nikon_htm-hcs
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nikon_htm-hcs [2025/02/04 15:22] bioimaging [Objectives] |
nikon_htm-hcs [2025/03/28 17:07] (current) bioimaging [Microscope overview] |
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | The Nikon-HTM/HCS microscope is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols. | + | The Nikon HTM-HCS is a fully automated microscope capable of high-throughput imaging and screening of samples (eg, Ibidi chamber slides or well-plates); with the help of fully programmable and customizable acquisition protocols. |
| The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an Andor Zyla 4.2 sCMOS camera with 4.0 MPixels with a large field of view capable of maximum of 100 FPS at full frame (2048x2048). | The system is equipped with a CoolLED fluorescence illuminator with 4 excitation bands, and an Andor Zyla 4.2 sCMOS camera with 4.0 MPixels with a large field of view capable of maximum of 100 FPS at full frame (2048x2048). | ||
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| ===== Objectives ===== | ===== Objectives ===== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ PFS ^ DIC ^ PhC ^ Reference ^ Pixel size (um) (1x zoom) ^ Pixel size (um) (1.5x zoom) ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ PFS ^ DIC ^ PhC ^ Reference ^ Pixel size (um) (1x zoom) ^ Pixel size (um) (1.5x zoom) ^ | ||
| - | | 10x | Plan Apo | Air | 0.45 | 4 | No | Yes (N1) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-179798|MRD70170]] | 0.643 | | | + | | 10x | Plan Apo | Air | 0.45 | 4 | No | Yes (N1) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-179798|MRD70170]] | 0.643 | 0.429 | |
| - | | 20x | Plan Apo Lambda | Air | 0.75 | 1 | Yes | Yes (N1) | No | MRD00205* | 0.323 | | | + | | 20x | Plan Apo Lambda | Air | 0.75 | 1 | Yes | Yes (N1) | No | MRD00205* | 0.323 | 0.216 | |
| | 100x | Plan Apo Lambda | Oil | 1.45 | 0.13 | Yes | Yes (N2) | No | MRD01905* | 0.065 | 0.043 | | | 100x | Plan Apo Lambda | Oil | 1.45 | 0.13 | Yes | Yes (N2) | No | MRD01905* | 0.065 | 0.043 | | ||
| | 40x | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]] | 0.162 | 0.108 | | | 40x | Plan Fluor | Oil | 1.3 | 0.24 | Yes | Yes (N2) | No | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1829|MRH01401]] | 0.162 | 0.108 | | ||
| - | | 40x | Plan Apo | Air | 0.95 | 0.17-0.25 | Yes | Yes (N2) | No | MRD00400* | 0.161 | | | + | | 40x | Plan Apo | Air | 0.95 | 0.17-0.25 | Yes | Yes (N2) | No | MRD00400* | 0.161 | 0.107 | |
| - | | 40x | LWD* * | Air | 0.55 | 2.1 | No | No | Yes | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1683|MRP46402]] | 0.161 | | | + | | 40x | LWD* * | Air | 0.55 | 2.1 | No | No | Yes | [[https://www.microscope.healthcare.nikon.com/en_EU/products/optics/selector/comparison/-1683|MRP46402]] | 0.161 | 0.108 | |
| * Not fabricated anymore ; * * LWD - Long Working Distance | * Not fabricated anymore ; * * LWD - Long Working Distance | ||
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| + | The Bioimaging Platform **highly advises against using the 40x LWD objective with 1.5x zoom** | ||
| ===== Camera ===== | ===== Camera ===== | ||
nikon_htm-hcs.1738678948.txt.gz · Last modified: 2025/02/04 15:22 by bioimaging
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