** on the **bottom of the microscope's optical table**. | * Turn off the microscope using the **switch** on the **bottom of the microscope's optical table**. | ||
| - | {{nikon-htm_turnon_3.png?200|}} | + | {{nikon-htm_turnon_3.png?150|}} |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
nikon_htm-hcs.1771521738.txt.gz · Last modified: 2026/02/19 18:22 by bioimaging
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