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nikon_htm-hcs [2025/12/12 10:14] bioimaging |
nikon_htm-hcs [2026/09/03 12:15] (current) bioimaging |
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| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
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| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_htm-hcs.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_htm-hcs.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_nikon_htm-hcs.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_nikon_htm-hcs.jpg" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: [Oeiras] Room 0B05<br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_nikon_htm-hcs.png">[Oeiras] Room 0B05</a><br> |
| <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon</a> <br> | <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon</a> <br> | ||
| <b>Model</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series" target="_blank">Nikon Eclipse Ti</a> <br> | <b>Model</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series" target="_blank">Nikon Eclipse Ti</a> <br> | ||
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| The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols. | The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols. | ||
| - | The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an Andor Zyla 4.2 sCMOS camera with 4.0 MPixels with a large field of view capable of maximum of 100 FPS at full frame (2048x2048). | + | The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an [[https://www.biovis.com/images/cameras/SCAS0122E_C11440-42U30.pdf|ORCA-Flash4.0 LT+ sCMOS camera]] with 4.0 MPixels (2048x2048) with a large field of view, low-light sensitivity, and large dynamic range. |
| The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only). | The optics available include fluorescence filters and brightfield, including DIC (for several magnifications) and phase-contrast (40x LWD only). | ||
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| | <fc #01b3f5>40x</fc> | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]] | 0.162 | 0.108 | | | <fc #01b3f5>40x</fc> | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]] | 0.162 | 0.108 | | ||
| | <fc #01b3f5>40x</fc> | Plan Apo | Air | 0.95 | 0.17-0.25 | Yes | Yes (N2) | No | MRD00400* | 0.161 | 0.107 | | | <fc #01b3f5>40x</fc> | Plan Apo | Air | 0.95 | 0.17-0.25 | Yes | Yes (N2) | No | MRD00400* | 0.161 | 0.107 | | ||
| - | | <fc #01b3f5>40x</fc> | LWD* * | Air | 0.55 | 2.1 | No | No | Yes | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1683|MRP46402]] | 0.161 | 0.108 | | + | | <fc #01b3f5>40x</fc> | ELWD* * | Air | 0.60 | 2.80-3.60 | Yes | No | Yes | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1814|MRH48430]] | 0.161 | 0.108 | |
| | 100x | Plan Apo Lambda | Oil | 1.45 | 0.13 | Yes | Yes (N2) | No | MRD01905* | 0.065 | 0.043 | | | 100x | Plan Apo Lambda | Oil | 1.45 | 0.13 | Yes | Yes (N2) | No | MRD01905* | 0.065 | 0.043 | | ||
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| ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| - | | [[https://andor.oxinst.cn/assets/uploads/products/andor/documents/Zyla-4.2-Specifications-Life-Science.pdf|Andor Zyla 4.2 PLUS]] | 2048 × 2048 | 6.5 x 6.5 | 82% | | + | | [[https://www.biovis.com/images/cameras/SCAS0122E_C11440-42U30.pdf|ORCA-Flash4.0 LT PLUS]] | 2048 × 2048 | 6.5 x 6.5 | 82% | |
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Turn on the microscope using the **switch** on the **bottom of the microscope's optical table**. | * Turn on the microscope using the **switch** on the **bottom of the microscope's optical table**. | ||
| - | {{nikon-htm_turnon_3.png?200|}} | + | {{nikon-htm_turnon_3.png?150|}} |
| + | * Turn on the computer | ||
| + | * Log In into Windows with your Agendo credentials | ||
| + | * Start the **NIS** software | ||
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| + | If there is another user for this microscope in the next hour: | ||
| + | |||
| + | * Exit the **NIS** software | ||
| + | * Log off the computer | ||
| + | * Clean up immersion objectives | ||
| + | |||
| + | Else: | ||
| + | |||
| + | * Exit the **NIS** software | ||
| + | * Clean up immersion objectives | ||
| + | * Turn off the computer | ||
| * Turn off the microscope using the **switch** on the **bottom of the microscope's optical table**. | * Turn off the microscope using the **switch** on the **bottom of the microscope's optical table**. | ||
| - | {{nikon-htm_turnon_3.png?200|}} | + | {{nikon-htm_turnon_3.png?150|}} |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
nikon_htm-hcs.1765530845.txt.gz · Last modified: 2025/12/12 10:14 by bioimaging
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