nikon_htm-hcs
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nikon_htm-hcs [2025/12/12 01:29] bioimaging |
nikon_htm-hcs [2025/12/12 11:29] (current) bioimaging |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_htm-hcs.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_htm-hcs.jpg" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_htm-hcs.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_htm-hcs.jpg" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: [Oeiras] Room 0B05<br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_nikon_htm-hcs.png">[Oeiras] Room 0B05</a><br> |
| <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon</a> <br> | <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon</a> <br> | ||
| <b>Model</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series" target="_blank">Nikon Eclipse Ti</a> <br> | <b>Model</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series" target="_blank">Nikon Eclipse Ti</a> <br> | ||
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| This system is equipped with a CoolLED fluorescence illuminator with 4 excitation lines: | This system is equipped with a CoolLED fluorescence illuminator with 4 excitation lines: | ||
| - | * 380 nm - E.g. DAPI | + | |
| - | * 470 nm - E.g. FITC | + | ^ Line ^ Wavelength ^ |
| - | * 550 nm - E.g. TRITC | + | | UV | <fc #be00cc>380 nm</fc> | |
| - | * 635 nm - E.g. Cy5 | + | | B | <fc #00a9ff>470 nm</fc> | |
| + | | G | <fc #a3ff00>550 nm</fc> | | ||
| + | | R | <fc #ff3900>635 nm</fc> | | ||
| ==== Filtersets Turret 1 (top) ==== | ==== Filtersets Turret 1 (top) ==== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ |
| - | | 1 | Quad-DAPI | | 378/58 | [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|409]] | 432/40 | | + | | 1 | QUAD | 349-407 nm | 409 nm | <fc #3300ff>412-452 nm</fc> | [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|FL-007110]] | |
| - | | 1 | Quad-FITC | | 474/30 | [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|493]] | 515/36 | | + | | ::: | ::: | 459-489 nm | 493 nm | <fc #1fff00>497-533 nm</fc> | ::: | |
| - | | 1 | Quad-TRITC | | 554/26 | [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|573]] | 595/36 | | + | | ::: | ::: | 541-567 nm | 573 nm | <fc #ffcf00>577-613 nm</fc> | ::: | |
| - | | 1 | Quad-Cy5 | | 635/22 | [[https://www.idex-hs.com/store/product-detail/ff409_493_573_652_di02_25x36/fl-007110?search=true|652]] | LP 658 | | + | | ::: | ::: | 624-646 nm | 652 nm | <fc #ff0000>> 658 nm</fc> | ::: | |
| - | | 2 | FITC | | 474/27 | 495 | 525/45 | | + | | 2 | Green | 460-488 nm | 495 nm | <fc #4aff00>503-548 nm</fc> | | |
| - | | 3 | TRITC | | 543/22 | 562 | 593/40 | | + | | 3 | Orange | 532-554 nm | 562 nm | <fc #ffd500>573-613 nm</fc> | | |
| - | | 4 | Cy5 | | 635/18 | 652 | 680/42 | | + | | 4 | Red | 624-642 nm | 652 nm | <fc #ff0000>659-701 nm</fc> | | |
| ==== Filtersets Turret 2 (bottom) ==== | ==== Filtersets Turret 2 (bottom) ==== | ||
| - | ^ Position ^ Filterset ^ | + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ |
| - | | 1 | Empty | | + | | 6 | Fura-2 | 334-346 nm | 400 nm | <fc #00ff28>498-518 nm</fc> | |
| - | | 2 | Empty | | + | | ::: | ::: | 370-390 nm | ::: | ::: | |
| - | | 3 | Empty | | + | |
| - | | 4 | Empty | | + | |
| - | | 5 | Empty | | + | |
| - | | 6 | Fura-2 | | + | |
| - | ==== Fast Filter Wheel Emission filters ==== | + | ==== Emission Filters Fast Filter Wheel ==== |
| - | ^ Filterset ^ Reference ^ Transmission ^ | + | ^ Filterset ^ Emission ^ Reference ^ |
| - | | DAPI-FW | [[https://www.chroma.com/products/parts/at460-50m|at460-50m]] | 460/50 | | + | | DAPI-FW | <fc #007bff>435-485 nm</fc> | [[https://www.chroma.com/products/parts/at460-50m|AT460/50m]] | |
| - | | FITC-FW | [[https://www.chroma.com/products/parts/et525-50m|et525-50m]] | 525/50 | | + | | FITC-FW | <fc #4aff00>500-550 nm</fc> | [[https://www.chroma.com/products/parts/et525-50m|ET525/50m]] | |
| - | | TRITC-FW | [[https://www.chroma.com/products/parts/et595-50m|et595-50m]] | 595/50 | | + | | TRITC-FW | <fc #ffcf00>570-620 nm</fc> | [[https://www.chroma.com/products/parts/et595-50m|ET595/50m]] | |
| - | | Cy5-FW | [[https://www.chroma.com/products/parts/s685-40m|s685-40m]] | 685/40 | | + | | Cy5-FW | <fc #ff0000>665-705 nm</fc> | [[https://www.chroma.com/products/parts/s685-40m|S685/40m]] | |
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ PFS ^ DIC ^ PhC ^ Reference ^ Pixel size (um) (1x zoom) ^ Pixel size (um) (1.5x zoom) ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ PFS ^ DIC ^ PhC ^ Reference ^ Pixel size (um) (1x zoom) ^ Pixel size (um) (1.5x zoom) ^ | ||
| - | | 10x | Plan Apo | Air | 0.45 | 4 | No | Yes (N1) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-179798|MRD70170]] | 0.643 | 0.429 | | + | | <fc #fef042>10x</fc> | Plan Apo | Air | 0.45 | 4 | No | Yes (N1) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-179798|MRD70170]] | 0.643 | 0.429 | |
| - | | 20x | Plan Apo Lambda | Air | 0.75 | 1 | Yes | Yes (N1) | No | MRD00205* | 0.323 | 0.216 | | + | | <fc #0aa05c>20x</fc> | Plan Apo Lambda | Air | 0.75 | 1 | Yes | Yes (N1) | No | MRD00205* | 0.323 | 0.216 | |
| + | | <fc #01b3f5>40x</fc> | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]] | 0.162 | 0.108 | | ||
| + | | <fc #01b3f5>40x</fc> | Plan Apo | Air | 0.95 | 0.17-0.25 | Yes | Yes (N2) | No | MRD00400* | 0.161 | 0.107 | | ||
| + | | <fc #01b3f5>40x</fc> | LWD* * | Air | 0.55 | 2.1 | No | No | Yes | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1683|MRP46402]] | 0.161 | 0.108 | | ||
| | 100x | Plan Apo Lambda | Oil | 1.45 | 0.13 | Yes | Yes (N2) | No | MRD01905* | 0.065 | 0.043 | | | 100x | Plan Apo Lambda | Oil | 1.45 | 0.13 | Yes | Yes (N2) | No | MRD01905* | 0.065 | 0.043 | | ||
| - | | 40x | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]] | 0.162 | 0.108 | | ||
| - | | 40x | Plan Apo | Air | 0.95 | 0.17-0.25 | Yes | Yes (N2) | No | MRD00400* | 0.161 | 0.107 | | ||
| - | | 40x | LWD* * | Air | 0.55 | 2.1 | No | No | Yes | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1683|MRP46402]] | 0.161 | 0.108 | | ||
| * Not fabricated anymore ; * * LWD - Long Working Distance | * Not fabricated anymore ; * * LWD - Long Working Distance | ||
nikon_htm-hcs.1765499383.txt.gz · Last modified: 2025/12/12 01:29 by bioimaging
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